| Project/Area Number |
10044249
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
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Principal Investigator |
TANAKA Akira TOKYOMEDICALAND DENTALUNIVERSITY SCHOOLOFMEDICINE LECTURER, 医学部・附属病院, 講師 (70171733)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Michiro TOKYOMEDICALAND DENTALUNIVERSITY SCHOOLOFMEDICINE ASSISTANTPROF., 医学部・附属病院, 助手 (10301152)
KOBAYASHI Yasushi TOKYOMEDICALAND DENTALUNIVERSITY SCHOOLOFMEDICINE ASSISTANTPROF., 医学部・附属病院, 助手 (70225548)
ジアンターコ サンドラ・ アラバマ大学, 医学部, 教授
ブラドレイ ウイリアム・ アラバマ大学, 医学部, 教授
ハーベル リチャード・ジ カルフォルニア大学, 心臓血管研究所, 教授
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥20,700,000 (Direct Cost: ¥20,700,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1999: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | ApoB48 receptor / Postprandialhyperlipidemia / Atherosclerosis / TG rich lipoprotein / Immunohistochemical stain / RLP cholesterol / Remnant / Insulin resistance / coronary artery disease / 脂肪負荷試験 / アポB48レセプター / RLP |
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| Research Abstract |
Unique receptor, apo B48 receptor, was identified in human monocyte-macrophages. The receptor binds and internizes triglyceride(TG)rich Lipoproteins via a domain in apo B48. The apo B48 receptor cDNA sequences were elucidated. Immunohistochemical studies indicate colocalization of anti-apo B48 receptor Ab and monocyte-macrophage specific Ab in human atherosclerotic lesion foam cells. This suggests that apo B48 receptor may contribute to foam cell formation and atherosclerosis in abnormal state with elevated TG-rich lipoproteins.
Apo B100 expression in the epithelial cells of small intestine were identified by immunoblotting and dot-blotting of PCR-amplified cDNA.It suggests that dietary VLDL may be synthesized in human small intestine epithelium and converted into LDL, atherogenic lipoprotein.
Some cinical studies showed that increase of remnants(RLP cholesterol), especially in postprandial state, has an important role in developimg atherosclerosis.
Remnants(RLP cholesterol)were indicated to increases in the state of insulin resistance.
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