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Joint Research on Acidic Glycoconjugates on Cell Surfaces

Research Project

Project/Area Number 10044265
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

KITAJIMA Kenji  Nagoya Univ., Graduate School of Bioagri. Sci., Associate Professor, 大学院・生命農学研究科, 助教授 (80192558)

Co-Investigator(Kenkyū-buntansha) SATO Chihiro  Japan Society for Promotion of Science, PD Fellow, 日本学術振興会, 特別研究員PD
MATSUDA Tsukasa  Nagoya Univ., Graduate School of Bioagri. Sci., Professor, 大学院・生命農学研究科, 教授 (20144131)
COLLEY Karen  イリノイ大学, 医学部, 助教授
TROY Frederi  カリフォルニア大学, 医学部, 教授
LENNARZ Will  ニューヨーク州立大学, 生化学細胞生物学部門, 教授
井上 康男  台湾中央研究院, 生物化学研究所, 教授
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsPOLYSIALIC ACID / OLIGOSIALIC ACID / KDN / SULFATED GLYCAN / FERTILIZATION / SURFACE GLYCANS TIMOR CELLS / METABOLISM OF SIALIC ACID / GLYCOLIPID-ENRICHED MICRODOMAIN / 神経細胞接着分子
Research Abstract

The objective of this research project was to elucidation of regulatory mechanism for cell recognition and adhesion by acidic glycan chains of cell surface glycoconjugates. The results follow :
1. Clarification of biosynthetic mechanism of oligo/polysialic acid units in brain and nerve system : (1) Several glycoproteins were shown to contain oligo/polysialic acid structures in fetal and adult mammalian brains. Studies on role of this epitope in biological activities in nerve cell system are now underway ; (2) More than 10 new monoclonal antibodies that differentially recognized various di/oligosialic acid with different chain lengths were prepared as probes for function/structure studies. Two highly sensitive chemical methods to detect di/oligosialic acid using fluorescent dye were developed.
2. Role of KDN-glycans or sulfated sialoglycans in gamete interactions at fertilization : (1) The low density detergent-insoluble membrane fractions which were rich in glycolipids were prepared and characterized for unique features of lipid and protein compositions. This membrane fractions are suggested to mediate sperm-egg interaction as adhesion units on the cell surface ; (2) Three distinct cDNAs for sialyltransferases that are involved in synthesis of polysialic and in fish oocyte were cloned ; (3) An avian egg envelope acidic glycoprotein (ZPC) was shown to be biosynthesized in granulosa cells of follicles.
3. Expression and roles of polySia- or KDN-containing glycan chains in tumor cells : (1) KDN-glycan chains were shown to be a oncodevelopmental antigen ; (2) Elevated expression of intracellular KDN in mammalian cells cultured in mannose-rich medium was observed. Some cancer cells whose KDN level is high may have alternations of mannose metabolism ; (3) A cDNA for CMP-KDN synthetase from trout testis was successfully cloned. Substrate specificity with KDN of this type of enzyme is suggested to determine the level of KDN in mammalian normal and malignant cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sato, Chihiro: "Fluorescent-assisted detection of oligosialyl units in glycoconjugates"Analytical Biochemistry. 266. 102-109 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohta, Kaoru: "Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm"Biochem. Biophys. Res. Commun.. 258. 616-623 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Angata, Takashi: "Elevated expression of free deaminoneuraminic acid in mammalian cells cultured in mannose-rich media"Biochem. Biophys. Res. Commun.. 261. 326-331 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Angata, Takashi: "Biosynthesis of KDN. Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis"J. Biol. Chem.. 274. 22949-22956 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitajima, Ken: "Occurrence of α2→8-linked oligosialic acid residues in mammalian glycoproteins"Sialobiology and Other novel Form of Glycosylation. 69-76 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato, Chihiro: "Frequent occurrence of pre-existingα2→8-linked disialic and oligosialic acid with chain lengths up to 7 Sia residues in mammalian brain glydoproteins"J. Biol. Chem.. 275(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chihiro Sato: "Fluorescent-assisted detection of oligosialyl units in glycoconjugates"Analytical Biochemistry. 266. 102-109 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaoru Ohta: "Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm"Biochem. Biochem. Res. Commun.. 258. 616-623 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashi Angata: "Elevated expression of free deaminoneuraminic acid in mammalian cells cultured in mannose-rich media"Biochem. Biochem. Res. Commun.. 261. 326-331 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashi Angata: "Biosynthesis of KDN. Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis"J. Biol. Chem.. 274. 22949-22956 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ken Kitajima: "Occurrence of α2→8-linked oligosialic acid residues in mammalian glycoproteins"Sialobiology and Other novel Form of Glycosylation. 69-76 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chihiro Sato: "Frequent occurrence of pre-existing α2→8-linked disialic and oligosialic acid with chain length up to 7 Sia residues in mammalian brain glycoproteins"J. Biol. Chem. 275(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato,Chihiro: "Fluorescent-assisted detection of oligosialyl units in glycoconjugates."Analytical Biochemistry. 266. 102-109 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohta,Kaoru: "Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm."Biochem.Biophys.Res.Commun.. 258. 616-623 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Angata,Takashi: "Elevated expression of free deaminoneuraminic acid in mammalian cells cultured in mannose-rich media"Biochem.Biophys.Res.Commun.. 261. 326-331 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Angata,Takashi: "Biosynthesis of KDN.Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis."J.Biol.Chem.. 274. 22949-22956 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitajima,Ken: "Occurrence of α2→8-linked oligosialic acid residues in mammalian glycoproteins."Sialobiology and Other novel Form of Glycosylation. 69-76 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato,Chihiro: "Frequent occurrence of pre-existing α2→8-linked disialic and oligosialic acid with chain lengths up to 7 Sia residues in mammalian brain glycoproteins."J.Biol.Chem.. 275(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 北島 健: "新しいシアル酸KDNの糖鎖科学の現状と展望" バイオサイエンスとインダストリー. 56・10. 659-664 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitajima,Ken: "Structure and physiological activities of hyosophorin,a major cortical alveolus glycoprotein of fish eggs." Zygote. 6. S6-S7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fuchizawa,Satoko: "Induction of KDNase Sm,a deaminoneuraminic acid(KDN)residue-specific sialidase from Sphingobacterium multivorum,using synthetic KDN-glycosides." Biochem.Biochem.Res.Commun.248. 505-510 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato,Chihiro: "Development of a highly sensitive chemical method to detect α2→8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane." Analytical Biochemistry. 261. 191-197 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kudo,Mariko: "Cloning and expression of an α2,8-polysialyltransferase(STX)from Xenopus laevis." Glycobiology. 8・8. 771-777 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki,Tadashi: "Peptides glycosylated in the endoplasmic reticulum of yeast are subsequently deglycosylated by a soluble peptide:N-glycanase activity." J.Biol.Chem.273・34. 21526-21530 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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