| Project/Area Number |
10044280
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
OKAMOTO Mitsuhiro Osaka University Medical School, Professor, 医学系研究科, 教授 (90028613)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Osamu Nara Medical University, 講師 (40164850)
TAKEMORI Hiroshi Osaka University Medical School, 医学系研究科, 助手 (90273672)
ESTABROOK Ro テキサス大学, 生化学部, 教授
KAGAWA Norio ヴァンダービルト大学, 生化学部, 助教授
WATERMAN Mic ヴァンダービルト大学, 生化学部, 教授
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Adrenal cortex / Gonads / Steroid hormones / ZOG protein / Cytochrome P450 / 副腎皮質生殖腺 |
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| Research Abstract |
(1) The expression of ZOG/Pref-1 was immunohistochemically studied on the rat embryos. It was expressed in the adreno-gonadal primordium on the embryonic day 12, and after the day 14 its expression was restricted only in the adrenocortical primordiumm. In the adult cortex its expression was localized in the zona glomerulosa.
(2) The cell-type-specific expression of ZOG/Pref-1 gene was studied. The results suggested that nuclear factors other than Sp 1/Egr(s) played important roles for the expression.
(3) CYP11B1 was expressed in Escherichia coli and purified. Its structure/function relationship was studied. Two amino acid residues in its active site, V381 and I384, were found to be important to support its versatile catalytic activity.
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