| Project/Area Number |
10044293
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KATO Yukio Hiroshima University Faculty of Dentistry, Professor, 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
SHEN Ming Hiroshima University Faculty of Dentistry, Assistant, 歯学部, 教務員 (20294572)
NOSIRO Mitsuhide Hiroshima University Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (00144858)
中舛 和子 広島大学, 歯学部, 助手 (00311811)
LEE Kaechoon ハーバード大学, マサチューセッツ総合病院, 講師
V.SEGRE Gino ハーバード大学, マサチューセッツ総合病院, 準教授
来田 吉弘 広島大学, 歯学部, 助手 (10304429)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | chondrocyte / PTHrp / retinoic adic / Indian hedgehog / DEC1 |
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| Research Abstract |
We published a review articule about chondrocyte differentiation using data obtained in this study(Y.Kato et al., in Tissue Enginearing for Therapeutic Use, Oshima and Ikari eds.in press). Our findings indicate that retinol-binding protein is synthesized by chondrocytes in response to PTH, PTHrp or retinoic acid, and that retinol-binding protein is involved partily in mediating the action of PTH and PTHrp on chondrocytes.
We also found that PTHrp directly suppresses Indian hedgehog(Ihh)expression in chondrocytes. This action is a negative feed back mechanism to inhibit excess accumulation of PTHrP and Ihh. We also showed that PTHrp induces CEDP, a novel Rho GEF, and DEC1, a novel transcriptional factor in growth plate chondrocytes. These findings suggest that PTH and PTHrp modulate chondrocyte differentiation and bone formation via changes in retinol-binding protein, CDEP, DEC1 and Ihh.
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