| Project/Area Number |
10044301
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
AKAIKE Norio KYUSHU UNIVERSITY, Grad.Sch.of Med.Sci.Prof., 大学院・医学研究院, 教授 (30040182)
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| Co-Investigator(Kenkyū-buntansha) |
KAKAZU Yasuhiro KYUSHU UNIVERSITY, Grad.Sch.of Med.Sci.Assistant., 大学院・医学研究院, 助手 (70325520)
NABEKURA Junichi KYUSHU UNIVERSITY, Grad.Sch.of Med.Sci.Asso.Prof., 大学院・医学研究院, 助教授 (50237583)
ANDRESEN Michaelc Oregon Health Sciense Univ.Asso.Prof.
李 禎そぶ (李 禎燮) 九州大学, 大学院・医学系研究科, 助手 (50294913)
ANDRESSE M.C Oregon Health Science University, 助教授
野田 百美 九州大学, 医学部, 助手 (80127985)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | synapse / presynaptic nerve ending / synapticbouton / NTS neuron / glutamate / non-NMDAreceptor / ATP receptor / interaction / シナプス・プートン / 循環 / 孤束核 / 神経終末 / パッチクランプ / シナプスボタン標本 / トレーサー / 循環調節 / 中枢性 / 血圧 / 延髄 / 受容体 |
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| Research Abstract |
The carotid sinus reflex of the blood pressure is transmitted to the nucleus of the solitary tract (NTS) via baroreceptor nerve terminals in the aortic depressor nerve (ADN) which is located in the aortic arch. However, not only the detailed mechanisms on synapse transmission in the NTS neurons projected from ADN but also modulations of vasomotor reflex have not been clarified.
In the present studies, the NTS neurons projected from ADN were identified 3-5 days after injection of Di-1 to the separated ADN.This ADN axon from nodose ganglion entered the brainstem to form the solitary tract (ST) and terminated the soma and dendrites of medial NTS neurons, which released glutamate. The identified medial NTS neurons were sensitive to kainate application by 2-fold relative to the non-projected neurons from ADN.ST evoked mono-synaptic glutamatergic responses were mediated by non-NMDA glutamate receptors in the medial NTS neurons. Furthermore, both capsaicin, a VR-1 (vanilloid) receptors agonist and a, b met ATP, a purinergic P2X3 receptors agonist, increased glutamate release 30-fold relative to the control value in dissociated medial NTS neurons, Which is suggested that vasodilation is manipulated capsaicin and ATP at brainstem level.
This functional VR-1 and purinergic regulation might play an important role for any systemic therapies since NTS is exposed to an open blood brain barrier.
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