• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of guanylin family system using knock-out mouse

Research Project

Project/Area Number 10044306
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 内分泌・代謝学
Research InstitutionMiyazaki Medical College

Principal Investigator

NAKAZATO Masamitsu  Miyazaki Medical College, Third Department of Internal Medicine, Full time Lecturer, 医学部 (10180267)

Co-Investigator(Kenkyū-buntansha) 柳沢 正史  テキサス大学, 薬理学, 教授
山口 秀樹  宮崎医科大学, 医学部, 助手 (10305097)
MIYAZATO Mikiya  National cardiovascular Center Research Institute, Department Biochemistry, Inve, 生化学部, 室員 (50291183)
KANGAWA Kenji  National cardiovascular Center Research Institute, Department Biochemistry, Dire, 生化学部, 部長 (00112417)
MATSUKURA Shigeru  Miyazaki Medical College, Third Department of Internal Medicine, Professor, 医学部, 講師 (70030939)
GARBERS David L.  University of Texas Southwestern Medical Center, Department of Pharmacology, Pro
Project Period (FY) 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1998: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsuroguanylin / guanylin / cycle GMP / guanylate cyclase / salt and water metabolism
Research Abstract

Uroguanylin, a member of the guanylin peptide family, acts on guanylyl cyclase C (GC-C) to regulate intestinal and renal fluid and electrolyte transportthrough the second messenger, cyclic GMP.Using an antiserum raised against synthetic rat uroguanylin, we established a radioimmunoassay and identifiedthree endogenous molecular forms : a 15-amino acid uroguanylin, an 18-amino acid uroguanylin that is a monobasic processing product, and a 9.4-kDa prouroguanylin. Prouroguanylin is the major molecular form in these two tissues, whereas only 15-amino-acid uroguanylin is present in the urine.
Uroguanylin is present immunohistochemically in the enterochromaffin cells in the intestine, enterochromaffin-like cells in the stomach, B cells in thepancreatic islets, and tubular epithelial cells in the kidney by immunocytochemical methods using antisera for uroguanylin and prouroguanylin, as well as by hybridization cytochemistry. Uroguanylin has a widespread tissue distribution and is located in cel … More ls which function in an endocrine, paracrine and/or luminocrine (luminal secretion) fashion. The uroguanylin and guanylin genes are arranged in a tail-to-tail array in both species. The respective intergenic distances between the two genes in the human and mouse genomes are approximately 6.5 and 8 kb. The intergenic region between the human uroguanylin and guanylin genes has an Alu sequence and a (CA/FG)19 microsatellite sequence. These findings should help provide a better understandingof the regulatory mechanism of gene expression of the guanylin family peptides, as well as of the molecular mechanism of the evolution of this family.
Plasma uroguanylin levels were significantly higher in patients with heart failure than those in controls (6.7 fmol/ml vs 4.8 fmol/ml). Plasma uroguanylinconcentrations in the coronary sinus and anterior interventricular vein are higher than that in the aorta, suggesting cardiac secretion of uroguanylin into thecirculation. Uroguanylin may function in the regulation of fluid and electrolyte balance in cardiac failure. Less

Report

(2 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Nakazato M, Yamaguchi H, Date Y, Kangawa K, Goy M, Chino N, Matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin." Endocrinology. 139. 5247-5254 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Date Y, Nakazato M, Yamaguchi H, Kangawa K, Kinoshita Y, Chiba T, Ueta Y, Yamashita H, Matsukura S: "Enterochromaffin-like cell, a cellular source of uroguanylin in rat stomach." Endocrinology, in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chino N, Kubo S, Kitani T, Yoshida T, Tanabe R, Kobayashi Y, Nakazato M, Kangawa K, Sakakibara S: "Topological isomers of human uroguanylin : Interconversion between biologically active and inactive isomers." FEBS Lett. 421. 27-31 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita H, Nakazato M, Yamaguchi H, Matsukura S, Fujimoto S, Eto T: "Increased plasma guanylin levels in patients with impaired renal function." Clin Nephrol. 47. 28-32 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyazato M, Nakazato M, Matsukura S, Kangawa K, Matsuo H: "Genomic structure and chromosomal localization of human uroguanylin gene." Genomics. 43. 359-365 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita H, Fujimoto S, Nakazato M, Yokota N, Date Y, Yamaguchi H, Hisanaga S, Eto T: "Plasma and urine levels of uroguanylin and its molecular forms in renal diseases." Kidney Int. 52. 1028-1034 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 中里雅光: "最新内科学大系プログレス2、内分泌・代謝疾患" グアニリンファミリーペプチド., 42-45 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 中里雅光: "消化管ホルモン" 水・NaCl代謝調節に作用する新しい消化管ペプチドグアニリンファミリー,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakazato M,Yamaguchi H,Date Y,Kangawa K,Goy M,Chino N,Matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin" Endocrinology. 139. 5247-5254 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chino N,Kubo S,Kitani T,Yoshida T,Tanabe R,Kobayashi Y,Nakazato M,Kangawa K,Sakakibara S: "Topological isomers of human uroguanylin : Interconversion betwee biologically active and inactive isomers." FEBS Lett. 421. 27-31 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita H,Fujimoto S,Fukae H,Yokota N,Hisanaga S,Nakazato M,Eto T.: "Plasama and urine levels of uroguanylin, a new natriuretic peptide, in nephrotic syndrome." Nephron. 81. 160-164 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Date Y,Nakazato M,Yamaguchi H,Kangawa K,Kinoshita Y,Chiba T,Ueta Y,Yamashita H,Matsukura S: "Enteroc hromaffin-like cell a cellular source of uroguanylin in rat stomach." Endocrinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakazato M. Yamaguchi H.Date Y.Kangawa K. Goy M.Chino N.Matsukura S: "Tissue distribution, cellular source and structural analysis of rat immunoreactive uroguanylin." Endocrinology. 139. 5247-5254 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Date Y.Nakazato M.Yamaguchi H.Kangawa K.Kinoshita Y.Chiba T.Ueta Y.Yamashita H.Matsukura S: "Enterochromaffin-like cell, a cellular Source of uroguanylin in rat stomach." Endocrinology, in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Chino N.Kubo S.Kitani T.Yoshida T, Tanabe R, Kobayashi Y.Nakazato M, Kangawa K, Sakakibara S: "Topological isomers of human uroguanylin : Interconversion between biologically active and inactive isomers." FEBS Lett. 421. 27-31 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinoshita H, Nakazato M, Yamaguchi H, Matsukura S, Fujimoto S, Eto T: "Increased plasma guanylin levels in patients with impaired renal function." Clin Nephrol. 47. 28-32 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyazato M, Nakazato M, Matsukura S, Kangawa K, Matsuo H: "Genomic structure and chromosomal localization of human uroguanylin gene." Genomics. 43. 359-365 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinoshita H, Fujimoto S, Nakazato M, Yokota N, Date Y, Yamaguchi H, Hisanaga S, Eto T: "Plasma and urine levels of uroguanylin and its molecular forms in renal diseases." Kidney Int. 52. 1028-1034 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中里雅光: "最新内科学大系プログレス2、内分泌・代謝疾患" グアニリンファミリーペプチド, 42-45 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中里雅光: "消化管ホルモン" 水・NaCI代謝調節に作用する新しい消化管ペプチドグアニリンファミリー,

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi