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Homeostasis and its pathology of cellular lipid

Research Project

Project/Area Number 10044309
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionNagoya City University

Principal Investigator

YOKOYAMA Shinji  Nagoya City University Medical School professor, 医学部, 教授 (10142192)

Co-Investigator(Kenkyū-buntansha) TSUJITA Maki  Nagoya City University Medical School research associate, 医学部, 助手 (10253262)
DOHMAE Sumiko (ABE Sumiko)  Nagoya City University Medical School assistant professor, 医学部, 講師 (70227700)
ITO Junichi  Nagoya City University Medical School associate professor, 医学部, 助教授 (60167260)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1999: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1998: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordscholesterol / phosphlipid / lipoprotein / apolipoprotein / 細胞 / 脂質 / リポタンパク質 / アポリポタンパク質
Research Abstract

We studied the HDL assembly system mediated by apolipoprotein-cell interaction by using three model systems.
1) Study on the apolipoprotein interaction site in mouse leukemic cell line cells RAW 264. A kinase must be activated for this cell to express the binding site with apolipoprotein and to gegerate HDL with cellular lipid. It takes several hours for the full expression which is suppressed by various metabolic inhibitors for protein synthesis. Therefore, contribution of a membrane protein(s) is suggested. Interestingly, there was no apparent difference in expression of ABC1 which has been shown as a mutation site in familial HDL deficiencies.
2) Study on intracellular cholesterol trafficking linked to the HDL assembly system in human leukemic cell line cells THP-1. While THP-1 cells gegerate HDL only with cellular phospholipid by apolipoprotein before differentiation, HDL is enriched with cholesterol after PMA induces differentiation. Both ABC1 and caveolin-1 are induced by PMA, and the antisense DNA selectively reduces cholesterol content in the HDL generated. Therefore, caveolin-1 is responsible for incorporation of cholesterol into the HDL.
3) Study on physiological relevance of the HDL assembly system in a mouse model by using an inhibitor of the apolipoprotein-cell interaction, probucol. Mouse HDL is rapidly and remarkably decreased by probucol, while no significant change is demonstrated in the massages of HDL-relating proteins including ABC1 and in the HDL clearance. The HDL assembly by apolipoprotein and the peritoneal macrophage is markedly decreased by probucol, showing that this is a major source of plasma HDL in mice.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 横山信治: "Apolipoprotein-mediated cellular cholesterol efflux"Biochimica et Biophysica Acta. 1392. 1-15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 笹井冠奈 横山信治 他: "Human cholesteryl ester transfer protein measured by enzyme-linked immunsorbent assay with two monoclonal antibodies"Clinical Chemistry. 44. 1466-1473 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] リサ・メイン 横山信治 他: "Cholesteryl Ester Transfer Protein Reaction between Plasma Lipoproteins"Journal of Biochemistry. 124. 237-243 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 伊藤仁一 横山信治 他: "Differential generation of High-Density Lipoprotein by Endgenous and Exogenous Apolipoproteins in Cultured Fetal Rat Astrocytes"Journal of Neurochemistry. 72. 2362-2369 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 斎藤和典 横山信治 他: "Epitope mapping for the anti-rabbit cholesteryl ester transfer protein antibody"Journal of Lipid Research. 40. 2013-2021 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinji Yokoyama: "Apolipoprotein-mediated cellular cholesterol efflux"Biochimica et Biophysica Acta. 1392. 1-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanna Sasai et-al: "Human choleteryl ester transfer protein measured by enzyme-linked immunosorbent assay with two monoclonal antibodies"Clinical Chemistry. 44. 1466-1473 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lisa A. Main et-al: "Cholesteryl Ester Transfer Protein Reaction between Plasma Lipoproteins"Journal of Biochemistry. 124. 237-243 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jin-ichi Ito et-al: "Differential Generation of High-Density Lipoprotein by Endogenous and Exogenous Apolipoproteins in Cultured Fetal Rat Astrocytes"Journal of Neuro chemistry. 72. 2362-2369 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kazunori Saito et-al: "Epitope mapping for the anti-rabbit cholesteryl ester transfer protein antibody"Journal of Lipid Research. 40. 2013-2021 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 横山伸治: "Apolipoprotein-mediated cellular cholesterol efflux"Biochimica et Biophysica Acta. 1392. 1-15 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 笹井冠奈 横山信治 他: "Human cholesteryl ester protein measured by enzyme-linked immunosorbent assay with two monoclonal antibodies"Clinical Chemistry. 44. 1466-1473 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] リサ・メイン 横山信治 他: "Cholesteryl Ester Transfer Protein Reaction between Plasma Lipoproteins"Journal of Biochemistry. 124. 237-243 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 伊藤仁一 横山信治 他: "Differential Generation of High-Density Lipoprotein by Endogenous and Exogenous Apolipoproteins in Cultured Fetal Rat Astrocytes"Journal of Neurochemistry. 72. 2362-2369 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 斎藤和典 横山信治 他: "Epitope mapping for the anti-rabbit cholesteryl ester transfer protein antibody"Journal of Lipid Reasearch. 40. 2013-2021 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shinji Yokoyama.: "Apolipoprotein-Mediated Cellular Cholesterol Efflux(review)." Biochim.Biophys.Acta. 1392. 1-15 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanna Sasai et-al.: "Human cholesteryl ester transfer protein(CETP)measured by enzyme-linked immunosorbent assay with two monoclonal antibodies against rabbit CETP:" Clinical Chemistry. 44. 1466-1473 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Lisa A.Main et-al.: "Cholesteryl ester transfer protein reaction between plasma lipoproteins." J.Biochemistry. 124. 237-243 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jini-ichi Ito,Li-Yong Zhang,Michiyo Asai and Shinji Y.: "Defferential generation of high density lipoprotein by endogenous and exogenous apolipoproteins in cultured fetal rat astrocytes." J.Neurochem.(in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2021-11-05  

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