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Crosstalk of immune and nervous systems by 3-D imaging

Research Project

Project/Area Number 10044312
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNagoya City University

Principal Investigator

NAKANISHI Mamoru  Nagoya City University Faculty of Pharmaceutical Sciences Professor, 薬学部, 教授 (90090472)

Co-Investigator(Kenkyū-buntansha) TESHIMA Reiko  National Institute of Health Sciences Principal Investigator, 主任研究官 (50132882)
FURUNO Tadahide  Nagoya City University Faculty of Pharmaceutical Sciences Assistant Professor, 薬学部, 講師 (80254308)
ビーネンストック ジョン  マクマスター大学, 健康科学部, 教授
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsAllergy / Mast cells / Bradykinin / Confocal laser scanning microscopy / Calcium ion / NK-1 antagonist / Substance P / Nerves / サブスタンスP / 好塩基球 / 共焦点レーザ顕微鏡 / アンタゴニスト
Research Abstract

Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. However, whether mast cell activation occurs as a direct response to neuronal activation or requires an intermediary cell is unclear. Addressing this issue, we used an in vitro coculture approach comprising cultured murine superior cervical ganglia and rat leukemia basophilic cells (RBLs ; possesses properties of mucosal-type mast cells). Following loading with the calcium fluorophore, Fluo-3, neurite-RBL units (separated by <50nm) were examined by confocal laser scanning microscopy. Addition of bradykinin, or scorpion venom, dose-dependently elicited neurite activation (i.e., CaィイD12+ィエD1 mobilization) and, after a lag period, RBL CaィイD12+ィエD1 mobilization. Neither bradykinin nor scorpion venom had any direct effect on the RBLs in the absence of neurites. Addition of a neutralizing substance P Ab or a neurokinin (NK)-1 receptor antagonist, but not an NK-2 receptor antagonist, dose-dependently prevented the RBL activation that resulted as a consequence of neural activation by either bradykinin or scorpion venom. These data illustrate that nerve-mast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, is an important mediator of this communication. Our findings have implications for the neuroimmune signaling cascades that are likely to occur during airways inflammation, intestinal hypersensitivity, and other conditions in which mast cells feature.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Nakamura, R., Nakanishi, M.: "Atomic force microscopy to study the effectsof ITIM-bearing FcγRIIB on the activation of RBl-2H3 cells"Immunol. Lett.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Teshima, R. and Nakanishi, M.: "Cell signaling and imaging in mast cell activation"Curr. Trends. Immunol.. 2. 173-186 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, R. et al.: "Direct neurite-mast cell (RBL) communication in vitro occurs via the neuropeptide substance P"J. Immunol.. 163. 2410-2415 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Teshima, R. et al.: "Degranulation in mast cells and basophils-Confocal and atomicforce microscopy-"Bioimages. 7. 97-103 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura, R. and Nakanishi, M.: "Atomic force microscopy to study the degranulaiton in rat peritoneal mast cell activation"Immunol. Lett.. 69. 307-310 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tenjinbaru, K. et al.: "Nuclear translocation of gree fluoescent protein-nuclear factor κB with a distinct lag time in living cells"FEBS Lett.. 404. 1-4 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中西 守 他: "バイオイメージングの最先端"先端医療技術研究所. 324 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Nakamura: "Atomic force microscopy to study the effects of ITIM-bearing FcγRIIB on the activation of RBL-2H cells."Immunol. Lett.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Teshima: "Cell signaling and imaging in mast cell activation."Curr. Trends. Immunol.. 2. 173-186 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Suzuki: "Direct neurite-mast cell (RBL) communication in vitro occurs via the neuropeptide substance P."J. Immunol.. 163. 2410-2415 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Teshima: "Degranulation in mast cells and basophils -Confocal and atomic force microscopy-."Bioimages. 7. 97-103 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Nakamura: "Atomic force microscopy to study the degranulation in rat peritoneal mast cell activation."Immunol. Lett.. 69. 307-310 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Tenjinbaru: "Nuclear translocation of green fluorescent protein-nuclear factor κB with a distinct lag time in living cells."FEBS Lett.. 404. 1-4 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura,R, Nakanishi,M.: "Atomic force microscopy to study the effects of ITIM-bearing FcyRIIB on the activation of RBL-2H3 cells"Immunol.Lett.. in press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Teshima,R,and Nakanishi,M.: "Cell signaling imaging in mast cell activation"Curr.Trends.Immunol.. 2. 173-186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,R.et al.: "Direct neurite-mast cell(RBL)communication in vitro occurs via the neuropeptide substance P"J.Immunol.. 163. 2410-2415 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Teshima,R.et al.: "Degranulation in mast cells and basophils-Confocal and atomic force microscopy-"Bioimages. 7. 97-103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura,R,and Nakanishi,M: "Atomic force microscopy to study the degranulation in rat peritoneal mast cell activation"Immunol.Lett.. 69. 307-310 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tenjinbaru,K. et al.: "Nuclear translocation of green fluorescent protein-nuclear factor κB with a distinct lag time in living cells"FEBS Lett.. 404. 1-4 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中西 守 他: "バイオイメージングの最先端"先端医療技術研究所. 324 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] C.Kawaura: "Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative." FEBS Lett.421. 69-72 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Noguchi: "Membrance fusion plays an important role in gene transfection mediated by cationic liposomes." FEBS Lett.433. 169-173 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Nakamura: "Atomic force microscopy for the cellular localization of secretory granules and coated pits in rat basophilic leukemia cells after antigen stimulation." Bioimages. 6. 69-75 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Tenjinbaru: "Nuclear translocation of green fluorescent protein-nuclear factor κB with a distinct lag time in living cells." FEBS Lett.444. 1-4 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Nakanishi: "Dynamic structures of CD63 antigen with a green fluorescent protein in RBL-2H3 cells after stimulation with antigen." Proceedings 10th International Congress of Immunology. 4(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Nakanishi: "Cell Signaling and Imaging in Mast Cell Activation" Current Trends in Immunology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中西 守: "見る技術" 共立出版, 350 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中西 守: "生物工学基礎コース (分析.計測法)" 丸善, 187 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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