| Project/Area Number |
10044317
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Saitama Medical School |
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Principal Investigator |
HIRAYAMA Kenji Professor, Dept of Medical Zoology, Saitama Medical School, 医学部, 教授 (60189868)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Somei Professor, Dept of Parasitol., Inst of Medical Science, University of Tokyo, 医科学研究所, 教授 (00009622)
張 紹基 中国江西省寄生虫病研究所, 教授
袁 鴻昌 中国上海医科大学, 医学部, 教授
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Schistosoma japonicum / re-infection study / HLA / cDNA library / China / Protective immunity / mass-treatment / liver fibrosis / 日本住血吸虫症 / 高リスク集団 / 遺伝 |
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| Research Abstract |
1)Re-infection study was re-challenged in the new target village, Caipeng, in Jiangxi.
180 persons (29.2% of the total examined) in the village were fecal egg positive in December 1998. All the patients were treated in March, 1999 and re-examined for their re-infection in December, 1999. Almost 60 persons were re-infected. Those susceptible persons will be analyzed for their HLA types. We have already found in Zhuxi that there was a special resistant HLA-DR type present. We will see if this is reproducible the new village.
2)Immunogenetic analysis of the post-schistosomal liver fibrosis.
To identify the responsible antigen that will stimulate or modulate the fibrotic repose to eggs, we screened recombinant antigens from an expression library of eggs using positive human serum. 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The deduced amino acid sequence had no binding motif of resistant HLA-DRB1*11O1 but had a motif of susceptible HLA-DRB1* 1501. The lymphocyte study should be done to see their real reactivity to this recombinant antigen.
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