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Molecular Basis for Neurohormonal Control of Salmonid Spawning Migration

Research Project

Project/Area Number 10102001
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionGrant-in Aid for Specially Promoted Research (2)

Principal Investigator

URANO Akihisa  Hokkaido Univ., Grad.Sch.of Sci., Prof., 大学院・理学研究科, 教授 (00142232)

Co-Investigator(Kenkyū-buntansha) ANDO Hironori  Hokkaido Univ., Grad.Sch.of Sci., Assist.Prof., 大学院・理学研究科, 助手 (60221743)
ITO Etsuro  Hokkaido Univ., Grad.Sch.of Sci., Assoc.Prof., 大学院・理学研究科, 助教授 (80203131)
ABE Shuiti  Hokkaido Univ., Fac.of Sci., Assoc.Prof., 理学部, 助教授 (80125278)
鈴木 範男  北海道大学, 大学院・理学研究科, 教授 (20082133)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥117,000,000 (Direct Cost: ¥117,000,000)
Fiscal Year 2000: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 1999: ¥39,000,000 (Direct Cost: ¥39,000,000)
Fiscal Year 1998: ¥48,000,000 (Direct Cost: ¥48,000,000)
KeywordsSalmon / Migration / Neurohormone / GnRH / Vasotocin / Genetic Program / Instinctive Behavior / サケ科魚類 / 視床下部 / 下垂体 / 産卵回遊 / 母川回帰 / 神経葉ホルモン / 下垂体ホルモン / ステロイドホルモン / ミトコンドリアDNA / グアニル酸シクラーゼ
Research Abstract

Spawning migration of salmon is a typical stereotyped or instinctive behavior which is genetically programmed. However, molecular approaches are rare to reveal what is a genetic program of salmon migration. Aiming clarification of such genetic program as the final goal, we examined molecular bases of neuroendocrine mechanisms for salmon spawning migration. Since spawning migration is reproductive behavior, we hypothesized that preoptic neurons producing gonadotropin-releasing hormone (GnRH) and vasotocin (VT) have a crucial role in coordination of the sensory, motor and motivational function of the central nervous system and the neuroendocrine function of the brain-pituitary-gonadal axis. The coordinated activity of the central nervous system and the neuroendocrine system should be crucial for the success of reproduction, that is, the neural controls of migratory and spawning behavior must be temporally and spacially coincident. Herewith. we determined absolute changes in the amounts of mRNAs encoding neurohormones and pituitary hormones along migratory pathways to elucidate changes in expression of hormonal genes during migration. We further analyzed regulatory mechanisms of expression of hormonal genes in salmonids. The results obtained through the present project actually support our hypothesis mentioned above.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Taniyama,S.: "Effects of GnRH analogue on expression of genes encoding GH/PRL/SL family and Pit-1/GHF-1 in the pituitaries of pre-spawning sockeve salmon"General and Comparative Endocrinology. 118(3). 418-424 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Salam,MD.A.: "Immunohistochemical analysis of pituitary cells in pre-spawning chum salmon."Zoological Sciences. 17(6). 805-819 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Higa,M.: "Quantitative analysms of fushi tarazu factor l homolog mRNAs in the pituitary of salmon at different pre-spawning stages."Biology of Reproduction. 63. 1756-1763 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitahashi,T.: "Micro data logger analyses of homing behavior of chum salmon in Ishikari bay."Zoological Sciences. 17(9). 1247-1253 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato,S.: "Genetic variations among Japanese populations of chum salmon inferred from the nucleotide sequences of the mitochondrial DNA control region."Zoological Science. 18(6). 99-106 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito,D.: "Effects of sexual maturation and osmoregulatory capacity on expression of vasotocin gene in pre-spawning chum salmon."Zoological Sciences. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ando,H.: "Signal transduction pathways and transcription factors in the gonadotropin-releasing hormone-stimulated gonadotropin subunit gene expression"Comparative Biochemistry and Physiology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Higa,M.: "Expression of Pit-1 and fushi-tarazu factor 1 homolog genes in the pituitary of pre-spawning chum and sockege salmons"Comparative Biochemistry and Physiology. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 浦野明央: "行動の分子生物学 : サケの回遊行動を支配するホルモンと遺伝子"シュプリンガー・フェアラーク東京. 162 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Taniyama, S., Kitahashi, T., Ando, H., Kaeriyama, M., Zohar, Y., Ueda, H.and Urano, A.: "Effects of GnRH analogue on expression of genes encoding GH/PRL/SL family and Pit-1/GHF-1 in the pituitaries of pre-spawning sockeye salmon."General and Comparative Endocrinology. 118(3). 418-424 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Salam, MD.A., Ando, H., Ban, M., Ueda, H.and Urano, A.: "Immunohistochemical analysis of pituitary cells in pre-spawning chum salmon."Zoological Science. 17(6). 805-819 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Higa, M., Kanda, H., Kitahashi, T., Ito, M., Shiba, T.and Ando, H.: "Quantitative analysis of fushi tarazu factor 1 homolog mRNAs in the pituitary of salmon at different pre-spawning stages."Biology of Reproduction. 63. 1756-1763 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitahashi, T., Ando, H., Urano, A., Ban, M., Saito, S., Tanaka, H., Naito, Y.and Ueda, H.: "Micro data logger analyses of homing behavior of chum salmon in Ishikari bay."Zoological Science. 17(9). 1247-1253 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato, S., Ando, J., Ando, H., Urawa, S., Urano, A.and Abe, S.: "Genetic variations among Japanese populations of chum salmon inferred from the nucleotide sequences of the mitochondrial DNA control region."Zoological Science. 18(1). 99-106 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito, D., Ota, Y., Hiraoka, S., Hyodo, S., Ando, H.and Urano, A.: "Effects of sexual maturation and osmoregulatory capacity on expression of vasotocin gene in pre-spawning chum salmon."Zoological Science. 18(in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ando, H., Hew, C.L.and Urano, A.: "Signal transduction pathways and transcription factors involved in the gonadotropin-releasing hormone-stimulated gonadotropin subunit gene expression."Comparative Biochemistry and Physiology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Higa, M., Ando, H.and Urano, A.: "Expression of fushi tarazu factor 1 homolog and Pit-1 genes in the pituitaries of pre-spawning chum and sockeye salmon."Comparative Biochemistry and Physiology. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Taniyama,S.: "Effects of GnRH analogue on expression of genes encoding GH/PRL/SL family and Pit-1/GHF-1 in the pituitaries of pre-spawning sockeye salmon"General and Comparative Endocrinology. 118(3). 418-424 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Salam,MD.A.: "Immunohistochemical analysis of pituitary cells in pre-spawning chum salmon."Zoological Sciences. 17(6). 805-819 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Higa,M.: "Quantitative analysis of fushi tarazu factor 1 homolog mRNAs in the pituitary of salmon at different pre-spawning stages."Biology of Reproduction. 63. 1756-1763 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitahashi,T.: "Micro data logger analyses of homing behavior of chum salmon in Ishikari bay."Zoological Sciences. 17(9). 1247-1253 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato,S.: "Genetic variations among Japanese populations of chum salmon inferred from the nucleotide sequences of the mitochondrial DNA control region."Zoological Science. 18(6). 99-106 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Saito,D.: "Effects of sexual maturation and osmoregulatory capacity on expression of vasotocin gene in pre-spawning chum salmon."Zoological Sciences. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ando,H.: "Signal transduction pathways and transcription factors in the gonadotropin- releasing hormone-stimulated gonadotropin subunit gene expression"Comparative Biochemistry and Physiology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Higa,M.: "Expression of Pit-1 and fushi tarazu factor 1 homolog genes in the pituitary of pre-spawning chum and sockeye salmons."Comparative Biochemistry and Physiology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浦野明央: "行動の分子生物学:サケの回遊行動を支配するホルモンと遺伝子"シュプリンガー・フェアラーク東京. 162 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ando H.: "Expression of salmon corticotropin-releasing hormone precursor gene in the preoptic nucleus in stressed rainbow trout"General and Comparative Endocrinology. 113(1). 87-95 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ota Y.: "Seasonal changes in expression of neurohypophysial hormone genes in the preoptic nucleus of immature female masu salmon"General and Comparative Endocrinology. 116(1). 31-39 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ota Y.: "Differences in seasonal expression of neurohypophysial hormone genes in ordinary and precocious male masu salmon"General and Comparative Endocrinology. 116(1). 40-48 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taniyama S.: "Changes in the levels of mRNAs for GH/PRL/SL family and Pit-1/GHF-1 in the pituitaries of pre-spawning chum salmon"Journal of Molecular Endocrinology. 23. 189-198 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Salam, Md.A.: "High population density decreased the number and sizes of pituitary growth hormone cells in juvenile chum salmon"Zoological Science. 16(6). 945-954 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taniyama S.: "Effects of GnRH analogue on expression of genes encoding GH/PRL/SL family and Pit-1/GHF-1 in the pituitaries of pre-spawning sockeye salmon"General and Comparative Endocrinology. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ando,H.: "Localization of mRNA encoding thyrotropin-releasing hormone precursor in the brain of sockeye salmon." Zoological Science. 15(6). 953-961 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitahashi, T.: "Changes in the levels of gonadotropin subunit mRNAs in the pituitary of pre-spawning chum salmon" Zoological Science. 15(5). 753-760 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitahashi, T.: "GnRH analog stimulates gonadotropin II gene expression in maturing sockeye salmon." Zoological Science. 15(5). 761-765 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitahashi, T.: "Gonadoropin-releasing hormone analog and sex steroids shorten homing duration of sockeye salmon in Lake Shikotsu." Zoological Science. 15(5). 767-771 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mikami, T.: "Molecular cloning of cDNAs and expression of mRNAs encoding alpha and beta subunits of soluble guanylyl cyclase from medaka fish Oryzias latipes." European Journal of Biochemistry. 253. 42-48 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sadamoto, H.: "Histochemical study on the relation between NO- generative neurons and central circuitry for feeding in the pond snail Lymnaea stagnalis." Neuroscience Research. 32. 57-63 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2018-03-28  

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