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光合成関連遺伝子群の体系的同定

Research Project

Project/Area Number 10168210
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionNagoya University

Principal Investigator

杉浦 昌弘  名古屋大学, 遺伝子実験施設, 教授 (80027044)

Co-Investigator(Kenkyū-buntansha) 杉田 護  名古屋大学, 人間情報学研究科, 教授 (70154474)
Project Period (FY) 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsラン藻 / ゲノム / 整列クローン / 塩基配列 / Anacystis nidulans
Research Abstract

1. Anacystis nidularns 6301ゲノム解析:
ゲノムDNAのBam HI部分切断片とλDashIIで作製した全ゲノミック・ライブラリーを物理地図にそって各断片ごとにサブ・ライブラリー化し、クローンの整列化の90%を完了した。
整列化の済んだλクローン約600個の両端の塩基配列を決定した(各800bpで合計0.48Mb)
2. 情報公開:
ホームページ(www.bio.nagoya-u.ac.jp:8001/^gene)を作製し、Anacystisゲノムのλクローン整列化の進行状況を逐一公開している。また、この株は、国内外とも最もよく使用されているため、他のグループで決定された塩基配列をまとめて公開を始めた(171件、約200kb)

Report

(1 results)
  • 1998 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Watanabe,T.,M.Sugita,M.Sugiura: "Identification of 10Sa RNA(tmRNA)homologues from the cyanobacterium Synechococcus sp.strain PCC6301 and related organisms." Biochim.Biophy.Acta. 1396. 97-104 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyagi,T.,S.Kapoor,M.Sugita and M.Sugiura: "Transcript analysis of tobacco plastid rps2/atnI/H/F/A operon reveals the existen-ce of a non-consensus type(NCII)promoter upstream to atpI coding sequence." Mol.Gen.Genet.257. 299-307 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akama.K,Y.Yukawa,M.Sugiura and I.Small: "Plant cytosolic tRNA^<His> possesses an exceptional C_<54> in the canonical TΨC loop." Nucl.Acids.Res.26. 2708-2714 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirose,T.,T.Kusumegi and M.Sugiura: "Translation of tobacco chloroplast rps14 mRNA depends on a Shine-Dalgarno-like sequence in the 5'-untranslated region but not on internal RNA editing in the coding region." FEBS Lett.430. 257-260 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiura,M.,T.Hirose and M.Sugita: "Evolution and mechanism of translation in chloroplasts." Annu.Rev.Genetics. 32. 437-459 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiura,M.: "From plastid to cyanobacterial genomes." In The Phototrophic Prokaryotes. 印刷中 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiura,M.: "The discovery of the complete sequence of tobacco and rice chloroplast genomes.In Discoveries in Plant Biology" World Scientific Publishing Co.Ltd,Hong Kong, 45-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiura,M.: "Phytogene sequence:From cyanobacteia to plastids.In From Symbiosis to Eukaryotism-Endocytobiology VII" Univ Geneva Press,in press(印刷中), (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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