• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles of protein-protein interactions in nuclear signal transductions

Research Project

Project/Area Number 10179102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionYokohama City University (2001)
Nara Institute of Science and Technology (1998-2000)

Principal Investigator

SHIRAKAWA Masahiro  Yokohama City University, professor, 大学院・総合理学研究科, 教授 (00202119)

Co-Investigator(Kenkyū-buntansha) HORIKOSHI Masami  Tokyo University, Associate Professor, 分子細胞生物学研究所, 助教授 (70242089)
FUJITA Takashi  The Tokyo Metropolitan Institute of Medical Science, Group Leader, 室長 (10156870)
NISHIMURA Yoshibumi  Yokohama City University, professor, 大学院・総合理学研究科, 教授 (70107390)
KATAHIRA Masato  Yokohama National University, Associate Professor, 大学院・環境情報研究院, 助教授 (70211844)
NAKAJIMA Shinsuke  Osaka University, Research Associate, 蛋白質研究所, 助手 (60324852)
中村 春木  大阪大学, 蛋白質研究所, 教授 (80134485)
松尾 浩  大阪大学, 蛋白質研究所, 助手 (60304052)
梅園 和彦  京都大学, ウイルス研究所, 教授 (50183752)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥113,200,000 (Direct Cost: ¥113,200,000)
Fiscal Year 2001: ¥26,400,000 (Direct Cost: ¥26,400,000)
Fiscal Year 2000: ¥29,700,000 (Direct Cost: ¥29,700,000)
Fiscal Year 1999: ¥27,400,000 (Direct Cost: ¥27,400,000)
Fiscal Year 1998: ¥29,700,000 (Direct Cost: ¥29,700,000)
Keywordstranscription / protein structure / chromatin / NMR / X-ray crystallography / X線結晶構造解析 / 転写 / 蛋白質 / 立体構造 / 遺伝子修復 / NMR / 基本転写因子 / クロマチン / DNA結 / G蛋白質共役受容体 / 水素結合 / X線 / 蛋白質-蛋白質相互作用 / 蛋白質の立体構造
Research Abstract

Through close collaborations between members of this grant group, many new findings in the field of transcription, DNA repair and chromatin remodeling were reported. One of them is the structure of the complex between the methyl-CpG-binding domain of MBD1 and a methylated DNA, by Shirakawa and co-workers. CpG methylation in vertebrates is important for the regulation of gene activity, genomic stability and chromatin structure ; differences in the DNA-methylation status are associated with imprinting phenomena, development, and carcinogenesis. Methylation signals are interpreted by protein factors that contain methyl-CpG-binding domains (MBDs). Shirakawa and co-wokers have determined solution structure of the MBD of the human methylation-dependent transcriptional regulator MBD1 bound to a methylated DNA. The methyl groups at the methylation site are recognized through extensive hydrophobic contacts with aliphatic and aromatic portions of arginine, tyrosine, and serine residues that are totally conserved among the MBD family. Discrimination of the CG sequence is due to the sama arginine and tyrosine residues. The structure indicates how MBD may access to nucleosomal DNA without encountering steric interference from core histones. It also suggests that some residues of MeCP2 that are mutated in Rett syndrome are located at the protein-DNA interface, providing a structural basis to understand the consequence of these mutations.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Ohki, I.: "Solution Structure of the Methyl-CpG-binding Domain of Human MBD1 in Complex with Methylated DNA"Cell. 105. 487-497 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jee, J-G.: "Solution Structure of the Fibronectin Type III Domain from Bacillus circulans WL-12 Chitinase A1"Journal of Biological Chemistry. 277. 1388-1397 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Inooka, H.: "Conformation of a peptide ligand bound to its G-protein coupled receptor"Nature Structural Biology. 8. 161-165 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jee, J. -G., Ikegami, T., Hashimoto, M., Kawabata, T., Ikeguchi, M., Watanabe, T., and Shirakawa, M.: "Solution Structure of the Fibronectin Type III Domain from Bacillus circulans WL-12 Chitinase A1"Journal of Biological Chemistry. 277. 1388-1397 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohki, I., Shimotake, N., Fujita, N, Jee, J. -G., Ikegami, T., Nakao, M., and Shirakawa, M.: "Solution Structure of the Methyl-CpG-binding Domain of Human MBD1 in Complex with Methylated DNA"Cell. 105. 487-497 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Inooka, H., Ohtaki, T., Kitahara, O., Ikegami, T., Endo, S., Kitada, C., Ogi, K., Onda, H., Fujino, M., and Shirakawa, M.: "Conformation of a peptide ligand bound to its G-protein coupled receptor"Nature Structural Biology. 8. 161-165 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi, M., Moruboeuf, F., Sakai, Y.他: "Role of tryptophan resideus in recognition of mutagenic oxdized nucleotides by human antimutator MTH1 protein"Journal of Molecular Biology. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakai, Y., Furuichi, M., Takahashi, M.他: "A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-Oxo-dGTP by human MTH1"Journal of Biological Chemistry. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Kouno, T., Miura, K., Kanematsu, T.他: "1H,13C and 15N resonance assignments of GABARAP,GABAA receptor associated protein"J.biomol.NMR. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Jee, J.-G., Ikegami, T., Hashimoto, M.他: "Solution Structure of the Fibronection TypeIII Domain from Bacillus circulars WL-12 Chitinase A1"Journal of Biological Chemistry. 277. 1388-1397

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohki, I., Shimotake, N., Fujita, N.他: "Solution Structure of the Methyl-CpG-binding Domain of Human MBD1 in Complex with Methylated DNA"Cell. 105. 487-497

    • Related Report
      2001 Annual Research Report
  • [Publications] H Inooka 他9名: "Conformation of a peptide ligand bound to its G-protein coupled receptor."Nature Structural Biology. 8. 161-165 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Ikgami 他5名: "Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli."Nature Structural Biology. 40. 375-386 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Morikawa,M.Shirakawa: "Three-dimensional structural views of damaged-DNA recognition : T4 endonuclease V, E. coli Vsr protein, and human nucleotide excision repair factor XPA"Mutation Research. 460. 257-275 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Fujita 他6名: "Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1."Molecular Cellular Biology. 20. 5107-5118 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Mishima 他5名: "Intermolecular ^<31>P-^<15>N and ^<31>P-^1H scalar couplings across hydrogen bonds formed between a protein and a nucleotide."J.Am.Chem.Soc.. 122. 5883-5884 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Ikegami 他5名: "Structure of the Chitin-Binding Domain of Bacillus circulans WL-12 Chitinase Al."Journal of Biological Chemistry. 275. 13654-13661 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Ikegami,T.Okada,M,hashimoto,M.Shirakawa その他(計6名;5番目): "Solution structure of the chitin-binding domain of Bacillus circulans WL-12 Chitinwe A1"Journal of Biological chemistry. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] I.Ohki,N.Shimotake,M.Shirakawa その他(計5名;5番目): "Solution stracuture of the methyl-CpG-binding domain of Human MBD1"The EMBO Journal. 18. 6653-6661 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.kabe,N.Goto,その他(計9名;7番目): "The role of human MBF1 as a transcriptional ***********"Journal of Biological Chemistry. 274. 34196-34202 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Mishima,J.Ozaki, その他(計9名;9番目): "Research-assignments,secondary structure add on relaxing data of the human transcriptional ********** hMBF1(57-148)"Journal of biomolecular NMR. 14. 373-376 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Ozaki,T.Ikegami,その他(計9名;9番目): "Identification of the core domain and the secondary structure of the transcriptional ********** MBF1"Genes to Cells. 4. 415-424 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikegami, T その他: "Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA" Nature Structural Biology. 5. 701-706 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikawa, T その他: "Solution structure of the DNA-binding domain of human telomeric protein, hTRf1" Structure. 6. 1057-1065 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoneyama, M.その他: "Direct Triggentry of the Type I Interferon System by virus infection Activation of a transcription factor complex containing IRF-3 and CBP1030" The EMBO Journal. 17. 1087-1095 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi, M その他: "I dostification of a photorecopter cell-Specific nuclear recepter" Proc.Natl.Acad.Sci.USA. (in press).

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi