| Project/Area Number |
10182101
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas (A)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
OKADA Kiyotaka Kyoto Univ., Grad.Sch.Science, Professor, 大学院・理学研究科, 教授 (50101093)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Hiroo Univ. Tokyo, Grad.Sch.Science, Professor, 大学院・理学系研究科, 教授 (10165293)
MATUOKA Makoto Nagoya Univ., Bioscience Center, Professor, 生物分子応答研究センター, 教授 (00270992)
SUGIYAMA Munetaka Univ. Tokyo, Grad.Sch.Science, Assoc. Professor, 大学院・理学系研究科, 講師 (50202130)
ARAKI Takashi Kyoto Univ., Grad.Sch.Science, Assoc. Professor, 大学院・理学研究科, 助教授 (00273433)
SHINOZAKI Kazuo Riken Institures, Lab. Plant Molec. Biol., Professor, 理化学研究所, 主任研究員 (20124216)
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| Project Period (FY) |
1998 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥309,800,000 (Direct Cost: ¥309,800,000)
Fiscal Year 2001: ¥76,800,000 (Direct Cost: ¥76,800,000)
Fiscal Year 2000: ¥77,600,000 (Direct Cost: ¥77,600,000)
Fiscal Year 1999: ¥78,000,000 (Direct Cost: ¥78,000,000)
Fiscal Year 1998: ¥77,400,000 (Direct Cost: ¥77,400,000)
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| Keywords | EMBRYO GENESIS / MERISTEM / REGULATORY GENE / PLANT ORGANOGENESIS / ARABIDOPSIS / ORYZA / ZINNIA / MUTANT |
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| Research Abstract |
This project aimed to understand the genetic regulatory systems which contribute to make up multi-cellular body of plants, especially in the embryo development and in the meristem formation and maintenance. The major results are as follows.
1. Okada group : Analysis of a root meristem-defective mutant, hlr, showed that it has a mutation in a subunit of proteosome complex. This is a novel observation showing that programmed protein degradation is required for maintaining structure and function of the root meristem. This group also examined FTR gene controlling polarized cell elongation, CPC gene controlling epidermal cell specification, KOM gene controlling male gametophyte, and DAD1 gene controlling jasmonic acid synthesis and anther opening. 2. Araki group studied FAS1 and 2 genes responding to the formation of shoot apical meristem (SAM). The genes encode subunits of chromatin assembly factor (CAF). The group also analyzed a key flowering gene, FT, and identified new genes suppressing
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or enhancing the effect of overexpressing FT. 3. Kakimoto group identified isopentenyl transferases are the key enzyme in the cytokinin biosynthesis. The group also studied cytokinin receptors and their downstream signaling pathways. 4. Shinozaki group : The group constructed a large number of transposon-tagged Arabidopsis lines, and studied a set of mutants showing male sterile, embryo lethal, leaf malmorphology. 5. Sugiyama group studied a set of mutnats showing temperature-dependent defects in the shoot and root regeneration process from callus. They cloned one of the genes, SRD2, and shown the gene is related to the transcriptional activation of snRNAs. 6. Tasaka group examined gene regulatory network controlling SAM formation in embryogenesis. They studied CUC1 and 2 genes and shown they regulate other SAM genes such as STM and KNOX. 7. Fukuda group : By using synchronized cell culture system of Xinnia and the tip-procedure, a large number of genes were identified which are required for vascular cell formation from leaf cells. Genes required for cell autolysis were also cloned. 8. Matsuoka group studied a variety of rice embryo pattern mutants showing many shoots, no shoots, aberrant organ form, and abberant orientation of meristem. The studies above were reported in journals or at international or national symposiums. Less
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