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Structural Biology of Biological Machineries through X-ray Protein Crystallography Reinforced by Synchrotron Radiation

Research Project

Project/Area Number 10188101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo (1999-2003)
Nagaoka University of Technology (1998)

Principal Investigator

TANOKURA Masaru (1999-2003)  東京大学, 大学院・農学生命科学研究科, 教授 (60136786)

三井 幸雄 (1998)  長岡技術科学大学, 工学部, 教授 (40012637)

Co-Investigator(Kenkyū-buntansha) TSUKIHARA Tomitake  大阪大学, 蛋白質研究所, 教授 (00032277)
MIURA Kinichiro  (株)プロテイオス研究所, 代表取締役(研究職) (30000227)
EBASHI Setsuro  岡崎国立共同研究機構, 生理研, 名誉教授 (10009863)
TANAKA Isao  北海道大学, 大学院理学研究科, 教授 (70093052)
MIKI Kunio  京都大学, 大学院理学研究所, 教授 (10116105)
千田 俊哉  長岡技術科学大学, 工学部, 助手 (30272868)
豊島 近  東京大学, 分子細胞生物学研究所, 教授 (70172210)
田之倉 優  東京大学, 大学院・農学生命科学研究科, 教授 (60136786)
Project Period (FY) 1998 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥52,200,000 (Direct Cost: ¥52,200,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2001: ¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2000: ¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1999: ¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1998: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsBiological machineries / Synchrotron radiation / Structural biology / X-ray crystallography / Complex of biomacromolecules / Group of biomacromolecules / membrane proteins / X線結晶学 / 蛋白結晶学 / 超分子複合体 / 膜蛋白質
Research Abstract

生体高分子(蛋白質、核酸など)の複合体で、"生きている状態"を演出する最小限の機能(ポリペプチド鎖の合成、特定の有機物質の分解代謝、プロトンの汲み出し、等々)を担っているような機能単位を「生物マシーナリー」と呼ぶ。驚くべきことに、多くの「生物マシーナリー」はその巨大さ(分子量が数十万から数百万)にもかかわらず、結晶として取り出すことが出来る。そして、いまやシンクロトロン放射光の利用を初めとする数々の技術革新のおかげで、蛋白質結晶学(生体高分子のX線結晶構造解析の技術)は一時代前よりもはるかに強力になり、丸ごとの「生物マシーナリー」の立体構造を原子のレベルで解明することが十分可能となった。実際、我国からも顕著な業績がいくつも上がった。しかしシンクロトロン放射光を利用した新しい蛋白質結晶学に関しては、技術的に特に優位にあるにもかかわらず、我国のこの分野への寄与は、いまのところ、甚だ不十分である。そこで、本特定領域の設定によって、生化学者、分子生物学者と結晶学者との交流の場(フォーラム)を設定し、両者間の共同作業を飛躍的に推進すると同時に、これらの全ての多彩な研究者達によるシンクロトロン放射光の活用が飛躍的に容易となるような,状況を作り出すことを目的とした。
今年度はこれまで行ってきた本特定領域のまとめとしてまとめの冊子(報告書)を作成すると共に本総括班ではこれらの研究活動全体の総括を行った。またその一環として平成15年7月に東京大学でワークショップを行った。

Report

(7 results)
  • 2003 Annual Research Report
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (95 results)

All Other

All Publications (95 results)

  • [Publications] Iwasaki, W., Sasaki, H., Nakamura, A., Kohama, K., Tanokura, M.: "Metal-free and Ca^<2+>-bound structures of a multidomain EF-hand protein, CBP4O, from the lower eukaryote, Physarum polycephalum."Structure. 11. 75-85 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hatano, K., Kojima, M., Tanokura, M., Takahashi, K.: "Nuclear magnetic resonance studies on the pK_a values and interactions of ionizable groups in bromelain inhibitor VI from pineapple stem."Biol.Chem.. 384. 93-104 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ito, M., Kato, Y., Nagata, K., Oda, Y., Yamagoe, S., Suzuki, K., Tanokura, M.: "Expression, oxidative refolding and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds."Protein Express.Purif.. 27. 272-278 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katayama, H., Nagata, K., Ohira, T., Yumoto, F., Tanokura, M., Nagasawa, H.: "The solution structure of molt-inhibiting hormone from the kuruma prawn Marsupenaeus japonicus."J.Biol.Chem.. 278. 9620-9623 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, R., Nagata, K., Yumoto, F., Kawakami, M., Nemoto, N., Furutani, M., Adachi, K., Maruyama, T., Tanokura, M.: "Three-dimensional solution structure of an archaeal FKBP with a dual function of peptidyl prolyl cis-trans isomerase and chaperone-like activities."J.Mol.Biol.. 328. 1149-1160 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamagata, K., Sawano, Y., Tanokura, M., Kuwajima, K.: "Multiple parallel-pathway folding of proline-free Staphylococcal nuclease"J.Mol.Biol.. 332. 1143-1153 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田之倉優, 伊東孝祐: "ナノバイオテクノロジーの最前線(植田充美 監修)、X線結晶構造解析からみるナノバイオテクノロジー"シーエムシー出版. 11 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kato, Y., Ito, M., Kawai, K., Nagata, K., Tanokura, M.: "Determinants of ligand specificity in groups I and IV WW domains as studied by sufface plasmon resonance and model building"Journal of Biological Chemistry. 277. 10173-10177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwasaki, W., Sasaki, H., Nakamura, A., Kohama, K., Tanokura, M.: "Metal-free and Ca^<2+>-bound structures of a multidomain EF-hand protein, CBP40,from the lower eukaryote, Physarum polycephalum"Structure. 11・1. 75-85 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasutake, Y., Watanabe, S., Yao, M., Tanaka, Y., Fukunaga, N., Tanaka, I.: "Structural of the Monomeric Isocitrate Dehydrogenase : Evidence of a Protein Monomerization by a Domain Duplication"Structure. 10. 1637-1648 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ose, T., Watanabe, K., Mie, T., Honma, M.Watanabe, H., Yao, M., Oikawa, H., Tanaka, I.: "Insight into a natural Diels-Alder reaction from the structure of macrouhomate svnthase"Nature. 422. 185-189 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kita, A., Matsunaga, S., Takai, A., Kataiwa, H., Wakimoto, T., Fusetani, N., Isobe, M., Miki, K.: "Crystal Structure of the Complex between Calyculin A and the Catalytic Subunit of Protein Phosuhatase 1"Structure. 10. 715-724 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Unno, M., Mizushima, T., Morimoto, Y., Tomisugi, Y., Tanaka, K., Yasuoka, N., Tsukihara, T.: "The Structure of the Mammalian 20S Proteasome at 2.75 A Resolution"Structure. 10. 609-618 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato, Y. Ito, M., Kawai, K. Nagata, K. and Tanokura, M.: "Determinants of ligand specificity in groups 1 and IV WW domains as studied by surface plasmon resonance and model building"J. Biol. Chem.. 277 (12). 10173-10177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwasaki, W., Sasaki, H., Nakamura, A., Kohama K. and Tanokura, M.: "Metal-free and Ca^<2+> bound structures of a mutidomain EF-hand protein, CBP40, from the lower eukaryote, Physarum polycephalum"Structure. 11(1). 75-85 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasutake, Y., Watanabe, S., Yao, M., Takada, Y., Fukunaga, N. & Tanaka, I.: "Structural of the Monomeric Isocitrate Dehydrogenase : Evidence of a Protein Monomerization by a Domain Duplication"Structure. 10. 1637-1648 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ose, T., Watanabe, K., Mie, T., Honma, M., Watanabe, H., Yao, M., Oikawa, H. & Tanaka, I.,: "Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase"Nature. 422. 185-189 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kita, A., Matsunaga, S., Takai, A., Kataiwa, H., Wakimoto, T., Fusetani, N., Isobe, M. & .Miki, K.: "Crystal Structure of the Complex between Calyculin A and the Catalytic Subunit of Protein Phosphatase 1"Structure. 10. 715-724 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Unno, M., Mizushima, T., Morimoto, Y., Tomisugi, Y., Tanaka, K., Yasuoka, N. & Tsukihara, T.: "The Structure of the Mammalian 20S Proteasome at 2.75 A Resolution"Structure. 10. 609-618 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kato, Y., Ito, M., Kawai, K., Nagata, K., Tanokura, M.: "Determinants of ligand specificity in groups I and IV WW domains as studied by surface plasmon resonance and model building"Journal of Biological Chemistry. 277・12. 10173-10177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sawano, Y., Muramatsu, T., Hatano, K., Nagata, K., Tanokura, M.: "Characterization of genomic sequence coding for bromelain inhibitors in pineapple and expression of its recombinant isoform"Journal of Biological Chemistry. 277・31. 28222-28227 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwasaki, W., Sasaki, H., Nakamura, A., Kohama K., Tanokura, M.: "Metal-free and Ca^<2+>-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote, Physarum polycephalum"Structure. 11・1. 75-85 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hatano, K., Kojima, M., Tanokura, M., Takahashi, K.: "Nuclear magnetic resonance studies on the pΚ_a values and interactions of ionizable groups in bromelain inhibitor VI from pineapple stem"Biological Chemistry. 384・1. 93-104 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maeda, M., Takeuchi, K., Kojima, M., Tanokura, M., Kimura, K., Amemiya, Y., Kihara, H., Takahashi, K.: "Kinetic studies of unfolding process of aspergillopepsinn II by pH-jump methods"Biochemical and Biophysical Research communications. 301・3. 745-750 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Katayama, H., Nagata, K., Ohira, T., Y.F., Tanokura, M., Nagasawa, H.: "The solution structure of molt-inhibiting hormone from the kuruma prawn Marsupenaeus japonicus"Journal of Biological Chemistry. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 田之倉優, 阿久津秀雄, 村松知成: "生化学キーノート(Hammes, B. D, Hooper, N. M. 著)"シュプリンガーフェアラーク東京. 464 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 田之倉優, 村松知成, 八木澤 仁: "分子生物学キーノート(Turner, P. C., McLennan, A. G., Bates, A. D., White, M. R. H. 著)"シュプリンガーフェアラーク東京. 398 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakai, N., Yao, N'I., Itou, H., Watanabe, N., Yumoto, F., Tanokura, M., Tanaka, I.: "The three-dimensional structure of septum site-determining protein MinD from Pyrococcus horikoshii OT3 in complex with Mg-ADP"Structure. 9・9. 817-826 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yumoto, F., Nara, M., Kagi, H., Iwasaki, W., Ojima, T., Nishita, K., Nagata, K., Tanokura, M.: "Coordination structures of Ca^<2+> and Mg^<2+>in Akazara scallop troponin C in solution : FTIR spectroscopy of side-chain COO^-Groups"European Journal of Biochemistry. 268. 6284-6290 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka, S., Ataka, M., Kubota, T., Soga, T., Homma, K., Lee, W.C., Tanokura, M.: "The effect of amphiphillic additives on the growth and morphology of Aspergillus niger acid proteinase A crystals"Journal of Crystal Growth. 234. 247-254 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kato, Y., Ito, M., Kawai, K., Nagata, K., Tanokura, M.: "Determinants of ligand specificity in groups I and IV WW domains as studied by surface plasmon resonance and model building"Journal of Biological Chemistry. 277(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taka,H.,Kaga,N.,Fujimura,T.Mineki,R.,Imaizumi,M.,Suzuki.Y.,Suzuki,R.,Tanokura,M.,Shindo,N.and Murayama,K: "Rapid determination of parvalbumin from Rana catesbeiana (pI4.78) by combination of ESI mass spectrometry, protein sequencing and amino acid analysis."Journal of Biochemistry. 64(4). 852-857 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki,R.,Nagata,K.,Kawakami,M.,Nemoto,N.,Furutani.M.,Adachi,K.,Maruyama.T.and Tanokura,M.: "Assignment of ^1H, ^<13>C and ^<15>N resonances of FKBP from Methanococcus thermolithotrophicus."Journal of Biochemistry. 17(2). 183-184 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murayama,K.,Shiodo,N.,Suzuki,R.,Kawakami,M.,Mineki,R.,Taka.H.,Kazuno,S.,Nagata,K.,Maruyama,T.and Tanokura,M.: "Characterization of native and recombinant peptidyl prolyl cis-trans isomerases derived from Methanococcus thermolithotrophicus based on cDNA sequence."Electrophoresis. 21. 1733-1739 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Onishi,H.,Konishi,K.,Fujiwara,K.,Hayakawa,K.,Tanokura,M.,Martinez,H.M.and Morales,M.F.: "On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide."Proceedings of National Academy of Sciences of U.S.A.. 97(21). 11203-11208 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagata,K.,Kudo,N.,Abe,K.,Arai,S.and Tanokura,M.: "Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L.japonica"Biochemistry. 39. 14753-14760 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kobori,T.,Sasaki,H.,Lee,W.C.,Zenno,S.,Saigo,K.,Murphy,M.E.P.and Tanokura,M.: "Structure and site- directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds. Alteration of pyridine nucleotide binding by a single amino acdi substitution."Journal of Biological Chemistry. 276(4). 2816-2823 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kobashi, N: "Kinetic and mutation analyses of aspartate kinase from Thermus thermophilus"J. Biosci. Bioeng.. 87. 739-745 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iwasaki, W.: "Crystallization and preliminary X-ray diffraction studies of a 40kDa calcium binding protein specifically expressed in plasmodia of Physarum polycephlum"J. Biochem. 126. 7-9 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tohyama, T.: "Gene structure and amino acid sequence of Latimeria Chalumnae (Coelacanth) myelin DM20: Phylogenetic relation of the fish"Neurochem. Res. 24. 867-873 (1999)

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      1999 Annual Research Report
  • [Publications] Kobori, T.: "Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli"Acta Crystallogr.. D55. 1901-1902 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe, S.: "Molecular cloning of the Lon protease gene from Thermus thermophilus HB8 and characterization of its gene product"Eur. J. Biochem. 266 (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kojima, M.: "pH-dependent unfolding of Aspergillus niger acid proteinase A studied by small-angle X-ray scattering."Biochemistry. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mitsuhashi, S.: "X-ray structure of beta-carbonic anhydrase from the red alga, porphyridium purpureum, reveals a novel catalytic site for CO(2) hydration"J. Biol. Chem.. 275. 5521-5526 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mitsuhashi, S.: "Crystallization and preliminary X-ray diffraction studies of a beta-carbonic anhydrase from the red alga porphyridium purpureum"Acta Crystallogr.. D56. 210-211 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mochizuki, M.: "Quantitative reevaluation of the redox active sites of crystalline bovine heart cytochrome oxidase"J. Biol. Chem.. 274. 33403-33411 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomizaki, T.: "Structure analysis of bovine heart cytochrome c oxidase at 2.8 A resolution"Acta Crystallogr. D55. 31-45 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naitow, H.: "A low-resolution structure of rice dwarf virus determined by ab initio phasing"Acta Crystallogr. D55. 77-84 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugimoto, H.: "Crystal structure of human D-dopachrome tautomerase, a homologue of macrophage migration inhibitory factor at 1.54【encircled a】 resolution"Biochemistry. 38. 3268-3279 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tominaga, T.: "High-Resolution Crystals of the HU Mutant K38N from Bacillus Stearothermophilus"J. Struct. Biol.. 125. 86-89 (1999)

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      1999 Annual Research Report
  • [Publications] Nakagawa, A.: "The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome"The EMBO J.. 18. 1459-1467 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] White, S.W.: "The high-resolution structure of DNA-binding protein HU from Bacillus Stearothermophilus"Acta Crystallogr. D55. 801-809 (1999)

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      1999 Annual Research Report
  • [Publications] Nakagawa, A.: "Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75A resolution"Biochem. Biophys. Acta. 1433. 253-260 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyamoto, A.: "Role of the N-terminal region of ribosomal protein S7 in its interaction with 16S rRNA which binds to the concavity formed by the β-ribbon arm and the α-helix"Eur. J. Biochem,. 266. 591-598 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saitoh, F.: "Arginine-55 in the β-Arm is Essential for the Activity of DNA-Binding Protein HU from Bacillus Stearothermophilus"Biosci. Biotech. Biochem.. 63. 2232-2235 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saito, J.: "Crystal structure of chitosanase from Bacillus circuland MH-K1 at 1.6A resolution and its substrate recognition mechanism"J. Biol. Chem.. 293. 57-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maeda, N: "Ribulose bisphosphate carboxylase/oxygenase from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 is composed solely of large subunits and forms a pentagonal structure."J. Mol. Biol.. 293. 57-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujihashi, M.: "Crystallization and preliminary X-ray diffraction studies of undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26"Acta Crystallogr.. 55. 1606-1607 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Komori, H.: "Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6A resolution"The EMBO J.. 18. 4597-4607 (1999)

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      1999 Annual Research Report
  • [Publications] Higuchi, Y.: "Removal of the bridging ligand atom at the Ni-Fe active Site of [NiFe] hydrogenase upon reduction with H2, as revealed by X-ray structure analysis at 1.4A resolution"Structure Fold Des.. 7. 548-556 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kita, A: "An archetypical extradiol-cleaving catecholic dioxygenase: the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Pseudomonas putida nt02"Structure Folds Des.. 7. 25-34 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugimoto, K.: "Purification and crystallization of a protocatechuate 4,5-dioxygenase LigAB from sphingomonas pausimobilis SYK-6"Protein and Peptide Lett.. 6. 55-58 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugimoto, K.: "Crystal structure of an aromatic-ring-opening dioxygenase LigAB, a protocatechuate 4,5-dioxygenase, in aerobic condition"Structure Fold Dex.. 7. 953-965 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugimoto, K.: "Purification and Crystallization of a Catechol 2,3-Dioxygenase PheB from Bacillus stearothermophilus BR219"Protein and Peptide Lett.. 6. 403-406 (1999)

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      1999 Annual Research Report
  • [Publications] Ohtsubo, Y.: "PcpA, which is involved in the degradation of pentachlorophenol in Sphingomonas chlorophenolica ATCC39723, is a novel type of ring-cleavage dioxygenase"FEBS Letters. 459. 395-398 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyoshima, C: "Structure determination of tubular crystals pf membrane proteins. I. Indexing of diffraction patterns"Ultramicroscopy. (in press). (2000)

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      1999 Annual Research Report
  • [Publications] Yonekura, K.: "Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes"Ultramicroscopy. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田之倉優: "タンパク質工学"丸善. 15 (1999)

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      1999 Annual Research Report
  • [Publications] 田之倉優: "魚介類筋肉タンパク質 その構造と機能"恒星社厚生閣. 8 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 豊島近: "蛋白質核酸酵素 構造生物学のフロンティア"共立出版(株). 9 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 千田俊哉: "蛋白質核酸酵素 構造生物学のフロンティア"共立出版(株). 7 (1999)

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  • [Publications] Tanabe,T.,Tanaka,N.,Nonaka,T.,Mitsui,Y.,Yoshimoto,T.et al.: "Roles of the Ser146,Tyr159 and Lys163 n the catalytic action of 7α-hydroxysteroid dehydrogenase." J.Biochem.(Tokyo). 124. 634-641 (1998)

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  • [Publications] Sugimoto,K.,Aoshima,H.,Senda,T.,Masai,E.,Fukuda,M.& Mitsui,Y: "Purification and crystallization of a protocatecuate 4,5-dioxygenase LigAB from Sphingomonas paucimobilis SYK-6." Protein and Peptide Letters. (in Press). (1999)

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  • [Publications] Sugimoto,K.,Senda,T.,Aoshima,H.Masai,E.,Fukuda,M.& Mitsui,Y.: "Crystal structure of an aromatic-ring-opening dioxygenase LigAB which is a protocatechuate 4,5-dioxygenase." Structure. (in Press). (1999)

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  • [Publications] H.Sakai & T.Tsukihara: "Structures of Membrane Proteins Determined at Atomic Resolution" J.Biochemistry. 124. 1051-1059 (1998)

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  • [Publications] T.Tsukihara: "Crystal Structural Studies of a Membrane Protein Complex,Cytochrome c Oxidase from Bovine Heart." Acta Crystallogr.A54. 895 (1998)

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  • [Publications] S.Yoshikawa et al.: "Redox-Coupled Crystal Structural Change in Bovine Herart Cytochrome c Oxidase." Science. 280. 1723-1729 (1998)

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  • [Publications] T.T.Tahirov et al.: "Crystal Structure of Methionine Aminopeptidase from Hyperthermophile,Pyrococcus furiosus." J.Mol.BIol.284. 101-124 (1998)

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  • [Publications] T.H.Tahirov et al.: "High-Resolution Crystal of Methionine Aminopeptidase from Pyrococcus furiosus Obtained by Water-Mediated Transformation" J.,Structural Biology. 121. 68-72 (1998)

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  • [Publications] I.Tanaka et al.,: "Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA." RNA. 4. 542-555 (1998)

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  • [Publications] J.Nishihira et al.,: "Molecular cloning of human D-dopachrome tautomerase cDNA:N-terminal proline is essential for enzyme activation" Biochem.Biophys.Res.Comm.243. 538-544 (1998)

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  • [Publications] K.Kitadokoro et al.: "Crystal Structure of Human Secretory Phospholipase A_2-IIA Complex with the Potent Indolizine Inhibitor 120-1032" J.Biochem.123. 619-623 (1998)

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  • [Publications] K.Kitano et al.: "A Novel Approach to Crystallizing Proteins with Temperature Induction Method:GrpE Protein from Thermus thermophilus" J.Cryst.Growth. 186. 456-460 (1998)

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  • [Publications] A.Osyczka et al.: "Interaction Site for Soluble Cytochromes on the Tetraheme Cytochrome Subunit Bound to the Bacterial Photosynthetic Reaction Center Mapped by Site-Directed Mutagenesis" Biochemistry. 37. 11732-11744 (1998)

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  • [Publications] H.Komori et al.: "Crystallization and Preliminary X-ray Diffraction Studies of a Replication Intiator Protein (RepE54) of the Mini-F Plasmid Complexes with Iteron DNA" J.Biochem.125. 24-26 (1999)

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  • [Publications] A.Kita et al.: "An archetypical extradiol-cleaving catecholic dioxygenase:the crystal structure of catechol 2,3-dioxygenase (metapyrocatechase)" Structure. 7. 25-34 (1999)

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  • [Publications] Zenno,S.: "Conversion of NfsA,the major Escherichia coli nitroreductase,to a flavin reductase having a specific activity similar to that of Vibrio harbeyi Frp,by a signale amino acid substitution." J.Bacteriol.180. 422-425 (1998)

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  • [Publications] Kono,H.: "Designing the hydrophobic core of Thermus flavus malate dehydrogenase based on side-chain packing." Protein Engineering. 11. 47-52 (1998)

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  • [Publications] Kudo,N.: "Crystallization and preliminary x-ray diffraction studies of a rice cysteine proteinase inhibitor,oryzacystatin-I." J.Biochem.123. 568-570 (1998)

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  • [Publications] Koike,H.: "1.8 Å crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium,Vibrio fischeri:overall structure,cofactor and substrate-analog binding,and comparison with related flavoproteins." J.Mol.Biol.280. 259-273 (1998)

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  • [Publications] Suzuki,Y.: "Evidence for existence of multiple conformations of kinesin and ncd motor domains in solution revealed by 31P NMR of the tightly bound ADP." Eur.J.Biochem.257. 466-471 (1998)

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  • [Publications] P.Zhang: "Structure of the calcium pump from sarcoplasmic reticulum at 8Å resolution" Nature. 392. 835-839 (1998)

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  • [Publications] H.Ogawa: "Structure of the Ca^<2+> pump of sarcoplasmic reticulum:A view along the lipid bilayer at 9-Å resolution" Biophys.J.75. 41-52 (1998)

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  • [Publications] K.Mayanagi: "Three-dimensional electron microscopy of the photosystem II core complex." J.Struct.Biol.(in press). (1998)

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  • [Publications] 三井幸雄、千田俊哉、野中孝昌: "「遺伝」 52巻、7号" X線で見る-生体高分子から生物マシーナリーへ-, 6 (1998)

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Published: 1998-04-01   Modified: 2018-03-28  

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