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Functional analysis of the new signal transduction enzyme PLD by the molecular genetic technique

Research Project

Project/Area Number 10212204
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (B)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionINSTITUTE OF APPLIED BIOCHEMISTRY (1999-2001)
Gifu University (1998)

Principal Investigator

NOZAWA Yoshinori  INSTITUTE OF APPLIED BIOCHEMISTRY, CHIEF, 部長 (10021362)

Co-Investigator(Kenkyū-buntansha) BANNO Yoshiko  GIFU UNIVERSITY SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50116852)
TANAKA Masashi  INSTITUTE OF APPLIED BIOCHEMISTRY, CHIEF, 部長 (60155166)
AKAO Yukihiro  INSTITUTE OF APPLIED BIOCHEMISTRY, CHIEF, 部長 (00222505)
中島 茂  岐阜大学, 医学部, 教授 (60188935)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥33,700,000 (Direct Cost: ¥33,700,000)
Fiscal Year 2001: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2000: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1999: ¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1998: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsPHOSPHOLIPASE D / SURVAIVAL / SPHINGOSINE 1-PHOSPHATE / PHOSPHATIDYLINOSITOL 3-KINASE / AKT / 癌化 / H_2O_2 / ERK / P38MAPK / 遺伝子導入 / 細胞機能解析 / 情報変換酵素 / 情報伝達 / 分泌応答 / 増殖 / 分化 / アポトーシス / 老化
Research Abstract

In order to clarify the role of the phospholipase D (PLD) isozyme PLD1 and PLD2 in cell functions, PLD1 and PLD2 cDNA were transfected into various cells, and the influences on the signal transduction and cell functions were examined. At the early stages of apotosis induced by H_2O_2 or low oxygen in PC12 cell, PLD activity was increased transiently. The activation of PLD by H_2O_2 was attributed to PLD2, and was activated through the MAP kinase family (ERK and p38MAP kinase). Moreover, the apotosis induced by low oxygen was suppressed by transfection of PLD2 into PC12 cells, suggesting that PLD acts to prevent apotosis. It has been known that sphingosine 1-phosphate (S1P) is mitogen and participates in a cell survival. We examined a role of PLD in the survival signaling PI3 kinase and Akt using the CHO cell overexpressed with the S1P receptor EDG3. S1P stimulation of EDG3-CHO cells, but not vector-transfected cells, induced activation of PLD, PI3-kinase, and Akt. The Akt phosphorylati … More on was prevented by the PI3-kinase inhibitors wortmannin and LY294002, indicating that the Akt activation was dependent on PI3-kinase. S1P-induced activation of PI3-kinase and Akt was abrogated by 1-butanol, which inhibited S1P-induced accumulation of phosphatidic acid (PA) by PLD, whereas it was not inhibited by 2-butanol without such an action. Coexpression of the wild-type PLD2 with myc-Akt resulted in increased Akt activation in response to S1P. In contrast, co-expression of a catalytically inactive mutant of PLD2 eliminated the S1P-induced Akt activation. The treatment of EDG3-CHO cells with exogenous Streptomyces chromofuscus PLD, which caused an accumulation of PA, resulted in increases in PI 3-kinase activity and the phosphorylation of Akt, the latter of which was completely abolished by LY294002. Furthermore, SIP-induced membrane ruffling, which was dependent on PI 3-kinase and Rac, was inhibited by 1-butanol, but not 2-butanol. These results demonstrate that PLD participates in the activation of PI 3-kinase and Akt in stimulation of EDG3 receptor. Moreover, the remarkable high PLD2 activity was found in a human renal cancer. Immuno-stainining with the antibody of PLD2 revealed strong positive stain in the nuclei of a renal cancer compared with the normal tissue, suggesting that PLD2 may be associated with the participation in tumorigenesis. Less

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (106 results)

All Other

All Publications (106 results)

  • [Publications] Banno, Y. et al.: "Involvement of PLD in S-1-P-induced activation of phosphatidylinositol 3-kinase and Akt in Chinese hamster ovary cells overexpressing EDG3"J. Biol. Chem.. 276. 35622-35628 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Banno, Y. et al.: "Involvement of ERK and p38 MAP kinase in oxidative stress-induced-phospholipase D activation in PC12 cells"NeuroReport. 12. 2271-2275 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, M. et al.: "p53 regulates ceramide formation by neutral sphingomyelinase through reactive oxygen species in human glioma cells"Oncogene. 20. 1368-1378 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, Y. et al.: "TNF-α induced sphingosine 1-phosphate inhibits apoptosis through a phosphatidylinositol 3-kinase/Akt pathway in human hepatocytes"J. Immnunol.. 167. 173-180 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murate, T. et al.: "Cell type-specific localization of sphingosine kinase 1a in human tissues"J. Histochem. Cytochem.. 49. 845-855 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, Y. et al.: "Sphingosine kinase regulates hepatoma cell differentiation : roles of hepatocyte nuclear factor and retinoid receptor"Biochem. Biophys. Res. Commun.. 286. 673-677 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 野澤義則 他: "Navigator, Vascular Biologyナビゲーター"メディカルレビュー社,丸山征郎,安藤譲二,佐藤靖史編. 2 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Czarny, M.: "Phospholipase D2 : functional interaction with caveolin in Low-density membrane microdomains"FEBS Lett.. 467. 326-332 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dojima, T.: "Preferential down-regulation of phospholipase C-b in Ewing's Sarcoma cells transfected with antisense EWS-Fli-1"British J. Cancer. 82. 16-19 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamakawa, H: "Activation of caspase-9 and -3 during H2O2-induced apoptosis of PC12 cells independent of ceramide formation"Neurol. Res.. 22. 556-564 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, M: "Influence of Bax or Bcl-2 overexpression on the ceramidedependent apoptic pathway in glioma cells"Oncogene. 19. 3508-3520 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamakawa, H: "Increased phospholipase D2 activity during hypoxia-induced death of PC12 cells : Its possible anti-apoptotic role"Neuro Report. 11. 3647-3650 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koike, T.: "Activation of MMP-2 by Clostridium difficile Toxin B in bovine smooth muscle cells"Biochem. Biophys. Res. Commun.. 277. 43-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sawada, M: "Ordering of ceramide formation, caspase activation, and Bax/Bcl-2 expression during etoposide-induced apoptosis in C6 glioma cells"Cell Death Differ.. 7. 761-772 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zha, Y.: "Increased activity and intranuclear expression of phospholipase D2 in human renal cancer"Biochem. Biophys. Res. Commun.. 278. 140-143 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oishi, K.: "PKN regulates phospholipase Dl through direct interaction"J. Biol. Chem.. 276. 18096-18101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang, S.: "Enzymatic characterization of phospholipase D of : protozoan Tetrahymena cells"J. Eur. Mycrobiol.. 48. 194-201 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, Y.: "Possible involvement of reactive oxygen species in D-galactosamine-induced sencitization against tumor necrosis factor-α-induced hepatocyte apoptosis"J. Cell. Physiol.. 187. 374-385 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, Y.: "TNF-α induced sphingosine 1-phosphate inhibits apoptosis through a phosphatidylinositol 3-kinase/Akt pathway in human hepatocytes"J. Immunol.. 167. 173-180 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Banno, Y.: "Involvement of ERK and p38 MAP kinase in oxidative stress-induced-phospholipase D activation in PC12 cells"Neuro Report. 12. 2271-2275 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murate, T.: "Cell type-specific localization of sphingosine kinase 1a in human tissues"J. Histochem. Cytochem.. 49. 845-855 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa ,Y: "Sphingosine kinase regulates hepatoma cell differentiation roles of Hepatocyte nuclear factor and retinoid receptor"Biochem. Biophys. Res. Commun.. 286. 673-677 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Banno, Y.: "Involvement of phospholipase D in sphingosine 1-phosphate-induced activation of phosphatidylinositol 3- kinase and Akt in Chinese hamster ovary cells overexpressing EDG3"J. Biol. Chem.. 276. 35622-35628 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, Y: "Caspase activation during hepatocyte apoptosis induced by tumor necrosis factor-a and D-galactosamine"Liver. 21. 309-319 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamakawa, H.: "Crucial role of calpain in hypoxic PC12 cell death; calpain, but not caspases, mediates degradation of cytoskeletal proteins and protein kinase C-α and -δ"Nerol. Res.. 23. 522-529 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tan, Z.: "Involvement of mitogen-activated protein kinase family in the tetracaine-induced apoptotic PC12 cell death"Anesthesiology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashimoto, K.: "Co-overexpression of DEAD box protein rck/p54 and c-myc in human colorectal adenomas and the relevance of their expression in cultured cell lines"Carcinogenesis. 22. 1965-1970 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, T.: "Vaticanol C, a novel resveratrol tetramer, inhibits cell growth through induction of apoptosis in colon cancer cell lines"Biol. Pharm. Bull.. 25. 147-148 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maruyama, W.: "Transfection-enforced Bcl-2 overexpression and an anti-Parkinson drug, rasagiline, prevent nuclear accumulation of glyceraldehydes-3-phosphate dehydrogenase induced by an endogenous dopaminergic neurotoxin, N-methyl (R) salsolinol"J. Neurochem. 78. 727-735 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakagawa, Y.: "Arsenic trioxide-induced apoptosis through oxidative stress in cells of colon cancer cell lines"Life Sciences. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akao, Y.: "An anti-Parkinson's disease drug, N-propargyl-1R-aminoindan (rasagiline), enhances expression of anti-apoptotic Bcl-2 in dopaminergic SH-SY5Y cells"Neurosci. Lett.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akao, Y.: "Apoptosis induced by N-methyl (R)-salsolinol, an endogenous neurotoxin, is mediated by mitochondrial permeability transition and suppressed by Bcl-2 and rasagiline, N-propargyl-1(R) - aminoindan. J"Neurochem. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Naoi, M.: "Mitochondria determine the survival and death in apoptosis by an endogenous neurotoxin, N-methyl (R) salsolinol, and neuroprotection by propargylamines"J. Neural Transm. Suppl, in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Naoi, M.: "Dopamine-derived endogenous N-methyl (R) salsolinol"Neurotoxicol. Teratol.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimokata, H.: "Distribution of geriatric disease-related genotypes in the National Institute for Longevity Sciences, Longitudinal Study of Aging (NILS- LSA)"J. Epidemiol.. 10. S46-S55 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin, W.S.: "A novel homoplasmic mutation in mtDNA with a single evolutionary origin as a risk factor for cardiomyopathy"Am J Hum Genet.. 67. 1617-1620 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, M.: "Mitochondrial genotype associated with longevity and its inhibitory effect on mutagenesis"Mech Ageing Dev.. 116. 65-76 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, M.: "Common, rare, and individual variations of mitochondrial DNA associated with diseases and longivity"J Clin Biochem Nutr.. 28, in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsunaga, H.: "Antiatherogenic mitochondrial genotype in patients with type 2 diabetes"Diabetes Care. 24. 500-503 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mizutani, J.: "Unique mutations in mitochondrial DNA of senescence-accelerated mouse (SAM) strains"J Hered. 92. 352-355 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sahashi, K.: "Functional characterisation of mitochondrial tRNA(Tyr) mutation (5877-->GA) associated with familial chronic progressive external ophthalmoplegia"J Med Genet.. 38. 703-705 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, M.: "Mitochondrial DNA Polymorphisms and Aging"Environ Mutagen Res.. 23. 197-205 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terasaki, F.: "A case of cardiomyopathy showing progression from the hypertrophic to the dilated form : association of Mt8348A->G mutation in the mitochondrial tRNA (Lys) gene with severe ultrastructural alterations of mitochondria in cardiomyocytes"Jpn Circ J.. 65. 691-694 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Umetsu, K.: "Multiplex amplified product-length polymorphism analysis for rapid detection of human mitochondrial DNA variations"Electrophoresis. 22. 3533-3538 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada, Y.: "Association of a G994->T (Val279->Phe) polymorphism of the plasma platelet-activating factor acetylhydrolase gene with myocardial damage in Japanese patients with nonfamilial hypertrophic cardiomyopathy"J Hum Genet.. 46. 436-441 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada, Y.: "Association of the C-509->T polymorphism, alone or in combination with the T869->C polymorphism, of the transforming growth factor-beta1 gene with bone mineral density and genetic susceptibility to osteoporosis in Japanese women"J Mol Med.. 79. 149-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fuku, N.: "Mitochondrial ATPase subunit 6 and cytochrome b gene polymorphisms in young obese adults"Biochem Biophys Res Commun.. 290. 1199-1205 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, M.: "Gene therapy of mitochondrial disease by delivering restriction endonuclease SmaI into mitochondria"J Biomed Sci.. in press. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, M.: "Golden mean to longevity : Rareness of mitochondrial cytochrome b variants in centenarians but not in patients with Parkinson's disease"J Neurosci Res.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto, T.: "Selective nitration of mitochondrial complex I by peroxynitrite : involvement in mitochondria dysfunction and cell death of dopaminergic SH-SY5Y cells"J Neural Transm.. 109. 1-13 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Banno, Y. et al.: "Involvement of PLD in S-1-P-induced activation of phosphatidylinositol 3-kinase and Akt in Chinese hamster ovary cells overexpressing EDG3"J.Biol.Chem.. 276. 35622-35628 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Banno, Y. et al.: "Involvement of ERK and p38 MAP kinase in oxidative stress-induced-phospholipase D activation in PC12 cells"NeuroReport. 12. 2271-2275 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sawada, M. et al.: "p53 regulates ceramide formation by neutral sphingomyelinase through reactive oxygen species in human glioma cells"Oncogene. 20. 1368-1378 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Osawa, Y. et al.: "TNF-α induced sphingosine 1-phosphate inhibits apoptosis through a phosphatidylinositol 3-kinase/Akt pathway in human hepatocytes"J.Immunol.. 167. 173-180 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Murate, T. et al.: "Cell type-specific localization of sphingosine kinase la in human tissues"J.Histochem.Cytochem.. 49. 845-855 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Osawa, Y. et al.: "Sphingosine kinase regulates hepatoma cell differentiation : roles of hepatocyte nuclear factor and retinoid receptor"Biochem.Biophys.Res.Commun.. 286. 673-677 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 野澤義則 他: "Navigator, Vascular Biologyナビゲーター"メディカルレビュー社, 丸山征郎, 安藤譲二, 佐藤靖史編. 2 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Czarny,M.,Fiucci,G.,Lavie,Y.,Banno,Y.,Nozawa,Y.,Liscovitch,M.: "Phospholipase D2 : functional interaction with caveolin in low-density membrane microdomains"FEBS Lett.. 467. 326-332 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Dojima,T.,Ohno,T.,Banno,Y.,Nozawa,Y.,Wen-yi,Y.,Shimizu,K.: "Preferential down-regulation of phospholipase C-b in Ewing's sarcoma cells transfected with antisense EWS-Fli-1"Brit.J.Cancer. 82. 16-19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamakawa,H.,Banno,Y.,Nakashima,S.,Sawada,M.,Yamada,I.,Yoshimura,S.,Nishimura,Y.,Nozawa,Y.,Sakai,N.: "Increased phospholipase D2 activity during hypoxia-induced death of PC12 cells : Its possible anti-apoptotic role"NeuroReport. 11. 3647-3650 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koike,T.,Kuzuya,M.,Asai,T.,Kanda,S.,Cheng,X.W.,Banno,Y.,Nozawa,Y.,Watanabe,K.,Iguchi,A.: "Activation of MMP-2 by Clostridium difficile Toxin B in bovine smooth muscle cells"Biochem.Biophys.Res.Commun.. 277. 43-46 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Han,X.Y.,Ito,Y.,Nozawa,Y.,Jiang,Y.,Matsunami,K.: "Effect of APGW-amide on [Ca^<2+>] in rat pheochromocytoma PC12 cells"Jpn.J.Pharmacol.. 84. 355-359 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zha,Y.,Ehara,H.,Akao,Y.,Shamoto,M.,Nakagawa,Y.,Banno,Y.,Deguchi,T.,Ohishi,N.,Yagi,K.,Nozawa,Y.: "Increased activity and intranuclear expression of phospholipase D2 in human renal cancer"Biochem.Biophys.Res.Commun.. 278. 140-143 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 野澤義則: "細胞研究モデルとしての血小板,血小板-大きな活力を秘めた小さな血球,池田康夫,山崎博男 監修"恒陽社(担当2頁). 87 (2000)

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      2000 Annual Research Report
  • [Publications] Ohguchi, K. et al.: "Phospholipase D development during differentiation of human promyelocytic leukemic HL60 cells"Biochim. Biophys. Acta.. 1439. 215-227 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Banno, Y. et al.: "Differential phospholipase D activation by bradykinin and sphingosine 1-phosphate in NIH 3T3 fibroblasts overexpressing gelsolin"J. Biol. Chem.. 274. 27385-27391 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakamura, Y. et al.: "Stimulatory effect of cytocharasin D on antigen-induced phospholipase D activation in a murine mast cell model(RBL-2H3)"Allergol. Intern.. 84. 239-245 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tan, Z. et al.: "Effects of local anesthetics on phospholipase D activity in differentiated human promyelocytic leukemic HL60 cells"Biochim. Pharmacol.. 58. 1881-1889 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kumada, H. et al.: "Hydrogen peroxide-induced phospholipase D activation in human vascular smooth muscle cells"Biomed. Res.. 20. 87-98 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Czarny, M. et al.: "Phospholipase D2 : functional interaction with caveolin in low-density membrane microdomains"FEBS Lett.. 467. 326-332 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakashima,S.et al.: "Increased mRNA expression of phospholipase D(PLD) isozymes during granulocytic differentiation of HL60 cells" Biochim.Biophys.Acta. 1389. 173-177 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimura,S.et al.: "Ceramide formation leads to caspase-3 Activation during hypoxic PC12 cell death : Inhibitory effects of Bcl-2 on ceramide formation and caspase-3 activation" J.Biol.Chem.273. 6921-6927 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,H.et al.: "Molecular cloning of a cDNA encodig a serine protease homologous to complement C1s precursor from rat C6 glial cells and its expression during glial differentiation" Gene. 209. 87-94 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,M.et al.: "Involvement of tyrosine phosphorylation in inhibition of fMLP-induced PLD activation by N-acetyl-L-cysteine in differentiated HL-60 cells" J.Leuk.Biol.63. 781-789 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanoh,H.et al.: "Molecular cloning of a gene encoding phospholipase D from the pathogenic and dimorphic fungus,Candida albicans" Biochim.Biophys.Acta. 1398. 359-364 (1998)

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      1998 Annual Research Report
  • [Publications] Leidich,S.D.et al.: "Cloning,and disruption of the Candida albicans phospholipase B gene,CaPLB1" J.Biol.Chem.273. 26078-26086 (1998)

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      1998 Annual Research Report
  • [Publications] Wang,S.et al.: "Molecular cloning and cell cycle-dependent expression of a novel NIMA-related protein kinase (TpNrK) in Tetrahymena cells" Biochem.J.334. 197-203 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizutani,T.et al.: "Changes in the expression of protein kinase C(PKC),phospholipases C(PLC) and D(PLD) isofoms in spleen,brain and kidney of the aged rat : RT-PCR and Western blot analysis" Mech.Ageing Develop.105. 151-172 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayakawa,K.et al.: "Decreased phospholipase D activities in ceramide-induced apoptosis of RBL-2H3 mast cells." Biomed.Res.19. 159-170 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okamoto,H.et al.: "Studies on blood compatibility of terpolymers composed of methylmethacrylate methoxypolyethyleneglycol methacrylate and dimethylsiloxane methacrylate" J.Biomater.Sci.Polymer Edn.9. 943-959 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ito,Y.et al.: "Possible involvement of mitogen-activated protein kinase in phospholipase D activation induced by H_2O_2,but not by carbachol,in rat pheochromocytoma PC12 cells" J.Neurochem.71. 2278-2285 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kasai,T.et al.: "Increased activity of oleate-dependent type phospholipase D during actinomycin D-induced apoptosis in Jurkat T cells" J.Immunol.161. 6469-6474 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwasaki-Bessho,Y.et al.: "Decreased phospholipase D(PLD) activity in ceramide-induced apoptosis of human keratinocyte cell line HaCaT" J.Invest.Dermatol.110. 376-382 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ojio,K.et al.: "Role of Rho family GTP-binding proteins in IgE receptor-mediated phospholipase D activation in mast cells" Biomed.Res.19. 53-63 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Banno,Y.et al.: "Stimulation by G protein βγ subunits of phospholipase Cβ isoforms in human platelets" Thromb.Haemost.79. 1008-1013 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Banno,Y.et al.: "Evidence for the presence of multiple forms of sphingosine kinase in human platelets" Biochem.J.335. 301-304 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nomura,T.et al.: "Identification of GTP cyclohydrolase I activity in the ciliated protozoa,Tetrahymena pyriformis." Comp.Biochem.Physiol.120B. 753-760 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagata,K.et al.: "Expression of GTP-binding proteins and protein kinase C isozymes in platelet-like particles derived from megakaryoblastic leukemia cells (MEG-O1)" Platelets. 9. 291-296 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 坂野喜子 他: "細胞膜脂質シグナリングの分子機構" 日本生理学雑誌. 60. 43-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 加納宏行 他: "Candida albicansのPLD遺伝子クローニング" 真菌誌. 39. 79-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大口健司 他: "好中球機能とホスホリパーゼD" 炎症と免疫. 16. 37-43 (1998)

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      1998 Annual Research Report
  • [Publications] 大口健司 他: "シグナル伝達におけるPKCとPLD" 生体の科学. 49. 248-252 (1998)

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      1998 Annual Research Report
  • [Publications] 坂野喜子 他: "膜リン脂質代謝" 蛋白質核酸酵素. 43. 1800-1805 (1998)

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      1998 Annual Research Report
  • [Publications] Takanaka,K.et al.: "Analysis for phospholipase C gene in patients with subarachnoid hemarrhage due to ruptured intracranial saccular aneurysm" Neurol.Res.(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugiyama,Y.et al.: "Molecular cloning of a second phospholipase B gene,caPLB2 from Candida albicans." J.Med.Mycol.(印刷中).

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      1998 Annual Research Report
  • [Publications] Ohguchi,K.et al.: "Phospholipase D development during differentiation of HL60 cells" Biochim.Biophys.Acta. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakashima,S.et al.: "Molecular cloning and expression of a stress-responsive MAPK-related kinase from Tetrahymena cells" J.Biol.Chem.(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Saji,S.et al.: "Overexpression of MDM2 in MCF-7 promotes both growth advantage and p53 accumulation in response to estradiol" Jap.J.Cancer Res.(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Takenaka,K.et al.: "Polymorphism of the endoglin gene in patients with intracranial saccular sneurysm" J.Neurosurg.(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakashima,S.et al.: "Possible role of phospholipase D(PLD) in cellular differentiation and apoptosis" Chem.Phys.Lipids. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimizu,H.et al.: "Activation of p38 mitogen-activated protein kinase and caspases in UVB-induced apoptosis of human keratinocyte HaCaT cells" J.Invest.Dermatol.(印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakashima,S.et al.: "Stress responsive gene expression in Tetrahymena" Acta Biologica Hungarica. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 中島 茂 他: "ホスホリパーゼDのシグナルトランスダクションにおける役割" 蛋白質核酸酵素. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 坂野喜子 他: "ホスホリパーゼC(PLC)" 現代医療. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 野澤義則: "細胞内情報伝達に関与する膜脂質シグナリングシステム概説" 蛋白質核酸酵素. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 坂野喜子 他: "ホスホリパーゼC" 蛋白質核酸酵素. (印刷中).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2018-03-28  

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