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Mechanisms of recombination mediated by DNA end-joining

Research Project

Project/Area Number 10216201
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Genetics (2002-2003)
Kitasato Institute (1999-2001)
The University of Tokyo (1998)

Principal Investigator

OGATA Yasuyuki (2002-2003)  National Institute of Genetics, Radiation Isotope Center, Research follow, 放射線・アイソトープセンター, 助手 (90344449)

池田 日出男 (1998-2001)  社団法人北里研究所, 基礎研究所, 部長 (10012775)

Co-Investigator(Kenkyū-buntansha) NIKI Hironori  National Institute of Genetics, Radiation Isotope Center, Associate Professor, 放射線・アイソトープセンター, 助教授 (70208122)
IKEDA Hideo  Medinet Inc., Institute of Molecular Genetics, Director, 分子遺伝学研究所, 所長(研究職) (10012775)
小方 康至  国立遺伝学研究所, 放射線・アイソトープセンター, 助手
舘林 和夫  東京大学, 医科学研究所, 助手 (50272498)
林 正彦  社団法人北里研究所, 基礎研究所, 室長 (20164965)
加藤 潤一  東京大学, 医科学研究所, 助手 (10194820)
Project Period (FY) 1998 – 2002
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥88,300,000 (Direct Cost: ¥88,300,000)
Fiscal Year 2002: ¥18,500,000 (Direct Cost: ¥18,500,000)
Fiscal Year 2001: ¥19,000,000 (Direct Cost: ¥19,000,000)
Fiscal Year 2000: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 1999: ¥17,600,000 (Direct Cost: ¥17,600,000)
Fiscal Year 1998: ¥17,000,000 (Direct Cost: ¥17,000,000)
KeywordsChromosomal aberration / DNA double-strand break / DNA end-joining / Illegitimate recombination / DNA gyrase / RecQ helicase / RecROF proteins / Sgsl helicase / 非相同的組換え / DNA二重鎖切断 / 増殖静止期 / 飢餓 / DNAアルキル化 / adaptive mutation / adaptive amplification / RecEエキソヌクレアーゼ / H-NSタンパク質 / Tag DNAグリコシラーゼ / DNAトポイソメラーゼII / WRNヘリケース / DnaBヘリケース / DNA損傷 / DNAリガーゼ / 遺伝的組換え / サイレンシング因子 / 修復 / 組換え / Ku蛋白質 / DNAヘリケース / 老化 / SGSI遺伝子 / DNAヘリカーゼ / BLM遺伝子 / WRN遺伝子 / DNA損傷のチェックポイント
Research Abstract

Mechanisms of illegitimate recombination have been analyzed by newly developed assay systems for illegitimate, recombination in Escherichia coli, yeast and mouse. Based on the analyses of E. coli DNA gyrase mutants that confer a hyper-recombination phenotype, we showed that several (α-helix structures of DNA gyrase plays an important role in illegitimate recombination. It is also shown that E. coli DNA binding protein HU has an activity that suppress DNA gyrase-mediated illegitimate recombination, thus playing a regulatory role in the recombination. We also indicated that DnaB helicase, RecJ exonuclease, RecE exonuclease, RecT, RecO, RecR, RecF proteins, and DNA ligase are involved in DNA damage-induced illegitimate recombination in E. coli. Furthermore, many recombination and repair enzymes, RecQ helicase, UvrA, UvrB, MutM, Tag DNA glycosylase, AlkA DNA glycosylase, exonuclease I (SbcB), and H-NS play important roles for suppression of DNA damage-induced illegitimate recombination. We further indicated that when bacteria are grown under a starving condition, alkylated DNA lesions are spontaneously accumulated, thus leading to the promotion of DNA double-strand breaks and illegitimate recombination. In Saccharomyces cerevisiae, we showed that a DNA topoisomerase II inhibitor VP16 induces illegitimate recombination, suggesting that DNA topoisomerase II promotes illegitimate recombination through DNA double-strand break and end-joining. It is, on the other hand, shown that Sgs1 protein suppresses spontaneous illegitimate recombination. Human BLM and WRN proteins also suppressed illegitimate recombination in budding yeast. In addition, we showed that budding yeast Scc1 and fission yeast Dhp1 proteins are involved in DNA repair and chromosome segregation and that budding yeast Whip and fission yeast Mog1 and Hsk1 kinase interact with Sgs1 and Scc1, respectively. Based on these results, we proposed several models for illegitimate recombination in E. coli and yeast.

Report

(6 results)
  • 2003 Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (101 results)

All Other

All Publications (101 results)

  • [Publications] Heo, S.-J., Tatebayashi, K., Kato, J., Ikeda, H.: "Budding yeast RHC21 gene, a homologue of fission yeast rad21^+ gene, is essential for chromosome segregation."Mol.Gen.Genet.. 257. 149-156 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatebayashi, K., Kato, J., Ikeda, H.: "Isolation of a Schizosaccharornyces poinbe rad21ts muatni which is abenant in chromosome segregation, microtuhule function, DNA repair and DNA replication : possible involvement of Rad21 in ubiauitin-rnediated proteolysis."Genetics. 148. 49-57 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsukamoto, Y., Ikeda, H.: "Double-strand break repair mediated by DNA end-joining."Genes Cells. 3. 135-144 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamagata, K., Kato, J., Shimamoto, A., Goto, M, Furuichi, Y., Ikeda, H.: "Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgsl mutant : Implication for genomnic instability in human diseases."Proc.Natl.Acad.Sci.USA. 95. 8733-8738 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shanado, Y., Kato, J., Ikeda, H.: "Escherichia coli HU protein suppresses DNA-gyrase-mediated illegitimate recombination and SOS induction."Genes Cells. 3. 511-520 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, Y., Shimamoto, A., Shohuike, T., Sugimoto, M., Ikeda, N., Kuroda,.S., Furiichi.Y.: "Cloning and characterization of human Sep1 (hSEP1) gene and cytoplasmic localization of its product."DNA Res.. 5. 241-246 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 塚本恭正, 池田日出男: "DNAのエンドジョイニングによる修復と組換え-サイレンシング因子の関与"細胞工学. 17. 264-265 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onda, M., Hanada, K., Kawachi, H., Ikeda, H.: "H.Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress."Genetics. 151. 439-446 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa, Y, Yokochi, T., Ogata, Y., Shobuike.Y., Kato, J., Ikeda I-L: "Mechanism of DNA gyrase-mediated illegitimate recombination : Characterization or Ewherichia coli gyrA mutations that confer hyperrecombination phenotype."J.Mol.Biol.. 289. 447-458 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Heo, S.-J., Tatebayashi, K., Ikeda, H.: "The budding yeast cohesin gene SCC1/MGD1/RHC21 genetically interacts with PKA, CDK and APC."Curr.Genet.. 36. 329-338 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamashita., T., Hanada, K., Iwasaki, M., Yamaguchi, H., Ikeda, H.: "Illegitimate recombination induced by overproduction of DnaB helicase in Eschierichia coli."J.Bacteriol.. 181. 4549-4553 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nohmi, T., Suzuki, M., Ueda, O., Suzuki, H., Katoh, M., Ikeda.H., Sofuni, T.et al.: "Spi(-) selection : An efficient method to detect gamma-ray-induced deletions in transgenic mice."Environ.Mol.Mutagen. 34. 9-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Heo, S.-J., Tatebayashi, K., Ohsugi, I., Shimamoto, A., Furuichi, Y., Ikeda, H.: "Bloom's syndrome gene suppresses premature aging caused by Sgsl deficiency in yeast."Genes Cells. 4. 619-625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 花田克浩, 池田日出男: "DNA2本鎖切断とエンドジョイニング複合体"蛋白質核酸酵素. 44. 1838-1844 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 池田日出男, 小方康至: "DNA非相同組換え-DNAエンドジョイニングによる修復反応"細胞工学. 18. 984-988 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小方康至, 池田日出男: "環境のストレスによって誘導される非相同的組換え-DNAの切断と再結合の分子機構"環境変異原研究. 21. 11-21 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, H., Hanada, K., Asami, Y., Kato, J., Ikeda, H.: "Control of genetic stability in Evchericliia coli ; the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease."Genes Cells. 5. 101-109 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hanada, K, Iwasaki, M., Ihashi, S., Ikeda, H.: "UvrA and UvrB suppresses illegitimate recombination : Synergistic action with RecQ helicase."Proc.Natl.Acad.Sci.USA. 97. 5989-5994 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 池田日出男: "DNAのエンドジョイニングの分子機構:2種類の非相同的組換え"環境変異原研究. 22. 107-113 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kang, S.-W., Ikeda, H.: "WRN homologues in non-human systems. In Werner syndrome : Implications for aging and cancer."GANN MONOGRAPH ON CANCER RESEARCH. 49. 137-145 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onda, M., Ikeda, H.: "Role of DNA ligase in the illegitimate recombination that generates λ bio transducing phages."Genetics. 158. 29-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shanado, Y., Hanada, K., Ikeda, H.: "Suppression of gamma-ray-induced illegitimate recombination in Ewherichia coli by the DNA binding protein H-NS."Mol.Gen.Genet.. 265. 242-248 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast."Nucleic Acid Res.. 29. 1326-1333 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatebayashi, K., Tani, T., Ikeda, H.: "Fission yeast Toilp, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism."Genetics. 157. 1513-1522 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeda, T., Ogino, K., Tatebaynshi, K., Ikeda, H., Arai, K., Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast"Mol.Biol.Cell.. 12. 1257-1274 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hanada, K., Yamashita, T., Shobuike, Y., Ikeda, H.: "Role of DnaB helicase in UV-induced illegitimate recombination in Escherichia coli."J.Bacteriol.. 183. 4969-4969 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawabe, Y., Branzei, D., Ikeda, H., Shimamoto, A., Furuichi, Y., Seki, M, Enomoto, T., et al.: "A novel protein interacts with the Werner's syndrome gene product physically and functionally."J.Biol.Chem.. 276. 20364-20369 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 花田克浩, 池田日出男: "DNAの切断再結合による非相同組換え"蛋白質 核酸 酵素. 46. 1063-1072 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asami, Y., Jia, D.-W., Tatebayashi, K., Yamagata, K., Tanokura, M., Ikeda, H.: "Effect of DNA topoisomerase II inhibitor VP-16 on illegitimate recombination in yeast chromosome."Gene. 291. 251-257 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiraishi, K., Hanada, K., Iwakura, Y., Ikeda, H.: "Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli."J.Bacteriol.. 181. 4715-4721 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sabourin, M, Nitiss, J.L., Nitiss, K.C., Tatebayashi, K., Ikeda, H., Osheroff, N.: "Yeast recombination pathways triggered by topoisomerase IL-mediated DNA breaks."Nucleic Acids Res.. 31. 4373-4384 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Janscak, P., Garcia.P.I., Hamburger, F., Makuta, Y., Shiraishi, K., Imai, Y., Ikeda H, Bickle, T.A.: "Characterization and mutational analysis of the RecQ core of the Bloom Symdrome protein."J.Mol.Biol.. 330. 29-42 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小方慶至, 仁木宏典: "バクテリアにおける細胞周期に応じたタンパク質と核酸の動的な局在変化"生化学. 75. 137-143 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, H., Shiraishi, K., Ogata, Y.: "IIlegitimate recombination mediated by double-strand break and end-joining in Escherichia coli."Adv.Biophys.. 38. 1-18 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Heo, S.-J., Tatabayashi, K., Kato, J., Ikeda, H.: "Budding yeast RHC21 gene, a homologue of fission yeast rad21^+ gene, is essential for chromosome segregation."Mol. Gen. Genet.. 257. 49-156 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatebayashi, K., Kato, J., Ikeda, H.: "Isolation of a Schizosaccharomyces pomb rad21ts muatnt which is aberrant in chromosome segregation, microtubule function, DNA repair and DNA replication : possible involvement of Rad21 in ubiquitin-mediated proteolysis."Genetics. 148. 49-57 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsukamoto, Y., Ikeda, H.: "Double-strand break repair mediated by DNA end-joining."Genes Cells. 3. 135-144 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamagata, K., Kato, J., Shimamoto, A., Goto, M., Furuichi, Y., Ikeda, H.: "Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant : Implication for genomic instability in human diseases."Proc. Natl. Acad. Sci. USA. 95. 8733-8738 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shanado, Y., Kato, J., Ikeda, H.: "Escherichia coli HU protein suppresses DNA-gyrase-mediated illegitimate recombination and SOS induction."Genes Cells. 3. 511-520 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, Y., Shimamoto, A., Shobuike, T., Sugimoto, M., Ikeda, H., Kuroda, S., Furiichi, Y.: "Cloning and characterization of human Sep1 (hSEP1) gene and cytoplasmic localization of its product."DNA Res.. 5. 241-246 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onda, M., Hanada, K., Kawachi, H., Ikeda, H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress."Genetics. 151. 439-446 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa, Y., Yokochi, T., Ogata, Y., Shobuike, Y., Kato, J., Ikeda, H.: "DNA gyrase-mediated illegitimate recombination : Characterization of Escherichia coli gyrA mutations that confer hyperrecombination phenotype."J. Mol. Biol.. 289. 447-458 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Heo, S.-J., Tatebayashi, K., Ikeda, H.: "The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC."Curr. Genet.. 36. 329-338 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamashita, T., Hanada, K., Iwasaki, M., Yamaguchi, H., Ikeda, H.: "Illegitimate recombination induced by overproduction of DnaB helicase in Eschierichia coli."J. Bacteriol.. 181. 4549-4553 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nohnu, T., Suzuki, M., Masumura, K., Yamada, M., Matsui, K., Ueda, O., Suzuki, H., Katoh, M., Ikeda, H., Sofuni, T.: "Spi (-) selection : An efficient method to detect gamma-ray-induced deletions in transgenic mice."Environ. Mol. Mutagen. 34. 9-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Heo, S.-J., Tatebayashi, K., Ohsugi, I., Shimamoto, A., Furuichi, Y., Ikeda, H.: "Bloom's syndrome gene suppresses premature aging caused by Sgs1 deficiency in yeast."Genes Cells. 4. 619-625 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, H., Hanada, K., Asami, Y., Kato, I., Ikeda, H.: "Control of genetic stability in Escherichia coli the SbcB 3'-5' exonuclease suppresses illcgitimate recombination promoted by the RecE 5'-3' exonuclease."Genes Cells. 5. 101-109 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hanada, K., Iwasaki, M., Ihashi, S., Ikeda, H.: "UvrA and UvrB suppress illegitimate recombination : Synergistic action with RecQ helicase."Proc. Natl. Acad. Sci. USA. 97. 5989-5994 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kang, S.-W., Ikeda, H.: "WRN homologues in non-human systems. In Werner syndrome : Implications for aging and cancer."GANN MONOGRAPH ON CANCER RESEARCH. 49. 137-145 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onda, M., Ikeda, H.: "Role of DNA ligase in the illegitimate recombination that generates λbio transducing phages."Genetics. 158. 29-39 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shanado, Y., Hanada, K., Ikeda, H.: "Suppression of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS."Mol. Gen. Genet.. 265. 242-248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda, H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast."Nucleic Acid Res.. 29. 1326-1333 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatebayashi, K., Tani, T., Ikeda, H.: "Fission yeast Toilp, which interacts the small GTPase Ran, is required for mitosis-to-interphase transition and poly (A)^+ RNA metabolism."Genetics. 157. 1513-1522 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeda, T., Ogino, K., Tatebayashi, K., Ikeda, H., Arai, K., Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast."Mol. Biol. Cell.. 12. 1257-1274 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hanada, K., Yamashita, T., Shobuike, Y., Ikeda, H.: "Role of DnaB helicase in UV-induced illegitimate recombination in Escherichia coli."J. Bacteriol.. 183. 4964-4969 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kawabe, Y., Branzei, D.Hayashi, T., Suzuki, H., Masuko, T., Onoda, T., Heo, S.J., Ikeda, H., Shimamoto, A., Furuichi, Y., seki, M., Enomoto, T.: "A novel protein interacts with the Werner's syndrome gene product physically and functionally."J. Biol. Chem.. 276. 20364-20369 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Asami, Y., Jia, D.-W., Tatebayashi, K., Yamagata, K., Tanokura, M., Ikeda, H.: "Effect of DNA topoisomerase II inhibitor VP-16 on illegitimate recombination in yeast chromosome."Gene. 291. 251-257 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shiraishi, K., Hanada, K., Iwakura, Y., Ikeda, H.: "Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli."J. Bacteriol.. 181. 4715-4721 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sabourin, M., Nitiss, J.L., Nitiss, K.C., Tatebayashi, K., Ikeda, H., Osheroff, N.: "Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks."J. Mol. Biol.. 330. 29-42 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Janscak, P., Garcia, P.l., Hamburger, F., Makuta, Y., Shiraishi, K., Imai, Y., Ikeda, H., Bickle, T.A.: "Characterization and mutational analysis of the RecQ core of the Bloom Symdrome protein."Nucleic Acids Res.. 31. 4373-4384 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikeda, H., Shiraishi, K., Ogata, Y.: "Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli."Adv. Biophys. In press..

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shanado, Y., Hanada, K., Ikeda, H.: "Suppresion of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS"Mol. Gen. Genet.. 265. 242-248 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tatebayashi, K., Tani, T., Ikeda, H.: "Fission yeast Mog1p homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)^+ RNA metabolism"Genetics.. 157. 1513-1522 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kang, S.-W., Ikeda H.: "WRN homolgues in non-human systems. In Werner syndrome : Implications for aging and cancer"Gann Monograph on Cancer Reserch.. 49. 137-145 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Onda, M., Yamaguchi, J., Hanada, K., Asami, Y., Ikeda, H.: "Role of DNA ligase in the illegitimate recombination that generates λ bio transducing phages"Genetics. 158. 29-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeda, T., Ogino, K., Tatebayashi, K., Ikeda, H., Arai, K., Masai, H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast"Mol. Biol. Cell.. 12. 1257-1274 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda, H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome segregation in fission yeast"Nucleic Acid Res.. 29. 1326-1333 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hanada, K., Yamashita, T., Shobuike, Y., Ikeda, H.: "Role of DnaB belicase in UV-induced illegitimate recombination in E. coli"J. Bacteriol.. 183. 4964-4969 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawabe, Y., Branzei, D., Hayashi, T., Suzuki, H., Masuko, T., Onda, T., Heo, S.J., Ikeda, H., Shimamoto, A., Furuichi, Y., Seki, M., Enomoto, T.: "A novel protein interacts with the Werner's syndrome gene product physically and functionally"J. Biol. Chem.. 276. 20364-20369 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi,H.,Hanada,K.,Asami,Y.,Kato,J.and Ikeda,H.: "Control of genetic stability in Escherichia coli : the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease."Genes Cells. 5. 101-109 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hanada,K.,Iwasaki,M.,Ihashi,S.and Ikeda,H.: "UvrA and UvrB suppresses illegitimate recombination : Synergistic action with RecQ helicase."Proc.Natl.Acad.Sci.USA. 97. 5989-5994 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shanado,Y.,Hanada,K.and Ikeda,H.: "Suppression of gamma-ray-induced illegitimate recombination in Escherichia coli by the DNA binding protein H-NS."Mol.Gen.Genet.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Tatebayashi,K.,Tani,T.and Ikeda,H.: "Fission yeast Mog1p homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly (A)^+ RNA metabolism."Genetics. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Kang,S.-W.and Ikeda,H.: "WRN homologues in non-human systems. In Werner syndrome : Implications for aging and cancer."GANN MONOGRAPH ON CANCER RESEARCH. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Onda,M.and Ikeda,H.: "Role of DNA ligase in the illegitimate recombination that generates bio transducing phages."Genetics. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeda,T.,Ogino,K.,Tatebayashi,K.,Ikeda,H.,Arai,K.and Masai,H.: "Regulation of initiation of S phase, replication checkpoint signaling and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast."Mol.Biol.Cell.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Shobuike,T.,Tatebayashi,K.,Tani,T.,Sugano,S.and Ikeda,H.: "The dhp1^+ gene, encoding a putative nuclear 5'-3' exoribonuclease, is required for proper chromosome sygregation in fission yeast."Nucleic Acid Res.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Onda, M., Hanada, K., Kawachi, H. and Ikeda, H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress"Genetics. 151. 439-446 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ashizawa, Y., Yokochi, T., Ogata, Y., Shobuike, Y., Kato, J., and Ikeda, H.: "Mechanism of DNA gyrase-mediated illegitimate recombination : Characterization of Escherichia coli gyrA mutations the confer hyperrecombination phenotype"J. Mol. Biol.. 289. 447-458 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Heo, S.-J.,Tatebayashi, K. and Ikeda, H.: "The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC"Curr. Genet.. 36. 329-338 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamashita, T., Hanada, K., Iwasaki, M., Yamaguchi, H. and Ikeda, H.: "Illegitimate recombination induced by overproduction of DnaB helicase in Eschierichia coli"J. Bacteriol.. 181. 4549-4553 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nohmi, T., Suzuki, M., Masumura, K., Yamada, M., Matsui, K., Ueda, O., Suzuki, H., Katoh, M., Ikeda, H., Sofumi, T.: "Spi(-) selection : An efficient method to detect gamma-ray-induced deletions in transgenic mice"Environ. Mol. Mutagen. 34. 9-15 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Heo, S.-J., Tatebayashi, K. Ohsugi, I., Shimamoto, A., Furuichi, Y.and Ikeda, H.: "Bloom's syndrome gene suppresses premature aging caused by Sgs1 deficiency in yeast"Genes Cells. 4. 619-625 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi, H., Hanada, K., Asami, Y., Kato, J. and Ikeda, H.: "Control of genetic stability in Escherichia coli : the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease"Genes Cells. 5. 101-109 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kang, S.-W. and Ikeda, H.: "Werner syndrome : Implications for aging and cancer. RN homologues in non-human systems"GANN MONOGRAPH ON CANCER RESEARCH.. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Fukami, A., Taniguchi, Y., Nakamura, T., Rho, M. C., Kawaguchi, K., Hayashi, M., Kohiyama, K., and Omura, S.: "New members of macrosphelides from Microsphaeropsis sp. Fo-5050IV"J. Antibiot. (Tokyo). 52. 501-504 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Rho, M. C., Toyoshima, M., Hayashi, M., Kayano, T., Subramaniam, G., Kam, T. S., and Kohiyama, K.: "Reversal of multidrug resistance by kopsiflorine isolated from Kopsia dasyrachis"Planta Med.. 65. 307-310 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inokoshi, J., Katagiri, M., Arima, S., Tanaka, H., Hayashi, M., Kim, Y. B., Furumai, R., Yoshida, M., Horinouchi, S., and Omura, S.: "Neuronal differenciation of Neuro 2a cells by inhibitors of cell cycle progression, trichostain A and butyrolactone 1"Biochem. Biophys. Res. Commun.. 256. 372-376 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Komano, Y., Zhang, H.-P., Hiraide, H., Kotake, A., Hashima, H., Kizu, H., Komiyama, K., Hayashi, M., and Pettit, G. R.: "Lutamide A-C, three new b-phenylethylamine alkaloids from marine Bryozoa Ama thia convoluta"Chemistry Letters,. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Heo, S-J., Tatebayashi, K., Kato, J., and Ikeda,H.: "Budding yeast RHC21 gene, a homologoue of fisslon yeast rad21^+ gene, is essential for chromosome segregation." Mol.Gen.Genet.257. 149-156 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamagata, k., Kato, J., Shimamoto, A., Goto, M., Furuichi, Y., and Ikada ,H.: "Bloom's and Wemer's syndrome genes suppress hyperrecombination in yeast sgsI mutant Implication for genomic instability in human diseases." Proc.Nati.Acad.Sci.USA. 95. 8733-8738 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato, Y., Shimamoto, A., Shobuike, T., Sugimoto, M., Ikeda, H., Kuroda, S.and Furiichi, Y.: "Cloning and characterization of human Sep1 (hSEP1) gene and cytoplasmic localization of its product." DNA Res.5. 241-246 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shanado, Y., Kato, J.and Ikeda, H.: "Escherichia coll HU protein suppresses DNA-gyrase-mediated illegitimate recombination and SOS induction." Genes Cells. 3. 511-520 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsukamoto, Y.and Ikeda, H.: "Double-strand break repair mediated by DNA end-joining." Genes Cells. 3. 135-144 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Onda, M., Hanada, K., Kawachi, H.and Ikeda, H.: "Escherichia coil MutM suppresses illegitimate recombination induced by oxidative stress." Genetics. 151. 439-446 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tatebayashi, K., Kato, J., and Ikeda, H.: "Isolation of a Schizosac charomyces pom be rad21ts muatnt which is aberrant in chromosome segregation, microtubule function, DNA repair and DNA replication : possible involvement of Rad21 in ublqultin-medlated proteolysis." Genetics. 148. 49-57 (19919988)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Hasebe, S.Tsushima and S.Iida: "Isolation and charecterization of IS1416 from Pseudomonas glumae, an new member of the IS3 Familly" Plasmid. 39. 196-204 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Iida: "1998 Accessory genes in the darA operon of bacterlophage P1 affect anti-restriction function, generalized transduction, head morphogenesis and host cell lysis" Virology. 251. 49-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Habu, Y.Hisatomi and S.Iida: "Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory." Plant J.16. 371-376 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Inagaki,: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories" Gene. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Takahashi,: "Capturing of a genomic HMG domain sequence by an En-Spm related transposable element Tpn1 in the Japanese morning glory" Mol.Gen.Genet.(in press).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2018-03-28  

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