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Transposition mechanisms and biotechnology application of the medaka fish To12 transposable element

Research Project

Project/Area Number 10216205
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionGrant-in-aid for Scientific Research on Priority Areas (2)

Principal Investigator

KOGA Akihiko  Nagoya University, Graduate School of Science, Associate professor, 大学院・理学研究科, 助教授 (80192574)

Project Period (FY) 1998 – 2002
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥28,000,000 (Direct Cost: ¥28,000,000)
Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2001: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2000: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1999: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1998: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsMedaka fish / Mobile genetioc element / Transposable element / Tol2 element / Transposition mechanisms / Gene transfer / Transposase / 転移因子 / 挿入 / Tol2 / 変異 / 自然集団
Research Abstract

The first three years of the five-year research term were devoted to basic research on the transposition mecchanisms of the Tol2 element. The following results were obtained : (1)Portions of the 4.7-kb element esstential for the transposition reaction were detected. (2)An autonomous Tol2 copy, carrying a gene for the transposase, was identified. (3)An mRNA for the transposase was isolated from medaka fish cells. (4)De novo insertion in medeka fish cells was detected, with a newly devised experimental system including several bacterial drug-resistance genes. (5)Target site duplications were proved to be exactly 8 bp with no preference for specific sequences or nucleotides.
In the fourth year, the research was expanded to include biotechnology applications. The results were as follows : (1)Addition of a nuclear localization signal to the transposase led to a three times higher transposition frequency. (2)A Tol2 copy carrying the GFP gene as a marker gene was successfully integrated into the medaka fish chromosomes. (3)The integration occurred in the germline cells, and the inserted copy was inherited to subsequent generations. (4)Gene transfer was also successful in zebrafish. (5)Tol2 was demonstrated to transpose in human and mouse cells in the same manner as that in fish. (6)An extranuclear localization signal was suggested to be present in the transposase protein. (7)One main signal was suggested to be present at about the center of the primary structure of the transposase protein. (8)X-ray irradiation was found to induce transposition at a high frequency.

Report

(6 results)
  • 2003 Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Iida A, Inagaki H, Suzuki M, Hori H, Koga A: "The tyrosinase gene of the i^b albino mutant of the medaka fish carries a transposable element insertion in the promoter region."Pigment Cell Research. 17(2)(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsutsumi M, Koga A, Hori H: "Long and short mRNAs from the medaka fish transposon Tol2 resepectively exert positive and negative effects on excision."Genetical Research. 82(1). 33-40 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Iida A, Kamiya M, Hayashi R, Hori H, Ishikawa Y: "The medaka fish Tol2 transposable element can undergo excision in human and mouse cells.The medaka fish Tol2 transposable element can undergo excision in"Journal of Human Genetics. 48(5). 321-325 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Hori H, Sakaizumi M: "Gene transfer and cloning of flanking chromosomal regions using the medaka fish Tol2 transposable element."Marine Biotechnology. 4(1). 6-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Hori H: "Detection of de novo insertion of the medaka fish transposable element Tol2."Genetics. 156(3). 1243-1247 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Suzuki M, Maruyama Y, Tsutsumi M, Hori H: "Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2."FEBS Letters. 461(3). 295-298 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iida A, Inagaki H, Suzuki M, Hori H, Koga A: "The tyrosinase gene of the i^b albino mutant of the medaka fish carries a transposable element insertion in the promoter region."Pigment Cell Research. 17(2). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsutsumi M, Koga A, Hori H: "Long and short mRNAs from the medaka fish transposon Tol2 resepectively exert positive and negative effects on excision."Genetical Research. 82(1). 33-40 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Iida A, Kamiya M, Hayashi R, Hori H, Ishikawa Y, Tachibana A: "The medaka fish Tol2 transposable element can undergo excision in human and mouse cells."Journal of Human Genetics. 48(5). 231-235 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Hori H, Sakaizumi M: "Gene transfer and cloning of flanking chromosomal regions using the medaka fish Tol2 transposable element."Marine Biotechnology. 4(1). 6-11 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Hori H: "Detection of de novo insertion of the medaka fish transposable element Tol2."Genetics. 156(3). 1243-1247 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koga A, Suzuki M, Maruyama Y, Tsutsumi M, Hori H: "Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2."FEBS Letters. 461(3). 295-298 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] KOGA, Akihiko: "The medaka fish Tol2 transposable element can undergo excision in human and mouse cells"Journal of Human Genetics. 48(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] KOGA, Akihiko: "Gamera, a family of LINE-like repetitive sequences widely distributed in medaka and related fishes"Heredity. 89(6). 446-452 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] KOGA, Akihiko: "Gene transfer and cloning of flanking chromosomal regions using the medaka fish Tol2 transposable element"Marine Biotechnology. 4(1). 1-4 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] KOGA, Akihiko: "Transposable elements in medaka fish"Zoological Science. 19(1). 1-6 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] KOGA,Akihiko: "Detection of de novo insertion of the medaka fish transposable element Tol2."Genetics. 156(3). 1243-1247 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 古賀,章彦: "メダカ属でのトランスポゾンの進化"蛋白質核酸酵素. 45(17). 2900-2908 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] KOGA,Akihiko: "The Tol2 transposable element of the medaka fish : an active DNA-based element naturally occurring in a vertebrate genome."Genes & Genetic Systems. 156(3). 1243-1247 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] KOGA, Akihiko: "Oculocutaneous albinism in the I^6 mutant of the medaka fish is associated with a deletion in the tyrosinase gene"Pigment Cell Research. 12(4). 252-258 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] KOGA, Akihiko: "Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2"FEBS Letters. 461(3). 295-298 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] KOGA, Akihiko: "Evidence for recent invasion of the medaka fish genome by the Tol transposable element"Genetics. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] KAWAKAMI,Koichi: "Excision of the Tol2 transposable element of the medaka fish,Oryzias latipes,in zebrafish,Danio rerio." Gene. 225(1). 17-22 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] INAGAKI,Hidehito: "The tyrosinase gene from medakafish:Transgenic expression rescues albino mutation." Pigment Cell Research. 11(5). 283-290 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] KOGA,Akihiko: "Homogeneity in the structure of the medaka fish transposable element Tol2." Genetical Research. 73(1). 7-14 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2018-03-28  

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