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脳・神経系形成における誘導のメカニズムの解明

Research Project

Project/Area Number 10220101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTohoku University

Principal Investigator

仲村 春和  東北大学, 大学院・生命科学研究科, 教授 (90079690)

Co-Investigator(Kenkyū-buntansha) 田中 英明  熊本大学, 大学院・医学研究科, 教授 (90106906)
岡本 仁  理化学研究所, 脳科学総合研究センター, チームリーダー (40183769)
影山 龍一郎  京都大学, ウイルス研究所, 教授 (80224369)
笹井 芳樹  理化学研究所, 発生・再生科学総合研究センター, グループディレクター (20283616)
武田 洋幸  東京大学, 大学院・理学系研究科, 教授 (80179647)
野田 昌晴  岡崎国立共同研究機構, 基礎生物学研究所, 教授 (60172798)
村上 富士夫  大阪大学, 大学院・基礎工学研究科, 教授 (20089882)
藤澤 肇  名古屋大学, 大学院・理学系研究科, 教授 (60079689)
Project Period (FY) 1998 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥32,600,000 (Direct Cost: ¥32,600,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2002: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2000: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1998: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywords脊椎動物 / 神経管 / 領域化 / 前脳 / 峡部 / オーガナイザー / zli / 間脳 / 脳胞 / 領域 / Fgf8 / Islet3 / ES細胞 / ドパミン産生細胞 / Hes / 嗅球 / 脳 / 誘導 / パターン形成 / 回路形成 / トランスジェニック / ノックアウト / 遺伝子強制発現 / 中枢神経系 / コンパートメント / 位置特異性 / 分化 / 増殖 / 神経回路形成
Research Abstract

「脳のパターン形成研究」班は平成10-15年の6年にわたって、最新の分子生物学的手法、遺伝子改変のテクニックなどを駆使して、脊椎動物の脳・神経系の形態形成に焦点を当て手研究を行ってきた。本研究プロジェクトでは、特に(1)発生初期の神経としての分化の決定、(2)その後中枢神経内でのコンパートメントの形成、(3)コンパートメント内での位置特異性の決定、(4)神経回路の形成の機構についての各班員が分担して研究を行った。
本研究領域は6年間にわたり展開され、これまでの研究成果の項に記すように各研究班ともに成果をあげている。そこで本研究領域の成果をとりまとめ広く公表するとともに、今後の展開、共同研究の道を開くため公開シンポジウムを開催する。
本年度はその成果公開のため国際公開シンポジウムを開催した。シンポジウムには海外からMarion Wassef, Andrea Wizenmann, Elizabeth Grove博士を招待し、国内講演者は本研究班の班員を中心とし、関連の研究者を加え、13人の演者による発表が行われた。シンポジウムでは、脊椎動物脳のパターン形成に関して様々な視点からの講演と討論が行われ、これまでの各演者の成果を交換するとともに今後の研究の展開、共同研究の可能性についても意見が交換された。

Report

(7 results)
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (50 results)

All 2004 Other

All Journal Article (6 results) Publications (44 results)

  • [Journal Article] The Fgf8 signal causes cerebellar differentiation by activating Ras-ERK signaling pathway.2004

    • Author(s)
      T.Sato, H.Nakamura
    • Journal Title

      Development 131

      Pages: 4275-4285

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Misrouting of mitral cell progenitors in the Pax6/small eye rat telencephalon.2004

    • Author(s)
      Nomura, T., Osumi, N
    • Journal Title

      Development 131

      Pages: 787-796

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The neurotrophin-receptor-related protein NRH1 is essential for convergent extension movements.2004

    • Author(s)
      Sasai N, Nakazawa Y, Haraguchi T, Sasai Y.
    • Journal Title

      Nat.Cell Biol 6

      Pages: 741-748

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Instability of Hes7 protein is crucial for the somite segmentation clock.2004

    • Author(s)
      Hirata H, Bessho Y, Kokubu H, Masamizu Y, Kageyama R他2名
    • Journal Title

      Nat Genet. 36

      Pages: 750-754

    • Related Report
      2004 Annual Research Report
  • [Journal Article] PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching.2004

    • Author(s)
      Miyashita T, Yeo SY, Hirate Y, Segawa H, Okamoto H.他4名
    • Journal Title

      Development 131

      Pages: 3705-3715

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential activities of Sonic hedgehog mediated by Gli transcription factors define distinct neuronal subtypes in the dorsal thalamus.2004

    • Author(s)
      Hashimoto-Torii K, Motoyama J, Hui CC, Shimamura K.他2名
    • Journal Title

      Mech.Devel. 120

      Pages: 1097-1111

    • Related Report
      2004 Annual Research Report
  • [Publications] Watanabe, Y., Toyoda, R., Nakamura, H.: "Navigation of Trochlear axons along the midbrain hindbrain boundary to neuropilin-2."Development. 131. 681-692 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nomura, T., Osumi, N.: "Mis-routing of mitral cells in Pax6/Small eye rat telencephalon."Mis-routing of mitral cells in Pax6/Small eye rat telencephalon.. 131・4. 787-796 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masai, I., Okamoto, H., 他10人: "N-cadherin mediates retinal lamination, maintenance of forebrain compartments and patterning of retinal neurites."Development. 130・11. 2479-2494 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakamoto, M., Ohtsuka, T., Bessho, Y., Kageyama, R.: "The bHLH genes Hesr1/Hey1 and Hesr2/Hey2 regulate maintenance of neural precursor cells in the brain."J.Biol.Chem.. 278. 44808-44815 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuta, H., Kanai, M., Ito, Y., Yamasu, K.: "gbx2 homeobox gene is required for the maintenance of the isthmic region in the zebrafish embryonic brain."Dev.Dyn.. 228. 433-450 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizuseki, K., Sasai, Y., 他10人: "Generation of Neural Crest-Derived PNS Neurons and Floor Plate Cells from Mouse and Primate ES Cells."Proc.Natl.Acad.Sci., USA. 100. 5828-5833 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 仲村春和, (伊藤正男監修, 金澤一郎, 篠田義一, 広川信隆, 御子柴克彦, 宮下保司編): "脳神経科学"三輪書店. 841 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsunaga, E., Katahira, T., Nakamura, H.: "Role of Lmx1b and Wnt1 in mesencephalon and metencephalon development"Development. 129. 5269-5277 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, M., Osumi, N.: "Pax6 regulates specification of ventral neuron subtypes in the hindbrain by establishing progenitor domains"Development. 129. 1327-1338 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirata, H., Yoshiura, S., Ohtsuka, T., Bessho, Y., Harada, T., Yoshikawa, K., Kageyama, R.: "Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop"Science. 298. 840-843 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tsuda, H., Sasai, N., Matsuo-Takasaki, M., Sakuragi, M., Murakami, Y., Sasai, Y.: "Dorsalization of the Neural Tube by Xenopus Tiarin, a Novel Patterning factor Secreted by the Flanking Non-Neural Head Ectoderm"Neuron. 33. 515-528 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi, D., Kobayashi, M., Matsumoto, K., Ogura, T., Nakafuku, M., Shimamura, K.: "Early subdivisions in the neural plate define distinct competence for inductive signals"Development. 129. 83-93 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Taniguchi, M., Nagao, H., Takahashi, Y.K., Yamaguchi, M., Mitsui, S., Yagi, T., Mori, K., Shimizu, T.: "Distorted odor maps in the olfactory bulb of semaphorin 3A-deficient mice"Journal of Neuroscience. 23. 1390-1397 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamoto, H., Segawa, H., Higashijima, S.: "Aquatic Genome-Steps toward a Great Future"Springer-Verlag. (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, T., Araki, I., Nakamura, H.: "Inductive signal and tissue responsiveness to difine the tecturn and the cerebellum"Development. 128. 2461-2469 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Inoue, T., Tanaka, T., Takeichi, M., Chisaka, O., Nakamura, S., Osumi, N.: "The role of cadherins in maintaining the compartment boundary between the cortex and striatum during development"Development. 128. 561-569 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ando, H., Furuta, T., Tsien R.Y., Okamoto, H.: "Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos"Nature Genet.. 28. 317-325 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata, H., Tomita, K., Bessho, Y., Kageyama, R.: "Hes 1 and Hes 3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain"EMBO J.. 20. 4454-4466 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sasai, N., Mizuseki, K., Nagata, K., Sasai, Y.: "Requirement of Fox D3-class Signaling for Neural Crest Determination in Xenopus"Development. 128. 2525-2536 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kobayashi, D., Kobayashi, M., Matsumoto, K., Ogura, T., Nakafuku, M., Shimamura, K.: "Early subdivisions in the neural plate define distinct competence for inductive signals"Development. 129. 83-93 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 武田洋幸: "動物のからだづくり・形態発生の分子メカニズム・シリーズ応用動物科学・バイオサイエンス2"朝倉書店. (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura,H.: "Regionalisation of the optic tectum : Combination of the gene expression that defines the tectum."Trends Neurosci.. 24. 32-39 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yeo,S.-Y.,Little,M.H.,Yamada,T.,Miyashita,T.,Halloran M.C,Kuwada,J.Y.,Huh,T.-L.Okamoto,H.: "Overexpression of a slit Homologue Impairs Convergent Extension of the Mesoderm and Causes Cyclopia in Embryonic Zebrafish."Devel.Biol.. 230. 1-17 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomita,K.,Moriyoshi,K.,Nakanishi,S.Guillemot,F.,and Kageyama,R.: "Mammalian achaete-scute and atonal homologs regulate neuronal versus glial fate determination in the central nervous system."EMBO J.. 19. 5460-5472 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawasaki,H.,Mizuseki,K.,Nishikawa,S.,Kaneko,S.,Kuwana,Y.,Nakanishi,S.,Nishikawa,Sl.,Sasai,Y.: "Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity."Neuron.. 28. 31-40 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uchida,N.,Takahashi,Y.K.,Tanifuji,M.Mori,K.: "Odor maps in the mammalian olfactory bulb : domain organization and odorant structural features."Nature Neurosci.. 3. 1035-1043 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Inoue,T.,Nakamura,S.,Osumi,N.: "Fate mapping of the mouse prosencephalic neural plate."Dev.Biol.. 219. 373-383 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 岡本仁(森,真壁,渡辺,岡野,宮川 編): "神経系の成り立ち-神経誘導とパターン形成、神経科学イラストレイテッド "羊土社. 92-106 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大隅典子,中福雅人(岩田誠 編): "Moleculer Medicine増刊号「ゲノム時代の脳神経医学」第3章4)脳の領域特異性獲得のメカニズム"中山書店. 218-225 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] I. Araki, H. Nakamura: "Engrailed defines the position of dimesencephalic boundary by repressing diencephalic fate"Development. 126. 5127-5135 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. kishi, K. Mizuseki, H. Yamazaki, K. Shiota, S. Nakanishi, Y. Sasaki: "Requirement of Sox2-mediated signaling for differentiation of early Xenopus neuroectoderm"Development. 127. 791-800 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Mizuno, E. Yamaha, A. Kuroiwa, H. Takeda: "Removal of vegetal yolk causes dorsal deficiencies and impairs dosal inducing ability of the yolk cell in zebrafish"Mech. Dev.. 81. 51-63 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Higashijima, Y. Hotta, H. Okamoto: "Visualization of cranial motor neurons in live transgenic zebrafish expressing GFP under the control of the Islet-l promoter lenbancer"J. Neurosci. 20. 206-218 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Mori, H. Nagao, Y. Yoshihara: "The Olfactory Bulb : Coding and Processing of Odor Molecule Information"Sience. 286. 711-715 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Konishi, N. Ohkawa, Y. Makino, H. Ohkubo, R. Kageyama, T. Furuichi, K. mikoshiba, T.Tomura: "Transcriptional regulation of mouse type1 inositol 1, 4, 5-triphosphate receptor gene by NeuroD-reloted factor"J. Neurochem.. 72. 1717 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 仲村春和(生塩之敬,山浦晶 編): "神経堤由来組織の腫瘍性病変"三輪書店. 136 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岡本仁(金子章道,川村光毅,植村慶一 編): "分子神経生物科学入門-脳と神経-"共立出版株式会社. 363 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.sugiyama,J.Funahashi,J.Kitajewski H.Nakamura: "Crossregulation between En-2 and Wnt-1 in chick tectal development." Develop.Growth Differ.40. 157-166 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Funahashi,T.Okafuji,H.Ohuchi S.Noji,H,Tanaka,H.Nakamura: "Role of Pax-5 in the regulation of a mid-hindbrain organizer's activity." Develop.Growth Differ.41(1). 59-72 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Tsuda,K.Takebayashi,S.Nakanishi R.Kageyama: "Structure and Promoter Analysis of Math3 Gene,and Mouse Homolog of Drosophila Proneural Gene atonal." The Journal of Biological Chemistry. 273(11). 6327-6333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Higashijima,H.Okamoto,N.Ueno,Y.Hotta,G.Eguchi: "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using prooter of zebrafish origin." Dev.Biol.192. 289-299 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Higashijima,A.Nose,G.Eguchi Y.Hotta,H.Okamoto: "Mindin/F-spondin family:novel ECM proteins expressed in the zebrafish embryonic axis." Dev.Biol.192. 211-227 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 仲村春和(石川春律他編): "標準細胞生物学" 医学書院, 361 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Larsen(仲村春和他訳): "ラーセン「最新人体発生学」第2版" 西村書店, 504 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2018-03-28  

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