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Structure, mechanism of formation and genetic regulation of associations between lignin and polysaccharides in plant cell walls

Research Project

Project/Area Number 10306011
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionThe University of Tokyo

Principal Investigator

IIYAMA Kenji  Asian Natural Environmental Science Center, The University of Tokyo, Professor, アジア生物資源環境研究センター, 教授 (60012077)

Co-Investigator(Kenkyū-buntansha) KOJIMA Katsumi  Asian Natural Environmental Science Center, The University of Tokyo, Associate Professor, アジア生物資源環境研究センター, 助教授 (80211895)
MATSUMOTO Yuji  Graduate School of Agricultural and Life Sciences, The University of Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30183619)
KATAYAMA Yoshihiro  Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Associate Professor, 大学院・生物システム応用科学研究科, 助教授 (10214339)
SHINTANI Hiroyuki  Graduate School of Agricultural and Life Sciences, The University of Tokyo, Research Fellow, 大学院・農学生命科学研究科, 助手 (30282693)
ISHII Tadashi  Independent Administrative Institution, Forestry and Forest Products Research Institute, Division of Tree Biochemistry, Head of Division, Researcher, 生物機能開発部, 研究室長(研究職) (90353573)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥36,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2001: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥20,700,000 (Direct Cost: ¥20,700,000)
KeywordsPlant Cell Wall / Associations between Lignin and Polysaccharides / Cellulose / Lignin / Bridges through Ferulic Acid / Rhamnogalacturonan / Periodate Oxidation / Bjorkman LCC / Bjorkman LCC / リグニン・多糖結合 / DDQ酸化
Research Abstract

The associations between lignin and polysaccharides are the subjects of much study in plant science, but the economic impact of the associations is not well recognised. Residual lignins linked firmly to polysaccharides in chemical pulp for paper making are removed by bleaching using chlorine or chlorine dioxide. Toxic and mutagenic chlorinated chemicals such as doxin are released into ecosystems from bleaching system. Lowering of nutritive values of forage plants during maturation is probably due to the formation of associations between lignin and polysaccharides. It is important, therefore to elucidate the biological, biochemical and chemical features of the associations.
Lignified secondary walls of any vascular plants have associations between lignin and polysaccharides of different extent and with different types of linkages depending on the plant species. The associations visualized by chemical substitution of walls suggest that the important parts of plant organs are reinforced mechanically by the associations. Lignin macromolecules are flexible, and can cover the surface of polysaccharide fibrils and freely entangle with the polysaccharide matrix, as well as forming covalent linkages. Current understanding of the linkage types between lignin and polysaccharides is reviewed, with particular, attention to the chemical and macromolecular features of lignin cell walls of plants.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (55 results)

All Other

All Publications (55 results)

  • [Publications] Iiyama, K., Lam, T.B.T.: "Structural characteristics of cell walls of forage grasses-Their nutritional evaluation for ruminants-a Review"Asian Austr. J. Anim. Sci.. 14. 862-879 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lam, T.B.T., Kadoya, K., Iiyama, K.: "Bonding of hydroxycinnamic acids to lignin-ferulic and p-coumaric acids bind at the benzyl position of lignin, not the β-position in grass cell walls"Phytochemistry. 57. 987-992 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 飯山賢治: "生物資源としての里山の可能性"里山の環境学(東京大学出版会). 173-182 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakegawa K, Edashige Y, Ishii T.: "Metabolism of cell wall polysaccharides in cell suspension cultures of Populus alba in relation to cell growth"Physiol. Plant.. 108. 420-425 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Baba, Y., Ishida, Y., Oda, M., Iiyama, K., Akita, S.: "Decomposition of (1-3,1-4)-a-glucan and expression of the (1-3,1-4)-a glucanase gene in rice stems during ripening"Plant Prod. Sci.. 4. 230-234 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshida-S.T, Yoshida S, Kakegawa K, Ishii T.: "Enzymic feruloylation of arabinoxylan-trisaccharide by feruloyl-CoA : arabinoxylan-trisaccharide O-hydroxycinnamoyl transferase from Oryza sativa"Planta. 212. 470-474 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishii T, Matsunaga T, Hayashi N.: "Formation of rhamnogalacturonan II-borate dimer in pectin determines cell wall thickness of pumpkin tissue"Plant Physiol. 126. 1698-1705 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwai H, Ishii T, Satoh S.: "Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolla non-organogenic callus with loosely attached constituent cells"Planta. 213. 907-915 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakurai N, Katayama Y, Yamaya T.: "Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice"Physiol. Plant.. 113. 400-408 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iiyama, K. and Lam, T.B.T.: "Structural characteristics of cell walls of forage grasses - Their nutritional evaluation for ruminants - a Review"Asian Austr. J. Anim. Sci.. 14. 862-879 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lam, T.B.T., Kadoya, K. and Iiyama, K.: "Bonding of hydroxycinnamic acids to lignin - ferulic and p-coumaric acids bind at the benzyl position of lignin, not the β-position in grass cell walls"Phytochemistry. 57. 987-992 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iiyama, K.: "Possibility of Satoyama and Biomass Supply""Satoyama no Kankyougaku" (SATOYAMA: The Traditional Rural Landscape of Japan) Eds. Takeuchi, K., Washitani, I., Tsunekawa, A.. 173-182 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kakegawa, K., Edashige, Y., Ishii, T.: "Metabolism of cell wall polysaccharides in cell suspension cultures of Populus alba in relation to cell growth"Physiol. Plant. 108. 420-425 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Baba, Y., Ishida, Y., Oda, M., Iiyama, K., and Akita, S.: "Decomposition of (1-3, 1-4)-aglucan and expression of the (1-3,1-4)-aglucanase gene in rice stems during ripening"Plant Prod. Sci.. 4. 230-234 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshida-S., T., Yoshida S., Kakegawa K., Ishii T.: "Enzymic feruloylation of arabinoxylan-trisaccharide by feruloyl-CoA: arabinoxylan-trisaccharide O-hydroxycinnamoyl transferase from Oriza Sativa"Planta. 212. 470-474 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishii, T., Matsunaga, T., Hayashi, N.: "Formation of rhamnogalactoronan II-borate dimer in pectin determines cell wall thickness of pumpkin tissue"Plant Physiol.. 126. 1698-1705 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iwai, H., Ishii, T., Satoh, S.: "Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolla non-organogenic callus with loosely attached constituent cells"Planta. 213. 907-915 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakurai, N., Katayama, Y., Yamaya, T.: "Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice"Physiol. Plant. 113. 400-408 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miwa M., Tanaka, R., Yamanoshita, T., Norisada, M., Kojima K., Hogetsu T.: "Analysis of clonal structure of Melaleuca cajuputi (Myrtaceae) at a barren sandy site in Thailand using microsatellite polymorphism"Trees-Struct. Function. 15(4). 242-248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miwa, M., Tanaka, R., Shinone, M., Kojima, K., Hogetsu T.: "Development of polymorphic microsatellite markers in a tropical tree species, Melaleuca cajuputi (Myrtaceae)"Mol. Ecol.. 9. 639-640 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishikubo, N., Araki, T., Kajita, S., Kuroda, K., Kitano, H., Katayama, Y.: "Specific accumulation of polysaccharide-linked hydroxycinnamoyl esters in the cell walls of irregularly shaped and collapsed internode parenchyma cells of the dwarf rice mutant Fukei 71"Plant Cell Physiol.. 41. 776-784 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Lam, T.B.T., Iiyama, K.: "Characteristics of senescent straw cell walls of dwarf, semidwarf, and normal strains of rice (Oryza sativa) plants"J. Wood Sci.. 46. 376-380 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Karrison, O., Ikeda, T., Kishimoto, T., Magara, K., Matsumoto, Y., Hosoya S.: "Ozonation of a lignin-carbohydrate complex model compound of the benzyl ether type"J. Wood Sci.. 46. 263-265 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimokawa T., Ishii T., Matsunaga T.: "Isolation and structural characterization of rhamnogalacturonan II-borate complex from Pinus densiflora"J. Wood Sci.. 45. 435-439 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iiyama, K., Lam, T.B.T.: "Structural characteristics of cell walls of forage grasses-Their nutritional evaluation for ruminants- a Review"Asian Austr. J. Anim. Sci.. 14. 862-879 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Lam, T.B.T., Kadoya, K., Iiyama, K.: "Bonding of hydroxycinnamic acids to lignin-ferulic and p-coumaric acids bind at the benzyl position of lignin, not the β-position in grass cell walls"Phytochemistry. 57. 987-992 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 飯山賢治: "生物資源としての里山の可能性"「里山の環境学」、東京大学出版会. 173-182 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kakegawa K, Edashige Y, Ishii T.: "Metabolism of cell wall polysaccharides in cell suspension cultures of Populus alba in relation to cell growth"Physiol. Plant.. 108. 420-425 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Baba, Y., Ishida, Y., Oda, M., Iiyama, K., Akita, S.: "Decomposition of (1-3, 1-4)-a glucan and expression of the (1-3, 1-4)-a glucanase gene in rice stems during ripening"Plant Prod. Sci.. 4. 230-234 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshida-S.T, Yoshida S, Kakegawa K, Ishii T.: "Enzymic feruloylation of arabinoxylan-trisaccharide by feruloyl-CoA : arabinoxylan-trisaccharide O-hydroxycinnamoyl transferase from Oryza sativa"Planta. 212. 470-474 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishii T, Matsunaga T, Hayashi N.: "2001. Formation of rhamnogalacturonan II-borate dimer in pectin determines cell wall thickness of pumpkin tissue"Plant Physiol. 126. 1698-1705 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Iwai H, Ishii T, Satoh S.: "Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolla non-organogenic callus with loosely attached constituent cells"Planta. 213. 907-915 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakurai N, Katayama Y, Yamaya T.: "2001. Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice"Physiol. Plant.. 113. 400-408 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ishii,H.Ono,: "NMR spectroscopic analysis of the borate diol esters of methyl apiofuranosides"Carbohydrate Research. 321. 257-260 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Shimokawa,T.Ishii and T.Matsunaga: "Isolation and structural characterization of rhamnogalacturonan II-borate complex from Pinus densiflora,"Journal of Wood Science. 45. 435-439 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Ishii,T.Matsunaga,A.Darvill and P.Albersheim: "The plant cell wall polysaccharide rhamnogalacturonan II self-assembles into a covalently cross-linked dimer.,(1999)"Journal of Biological Chemistry. 274(9). 13098-13104 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Kakegawa,Y.Edashige and T.ishii.: "Metabolism of cell wall polysaccharides in cell suspension cultures of Populus alba L.in relation to cell growth."Physiolgica.Plantarum. 108. 420-425 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Edashige and T.Ishii: "Extracellular polysaccharides from the suspension-culture of Populus alba L."Holzforschung,(1999). 53. 137-141 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hori,K.,Flavier,M.E,Lam,T.B.T.and Iiyama K.: "An excellent oil absorbent, Kapok (Ceiba pentandra (L.) Gaertn.) fiber : its fiber structure, chemical characteristics and application."Journal of Wood Science. 46. 401-404 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Lam,T.B.T.and Iiyama,K.: "Characteristics of Senescence Straw Cell Walls of Dwarf, Semidwarf and Normal Strains of Rice (Oryza sativa) plants."Journal of Wood Science. 46. 376-380 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Iiyama,K.and Lam,T.B.T.: "Structural characteristics of cell walls of forage grasses-Their nutritional evaluation for ruminants-a Review."Asian Austrasian Journal of Animal Science in print. 14(4)(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Lam T.B.T.,Kadoya,K.and liyama,K.: "Bonding of hydroxycinnamic acids to lignin-ferulic and ρ-coumaric acids bind at the benzyl position of lignin, not the β-position in grass cell walls."Phytochemistry. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujimoto, A., Meshitsuka, G., and Iiyama, K.: "Covalent associations between lignin and cellulose as a major lignin and polysaccharide linkage"Proc. 10^<th> International Symposium on Wood & Pulping Chemistry 1999. 2. 48-51 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Lam, T. B. T., Kadoya, K. and Iiyama, K.: "Bonding of hydroxycinnamic acids to lignin-ferulic and p-coumaric acids bind at the benzyl oosition of lignin, not the β-position in grass cell walls"Phytochemistry. 53(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 樋口暁浩、飯山賢治、新城健: "熱帯産イネ科植物細胞壁構造と消化性の関係"ルーメン研究会報. 10. 37-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iiyama, K.: "Biomass Industrial Complex using wastes from tropical plantations"Targeting Zero Emissions for the Utilization of Renewable Resoueces. United Nations University, Tokyo. 20-29 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Shimokawa, T. Ishii and T. Matsunaga: "Isolation and structurasl characterization of rhamnogalacturonan II-borate complex from Pinus densiflora"J. Wood Sci.. 45. 435-439 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Ishii and H. Ono: "NMR spectroscopic analysis of the borate diol esters of methyl apiofuranosides"Carbohydr. Res.. 321. 257-260 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Ishii, T. Matsunaga, P. Pellerrin, M. O'Neill, A. Darvill and P. Albersheim: "Trhe plant cell wall polysaccharide rhamnogalacturonan II self-assembles into a covalently cross-linked dimer"J. Biol. Chem.. 274. 12098-13104 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iiyama, K., Hori, K., Flavier, M. E. and Lam, T. B. T.: "Excellent oil absorbent, Kapok(Ceiba pentandra(L.) Gaeth.) fiber : its fiber structure, chemical characteristics and its application"Proc. 10^<th> International Symposium on Wood & Pulping Chemistry, Yokohama, June 1999. 3. 496-499 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishimura, T.: "Structure of neutral branched xylooligosaccharides produced by xylanase from in situ reduced hardwood xylan" Carbohydrate Research. 308(1-2). 117-122 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki, S.: "Compositional and structural characteristics of residual biomass from tropical plantations." Journal of Wood Science. 44(1). 40-46 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki, S.: "Preparation of binderless boards from steam exploded pulps of oil palm (Elaeis guneensis Jaxq. ) fronds and structural characteristics of lignin and wall polysaccharides in steam exploded pulps to be discussed for self-bindings." Holzforschung. 52(4). 417-426 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 朴 承榮: "イネワラのアルカリ系蒸解における脱シリカ反応に影響する因子" 紙パ技協誌. 53(2). 214-221 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯山賢治: "地球環境学第7巻「未利用木質資源の再資源化」" 岩波書店, 41 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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