| Project/Area Number |
10306017
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
YAMAMOTO Yoshio Hiroshima University, Faculty of Applied Biological Science, Professor, 生物生産学部, 教授 (10032103)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSAWA Shuuichi Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (80130037)
MATSUDA Youichi Hokkaido University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (70165835)
MATSUDA Haruo Hiroshima University, Faculty of Applied Biological Science, Professor, 生物生産学部, 教授 (80116863)
TAKAHASHI Hideaki National Institute of Agrobiological Resources, Senior Researcher, 遺伝資源第1, 研究官
TSUDZUKI Masaoki Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (70212058)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥31,660,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2001: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2000: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1999: ¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1998: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | chicken / QTL / DNA marker / linkage analysis / chromosome map / reference family / QTR |
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| Research Abstract |
[Establishment of reference family for QTL analysis and characterize the economical traits of chicken lines] : 2,930 individuals of F2 generation were established from Oh-shamo and White Leghorn mating. As the economical traits, body weight, shank length, egg production, egg weight, meat color, crude fat, crude protein, amino acid, pH, and myoglobin were measured. [Development of DNA markers and its typing] : Eighty functional genes were mapped on chromosome map by using direct R-banding FISH method. No.1, 2, 4, and 5 of chicken chromosomes had high homology with human chromosomes. Chicken Z chromosome was found to be high homology with No.5 and 9 of human chromosomes. Out of 800 microsatellite DNA developed, 314 microsatellite DNA were informative in our reference family. [Analysis of immune-associated gene] : Recombinant protein from chicken IL-6 gene was identified and its activity was confirmed. An expression of mRNA of IL-6 gene was confirmed in GST cell line induced by IPTG stimulation.
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