| Budget Amount *help |
¥18,700,000 (Direct Cost: ¥18,700,000)
Fiscal Year 2000: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1998: ¥10,500,000 (Direct Cost: ¥10,500,000)
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| Research Abstract |
Through analyzing the alleles in Japanese and Chinese populations, we established a new nomenclature for the PCR-RFLP band patterns, closely relating them to their corresponding sequences so that the new types could be designated easily and accurately. The short fragments of 16 bp, 32 bp, 48 bp, 64 bp, and 80 bp contained 1,2,3,4, and 5 repeat units, respectively, and were thus named bands "1", "2", "3", "4", and "5". Excluding a flanking region, the eight kinds of long fragments of 221 bp, 237 bp, 253 bp, 269 bp, 285bp, 317 bp, 349bp, and 365 bp in length each contained 6,7,8,9,10,12,14, and 15 repeat units.
To testify whether alleles of the same length and of the same band pattern possessed the same sequences, a number of alleles were sequenced in addition to the above-sequenced samples. Of the sequenced samples which had the same lengths and the same band patterns, most shared the same sequences. However, some revealed different sequences. Because of the differences in the repeat arrays between the variants and the ladder markers, structural differences or one-sided destribution of the electrical charge led to more apparent migration in polyacrylamide gel electrophoresis.
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