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Studies of differentiation mechanisms of hematopoietic stem cells using cytokine-receptor transgenic mice

Research Project

Project/Area Number 10307020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKYOTO UNIVERSITY (1999)
The University of Tokyo (1998)

Principal Investigator

NAKAHATA Tatsutoshi  Graduate School of Medicine, KYOTO UNIVERSITY Professor, 医学研究科, 教授 (20110744)

Co-Investigator(Kenkyū-buntansha) YORIFUJI Tooru  Graduate School of Medicine, KYOTO UNIVERSITY Assistant Professor, 医学研究科, 助手 (60220779)
TSUJI Kohichiro  Institute of Medical Science University of Tokyo Associated. professor, 医科学研究所, 助教授 (50179991)
渡辺 すみ子  東京大学, 医科学研究所, 助手 (60240735)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥38,800,000 (Direct Cost: ¥38,800,000)
Fiscal Year 1999: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥33,700,000 (Direct Cost: ¥33,700,000)
Keywordshematopoietic stem cell / differentiation / G-CSF receptor / GM-CSF receptor / transgenic mice / EPOR - / - mice / stochastic model / clonal cell culture / サイトカイン / レセプター / トランスジェニック / マウス / 造血前駆細胞 / サイトカインレセプター / in vivo / in vitro / トランスジェニック(Tg)マウス
Research Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) stimulates proliferation and maturation of myeloid progenitor cells through cell surface receptors expressed on target cells. To analyze the relationship between receptor expression and differentiation of hemopoietic cells, transgenic mice (Tg mice) which express hGMR at all stages of hemopoitic cell development were generated using hGMR α and β cDNAs. hGM-CSF supported colony formation of various types including blast cell, erythroid (E), megakaryocyte (Meg), and mixed hematopoietic colonies. Administration of hGM-CSF to Tg mice resulted in increase of not only neutrophils, monocyte, eosinophils, NK cells but also erythrocytes in their peripheral blood. To clalify whether GM-CSFR signaling can compensate erythropoietin receptor (EPOR) signaling in erythropoiesis, we next generated double mutant mice, hGM-CSFR+/+ and EPOR-/-. Although double mutant mice were embryonic lethal, hGM-CSF induced a large number of erythroid colonies from day 14 fetal liver cells of double mutant mice. We also generated Tg mice that had ubiquitous expressions of wild type human G-CSFR, wild type murine G-CSFR. In methylcellulose colony assay of bone marrow and spleen cells G-CSF have an effect on promoting the proliferation and differentiation of not only granulocyte but also macrophage, megakaryocyte, mast cells, erythroid and more primitive hematopoietic progenitors in both serum-containing and -free culture. These results shows that when functional GM-CSFR or G-CSFR are present on the cell surface, both cytokines does not induce exclusive commitment to the neutrophil and macrophage lineages. In vitro and in vivo studies using the Tg mice indicated that cytokines such as hGM-CSF or hG-CSF supported the growth of various hematopoietic progenitors when the receptor was expressed, but did not alter their commitment program, thus favoring the "stochastic model" rather than the "deterministic model".

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (91 results)

All Other

All Publications (91 results)

  • [Publications] Sui X., Tsuji K., Ebihara Y.,TKishimoto T., Nakahata T.: "Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34+ cells through gp130 signaling"Blood. 93. 2525-2532 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hasegawa s., Pawankar R., Suzuki K., Nakahata T, Furukawa S, Okumura K, Ra C: "Functional expression of the high affinity receptor for IgE (Fc epsilon RI) in human Platelets and its intracellular expression in human megakaryocytes"Blood. 93. 2543-2551 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Handa H., Suzuki T., Inaba T., Tsuji K.Nakahata T, Yazaki Y., Look T., Hirai H.: "Expression of E2a-HLF chineric protein induced T-cell apotosis, B-cell maturation arrest, and development of acute lymphoblastic leukemia"Blood. 93. 2780-2790 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kampuraj D., Saito H., Kaneko A., Fukagawa K., Tsuji K., Nakahata T.: "Characterization of mast-cell-committed progenitors present in human cord blood"Blood. 93. 3338-3346 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi M., Ueda K., Kojima S., Shimobo T., Nishihira H., Nakahata T.: "Serum granulocyte colony-stimulating factor levels in patients with chronic neutropenia of childhood : Modulation of G-CSF levels by myeloid precursor cell mass"Brit. J. Haematol.. 105. 486-490 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taniguchi T, Endo H, Asano S. Fujita T., Nakahata T, Motokura T.: "Expression of p21Cip1/Wsf1/Sdi1 and p27 Kip1 cyclin-dependent kinase inhibitors during human hematopoiesis"Blood. 83. 4167-4178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kinoshita T., Sekiguchi T., Xu M-J., Ito Y.,Tsuji K., Nakahata T., Miyajima A.: "Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis"Proc, Natl. Acad. Sci. USA. 96. 7265-7270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yang FC., Tsuji K., Oda A., Xu MJ, Ebihara Y, Watanabe S, Ikeda Y., Nakahata T: "Effects of human granulocyte colony-stimulating factor (hG-CSF) on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice"Blood. 94. 950-958 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T., Seki Y., Kubo M., Nakahata T, Yamada S. Yoshimura A.: "Suppression of STAT5 functions in liver, mammary grands, and T cells in cytokine-inducible SH2 protein-1(CISI) transgenic mice"Mol. Cell. Biol. 19. 6396-6407 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ui M., Takeda M., Arai T., Yamada K., Nakahata T., Tokino T, Nakamura Y, Iba H: "Retrovirus vectors designed for efficient transduction of cytotoxic or cytostatic genes"Gene Therapy. 6. 1670-1678 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii T., Nakahata T., Maekawa T.: "Expression of SDF-1/PBSF receptor CXCR4 on CD34+ human bone marrow cellsis a phenotypic alteration for committed lymphoid progenitors"J. Immunol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kajiwara M., Nonoyama S., Eguchi M., Imai K., Ochs HD., Nakahata T., Yata J.: "In vitro megakaryopocyte differentiation from CD34 positive cells is defective in patients with Wiscott-Aldrich syndrome and X-linked thrombocytopenia"Brit. J. Haematol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi M., Nakamura T., Sawada T., Nakahata T., Yokota T. Heike T.:: "Chimeric GM-CSF-leukemia inhibitory factor (LIF) receptor can transduce expansion signals in primitive hematopoietic progenitors lacking IL-6Ra, IL-11Ra and gp130"Mol. Cell. Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishiguro A., Nakahata T., Kato T., Suauki Y., Shimobo T.: "Age-related changes in serum thrombopoietin in children : high levels in infants by one month of age"Prediatr, Res.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Futaki M., Yamashita T., Yabe M., Kato S., Asano S., Nakahata T.:: "The IVS+4A-T mutation of the Fanconi Anemia gene FANCC is not associated with severe phenotype in Japanese patients"Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuoka S., Tsuji K., Xu M., Yang FC., Kaneko K., Kikuchi A., Nakahata T.: "CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages"Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ueda T., Yoshino H., Ebihara Y., Ito M., Tsuji K., Nakahata T:: "Ex vivo expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin interleukin-6 receptor"J. Clin. Invest.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayashi Y., Honma Y., Niitsu N., Taki T., Tsuji K., Nakahata T.: "SN-1, a novel leukemic cell line with t(11;16)(q23;p13) : Myeloid characteristics and resistance to resistance to retinoids and vitamin D3"Cancer Res.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugiyama H., Nonaka T., Kishimoto T., Komoriya K., Tsuji K., Nakahata T.: "Peroxisome proliferator-activated receplors negatively regurate mast cell activation"FEBSLetters. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugiyama H., Nonaka T., Kishimoto T., Komoriya K., Tsuji K., Nakahata T.: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells : a possible role for these receptors in negative regulation of mast cell activation"Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sui X., Tsuji K., Ebihara Y.: "Tanaka R., Yasukawa K., Taga T., Kishimoto T., Nakahata T. Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34+ cells through gp130 signaling."Blood. 93. 2525-2532 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hasegawa S., Pawankar R., Suzuki K., Nakahata T., Furukawa S., Okumura K., Ra C.: "Functional expression of the high affinity receptor for lgE (Fc epsilon RI) in human platelets and its intracellular expression in human megakaryocytes."Blood. 93. 2543-2551 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Honda H., Suzuki T., Inaba T., Oda H., Tsuji K. Ebihara Y., Ishikawa T., Nakahata T., Yazaki Y., Look T., Hirai H.: "Expression of E2a-HLF chineric protein induced T-cell apotosis, B-cell maturation arrest, and development of acule lymphoblastic leukemia."Blood. 93. 2780-2790 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kampuraj D., Saito H., Kaneko A., Fukagawa K., Nakayama M., Tomikawa M., Tachimoto H., Ebisawa M., Akasawa A., Miyagi T., Kimura H., Nakajima T., Tsuji K., Nakahata T.: "Characterization of mast-cell-committed progenitors present in human cord blood."Blood. 93. 3338-3346 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inamitsu T., Nakahata T., Ueda K. : Juvenile chronic myelogenous leukemia: "Retrospective analysis in Japan and literature review."Int. J. Pediatr. Hematology/Oncology. 6. 123-129 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi M., Ueda K., Kojima S., Ishiguro A., Shimbo T., Nishihira H., Nakahata T.: "Serum granulocyte colony-stimulating factor levels in patients with chronic neutropenia of childhood : Modulation of G-CSF levels by myeloid precursor cell mass."Brit. J. Haematol.. 105. 486-490 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Taniguchi T., Endo H., Chikatsu N., Uchimaru K., Asano S., Fujita T., Nakahata T., Motokura T.: "Expression of p21Cip1/Waf1/Sdil and p27 Kipl cyclin-dependent kinase inhibitors during human hematopoiesis."Blood. 83. 4167-4178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kinoshita T., Sekiguchi T., Xu M-J., Ito Y., Kamiya A., Tsuji K., Nakahata T., Miyajima A: "Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis."Proc, Natl. Acad. Sci. USA. 96. 7265-7270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yang FC., Tsuji K., Oda A., Xu MJ., Ebihara Y., Kaneko A., Hanada S., Mitsui T., Kikuchi A., Manabe A., Watanabe S., lkeda Y., Nakahata T.: "Effects of human granulocyte colony-stimulating factor (hG-CSF) on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice."Blood. 94. 950-958 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto T., Seki Y., Kubo M., OhtsukalS., SuzukilA., Hayashi I., Tsuji K., Nakahata T., Okabe M., Yamada S., Yoshimura A.: "Suppression of STAT5 functions in liver, mammary grands, and T cells in cytokine-inducible SH2 protein-1 (CISI) transgenic mice."Mol. Cell. Biol.. 19. 6396-6407 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ui M., Takeda M., Arai T., Matsumoto K., Yamada K., Nakahata T., Furukawa Y., Tokino T., Nakamura Y., Iba H.: "Retrovirus vectors designed for efficient transduction of cytotoxic or cytostatic genes."Gene Therapy. 6. 1670-1678 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishii T., Nakahata T., Maekawa T.: "Expression of SDF-1/PBSF receptor CXCR4 on CD34+ human bone marrow cellsis a phenotypic alteration for committed lymphoid progenitors."J. Immunol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kajiwara M., Nonoyama S., Eguchi M., Hashimito H., Ochs HD., Nakahata T., Yata J.: "In vitro megakaryopocyte differentiation from CD34 positive cells is defective in patients with Wiscott-Aldrich syndrome and X-linked thrombocytopenia."Brit. J. Haematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi M., Nakamura T., Sawada T., Nakahata T., Yokota T., Heike T.: "Chimeric GM-CSF-leukemia inhibitory factor (LIF) receptor can transduce expansion signals in primitive hematopoietic progenitors lacking IL-6Ra, IL-11Ra and gp130."Mol. Cell. Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishiguro A., Nakahata T., Matsubara K., Hayashi Y., Kato T., Suzuki Y., Shimbo T.: "Age-related changes in serum thrombopoietin in children : high levels in infants by one month of age."Pediatr, Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Futaki M., Yamashita T., Yabe M., Kato S., Nakahata T.: "The IVS4+4A-T mutation of the Fanconi Anemia gene FANCC is not associated with severe phenotype in Japanese patients."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuoka S., Tsuji K., Xu M., Kikuchi A., Nakahata T.: "CD 34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ueda T., Yoshino H., Ebihara Y., Ito M., Tsuji K., Nakahata T.: "Ex vivo expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin interleukin-6 and soluble interleukin-6 receptor."J. Clin. Invest. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayashi Y., Honma Y., Tsuji K., Nakahata T.: "SN-1, a novel leukemic cell line with t (11;16) (q23;p13) : Myeloid characteristics and resistance to retinoids and vitamin D3."Cancer Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugiyama H., Nonaka T., Tsuji K., Nakahata T.: "Peroxisome proliferator-activated receptors negatively regurate mast cell activation."FEBS Letters. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugiyama H., Nonaka T., Tsuji K., Nakahata T.: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells : a possible role for these receptors in negative regulation of mast cell activation."Blood. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sui X.,Tsuji K.,Ebihara Y.,Tkishimoto T.,Nakahata T.: "Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34_+ cells through gp130 signaling"Blood. 93. 2525-2532 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hasegawa S.,Pawankar R.,Suzaki K.,Nakahata T.,Furukawa S.,Okumura K.,Ra C.: "Functional expression of the high affinity receptor for IgE (Fc epsilon RI) in human Platelets and its intracellular expression in human megakaryocytes"Blood. 93. 2543-2551 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Honda H.,Suzuki T.,Inaba T.,Tsuji K.Nakahata T.,Yazaki Y.,Look T.,Hirai H.: "Expression of E2a-HLF chineric protein induced T-cell apotosis, B-cell maturation arrest, and development of acute lymphoblastic leukemia"Blood. 93. 2780-2790 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kampuraj D.,Saito H.,Kaneko A.,Fukagawa K.,Tsuji K.,Nakahata T.: "Characterization of mast-cell-committed progenitors present in human cord blood"Blood. 93. 3338-3346 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kobayashi M.,Ueda K.,Kojima S.,Shimbo T.,Nishihira H.,Nakahata T.: "Serum granulocyte colony-stimulating factor levels in patients with chronic neutropenia of childhood: Modulation of G-CSF levels by myeloid precursor cell mass"Brit. J. Haematol.. 105. 486-490 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taniguchi T.,Endo H.,Asano S.,Fujita T.,Nakahata T.,Motokura T.: "Expression of p21Cip1/Waf1/Sdil and p27 Kip1 cyclin-dependent kinase inhibitors during human hematopoiesis"Blood. 83. 4167-4178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kinoshita T.,Sekiguchi T.,Xu M-J.,Ito Y.,Tsuji K.,Nakahata T.,Miyajima A.: "Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis"Proc, Natl. Acad. Sci. USA. 96. 7265-7270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yang FC.,Tsuji K.,Oda A.,Xu MJ.,Ebihara Y.,Watanabe S.,Ikeda Y.,Nakahata T.: "Effects of human granuloyle colony-stimulating factor (hG-CSF) on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice"Blood. 94. 950-958 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto T.,Seki Y.,Kubo M.,Nakahata T.,Yamada S.,Yoshimura A.: "Suppression of STAT5 functions in liver,mammary grands, and T cells in cytokine-inducible SH2 protein-1 (CIS1) transgenic mice"Mol. Cell. Biol.. 19. 6396-6407 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ui M.,Takeda M.,Arai T.,Yamada K.,Nakahata T.,Tokino T.,Nakamura Y.,Iba H.: "Retrovirus vectors designed for efficient transduction of cytotoxic or cytostatic genes"Gene Therapy. 6. 1670-1678 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishii T.,Nakahata T.,Maekawa T.: "Expression of SDF-1/PBSF receptor CXCR4 on CD34+ human bone marrow cells a phenotypic alteration for committed lymphoid progenitors"J. Immunol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Kajiwara M.,Nonoyama S.,Eguchi M.,Imai K.,Ochs HD.,Nakahata T.,Yata J.: "In vitro megakaryopocyt differentiation from CD34 positive cells is defective in patients with Wiscott-Aldrich syndrome and X-linked thrombocytopenia"Brit. J. Haematol.. (in pres).

    • Related Report
      1999 Annual Research Report
  • [Publications] Takagi M.,Nakamura T.,Sawada T.,Nakahata T.,Yokota T.,Heike T.: "Chimeric GM-CSF-leukemia inhibitory factor (LIF) receptor can transduce expansion signals in primitive hematopoietic progenitors lacking IL-6Ra, IL-11Ra and gp130"Mol.Cell.Biol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishiguro A.,Nakahata T.,Kato T.,Suzuki Y.,Shimbo T.: "Age-related changes in serum thrombopoietin in children:high levels in infants by one month of age"Pediatr, Res.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Futaki M.,Yamashita T.,Yabe M.,Kato S.,Asano S.,Nakahata T.: "The IVS4+4A-T mutation of the Fanconi Anemia gene FANCC is associated with severe phenotype in Japanese patients"Blood. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsuoka S.,Tsuji K.,Xu M.,Yang FC.,Kaneko K.,Kikuchi A.,Nakahata T.: "CD 34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages"Blood. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueda T.,Yoshino H.,Ebihara Y.,Ito M.,Tsuji K.,Nakahata T: "Ex vivo expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin interleukin-6 and soluble interleukin-6 receptor"J. Clin. Invest.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi Y.,Honma Y.,Niitsu N.,Taki T.,Tsuji K.,Nakahata T.: "SN-1, a novel leukemic cell line with t(11;16) (q23;p13): Myeloid characteristics and resistance to retinoids and viamin D3"Cancer Res.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiyama H.,Nonaka T.,Kishimoto T.,Komoriya K.,Tsuji K.,Nakahata T.: "Peroxisome prolifertor-activated receptors negatively regurate mast cell activation"FEBS Letters. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiyama H.,Nonaka T.,Kishimoto T.,Komoriya K.,Tsuji K.,Nakahata T.: "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells:a possible role for these receptors in negative regulation of mast cell activation"Blood. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 中畑龍俊: "In vitro での末梢血幹細胞の増幅.症例に学ぶ G-CSF の臨床"医薬ジャーナル. 14 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中畑龍俊、二木真琴: "遺伝子治療開発研究ハンドブック"日本遺伝子治療学会編、エヌ・テイ・エス. 6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 辻浩一郎、中畑龍俊: "内科学教科書 (印刷中)"文光堂(東京).

    • Related Report
      1999 Annual Research Report
  • [Publications] Manabe A., Mori T., Ebihara Y., Nakahata T., et al: "Characterization of leukemic cells in CD2/CD19 double positive acute lymphoblastic leukemia." Int.J.Hematol.67. 45-52 (1998)

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      1998 Annual Research Report
  • [Publications] Mukouyama Y., Hara T., Xu M., Nakahata T., et al: "Induction of hematopoiesis by oncostatin M in the AGM region." Immunity. 8. 105-114 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toru H., Eguchi M., Yata J., Nakahata T., et al: "IL-4 promotes the development of tryptase and chymase double positive human mast cells accompanied with cell maturation." Blood. 91. 187-195 (1998)

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      1998 Annual Research Report
  • [Publications] Nakahata T.: "Characteristics of hemopoietic stem/progenitor cells in bone marrow,peripheral blood and cord blood." Int.J.Pediatr.Hematol.Oncology. 5. 60-61 (1998)

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      1998 Annual Research Report
  • [Publications] Takahashi T., Yamada K., Nakahata T., Hirai H., et al: "A novel molecular approach to ex vivo hematopoietic expansion with recombinant EGFR-expressing adenovirus vector." Blood. 91. 4509-4515 (1998)

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      1998 Annual Research Report
  • [Publications] Nishihara M., Wada Y., Nakahata T., Maekawa T., et al: "A combination of stem cell factor and granulocyte colony-stimulating factor enhances the growth of human progenitor B cells supported by murine stromal cell line MS-5." Eur.J.Immunol.28. 855-864 (1998)

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      1998 Annual Research Report
  • [Publications] Tsuji K., Muraoka K., Nakahata T., et al: "Interferon-g and human megakaryopoiesis." Leukemia and Lymphoma. 31. 107-113 (1998)

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      1998 Annual Research Report
  • [Publications] Tajika K., Ikebuchi K., Nakahata T., Asano S., et al: "IL-6 and SCF exart different effects on megakaryocyte maturation." Brit.J.Haematol.100. 105-111 (1998)

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      1998 Annual Research Report
  • [Publications] Ishiguro A., Ishikita T., Naritake S., Nakahata T., et al: "Elevation of serum thrombopoietin precedes thrombocytopoiesis in Kawasaki disease." Thromb.Haemostasis. 79. 1096-1100 (1998)

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      1998 Annual Research Report
  • [Publications] Yoshida K., Taga T., Nakahata T., Kishimoto T., et al: "Myocardial,hematological, and placental disorders caused by targeted disruption of gp130, acommon signal transducer for IL-6 family of cytokines." Contemporaty Immunology. 259-289 (1998)

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      1998 Annual Research Report
  • [Publications] Suzuki H., Takei M., Nakahata T., Fukamachi H., et al: "Inhibitory effect of adenosine on degranulation of human cultured mast cells upon cross-linking of FceRI." Biochem.Biophy.Res.Comm.242. 697-702 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato T., Watanabe S., Tsuji K., Nakahata T., et al: "Induction of the erythropoietin receptor gene and acquisition of responsiveness to erythropoietin by stem cell factor in HML/SE, a human leukemic cell line." J.Biol.Chem.273. 16921-16926 (1998)

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      1998 Annual Research Report
  • [Publications] Maruyama K., Tsuji K., Kodera Y., Nakahata T., et al: "Characterization of peripheral blood progenitor cells mobilized by Nartograstim (N-terminal replaced granulocyte colony-stimulating factor) in normal volunteers." Bone Marrow Transplant.22. 313-320 (1998)

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      1998 Annual Research Report
  • [Publications] Xu M., Tsuji K., Miyajima A., Nakahata T., et al: "Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cells." Blood. 192. 2032-2040 (1998)

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      1998 Annual Research Report
  • [Publications] Shichijo M., Nakahata T., Saito H.,Nagai H., et al: "The effect of anti-asthma drage on mediator release from cultured human mast cells." Clin.Exp.Allergy. 28. 1229-1236 (1998)

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      1998 Annual Research Report
  • [Publications] Sako M., Nakahaat T., Kishitomo T., Sugiyama H., et al: "Abnormal expression of Wilms'tumor gene WT1 in juvenile chronic myeloid leukemia and infantile monosomy 7 syndrome." Leukemia Res.22. 965-967 (1998)

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      1998 Annual Research Report
  • [Publications] Toru H., Pawankar R., Yata J., Nakahata T., et al: "Human mast cells produce interleukin-13 by high affinity IgE receptor cross-linking : enhanced IL-13 production by IL-4 primed human mast cells." J.Allergy and Clin.Immunol.(in press.). (1998)

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      1998 Annual Research Report
  • [Publications] Yang FC, Watanabe S., Ebihara Y., Nakahata T., et al: "Human G-CSF stimulates the development of primitive multipotential progenitors of human G-CSF receptor-transgenic mice,but does not affect their commitment in vitro and in vivo." Blood. 92. 1632-1640 (1998)

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      1998 Annual Research Report
  • [Publications] Nakahata T., Sui X., Kishimoto T., et al: "Ex vivo expansion of human primitive hemopoietic progenitors." Stem Cells.(in press.). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sui X., Tsuji K., Kishimoto T., Nakahata T., et al: "Soluble IL-6 receptor with IL-6 stimulates megakaryopoiesis from human CD34+cells through gp130 signaling." Blood. (in press.). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yang FC., Tsuji K., Ikeda Y., Nakahata T., et al: "Effects of human granulocyte colony-stimulating factor (hG-CSF) on megakaryopoiesis and platelet function in hG-CSF receptor-transgenic mice." Blood. (in press.). (1998)

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      1998 Annual Research Report
  • [Publications] Taniguchi T., Endo H., Nakahata T., Motokura T., et al: "Expression of p21Cip1/Waf1/Sdi1 and p27 Kip1 cyclin-dependent kinase inhibitors during human hematopoiesis." Blood. (in press.). (1998)

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      1998 Annual Research Report
  • [Publications] Duraisamy K., Saito H., Tsuji K., Nakahata T., et al: "Characterization of mast-cell-committed progenitors present in human cord blood." Blood. (in press.). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakahata T., Toru H., et al: "Development of human mast cells." Adv.Immunol.(in press.). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hasegawa S., Nakahata T., Okumura K., RaC., et al: "Functional expression of the high affinity receptor for IgE (Fc epsilon RI) in human platelets and its intracellular expression in human megakaryocytes." Blood. (in press.). (1998)

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      1998 Annual Research Report
  • [Publications] Hibino H., Tani K., Nakahata T., Asano S., et al: "Common Mamoset as a target preclinical primate for cytokine and gene therapy studies." Blood. (in press.). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi M., Ueda K., Nishihira H., Nakahata T., et al: "Serum granulocyte colony-stimulating factor levels in patients with chronic neutropenia of childhood : Modulation of G-CSF levels by myeloid precursor cell mass." Brit.J.Haematol.(in press.). (1998)

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      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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