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Induction of transplantation tolerance based on bio-molecular technique

Research Project

Project/Area Number 10307030
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionNational Children's Medical Research Center

Principal Investigator

SUZUKI Seiichi  National Children's Medical Research Center, Department of Experimental Surgery & Bioengineering, Director, 小児医療研究センター・実験外科生体工学部, 部長 (00111386)

Co-Investigator(Kenkyū-buntansha) RII Kou  National Children's Medical Research Center, Department of Experimental Surgery & Bioengineering, Researcher, 小児医療研究センター・実験外科生体工学部, 研究員 (60321890)
KIMURA Hiromitsu  National Children's Medical Research Center, Department of Experimental Surgery & Bioengineering, Chief Researcher, 小児医療研究センター・実験外科生体工学部, 室長 (80115477)
ENOSAWA Shin  National Children's Medical Research Center, Department of Experimental Surgery & Bioengineering, Chief Researcher, 小児医療研究センター・実験外科生体工学部, 室長 (40232962)
NAKAJIMA Hiroo  Kyoto Prefectural University of Medicine, Second Department of Surgery, Assistant, 第2外科, 助手 (70275212)
SHINOMIYA Takahisa  National Children's Medical Research Center, Department of Experimental Surgery & Bioengineering, Division of Research Promotion, Chief Researcher, 小児医療研究センター・実験外科生体工学部, 室長 (30196414)
田村 明彦  国立小児病院, 小児医療研究センター・実験外科生体工学部, 研究員
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥33,200,000 (Direct Cost: ¥33,200,000)
Fiscal Year 2000: ¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1999: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1998: ¥16,800,000 (Direct Cost: ¥16,800,000)
KeywordsFasL / CrmA / CTLA4Ig / Cre / loxP / adenovirus vector / FTY720 / liver transplantation / heart transplantation / ラット / 共刺激分子 / 相乗的免疫抑制効果 / B7.2 / CD28 / 拒絶反応 / caspase / アポトーシス / Cre-loxP system / Fash / アデノウィルスベクター / HVJ-リポゾーム / cre I lox P / 遺伝子導入 / 免疫貴容
Research Abstract

An adenovirus vector AxCALNFasL was constructed in order to transduce a gene for Fas-Ligand(FasL), requiring cotransfection of Cre recombinase-expressing vector AxCANCre for its expression. When intravenously injected AxCALNFasL, rat liver expressed FasL by co-administration of AxCANCre in a dose-dependent manner. The FasL-expressed livers survived significantly in the allogeneic recipients. However, a high level of FasL-expression induced animal death due to fulminant hepatitis. Furthermore, we successfully generated AxCALNLCrmA, a recombinant adenovirus expressing CrmA-gene with a Cre-mediated switching cassette. When allografted, the CrmA-expressed livers were resistant to rejection response in the recipients, resulting in marked prolongation of recipient survival. The formation of active caspase-3 was markedly inhibited in the Crm A gene-transfected hepatocytes. In addition, CTLA4Ig, a soluble recombinant fusion protein that contains the extracellular domain of CTLA4 and Fc portion … More of IgG1, strongly adheres to the B7 to block CD28-mediated costimulatory signals and inhibits in vitro and in vivo immune responses. We intravenously injected the adenovirus vector containing CTLA4Ig cDNA(AxCAhCTLA4Ig)to the heartgrafted rats immediately after grafting. The serum level of CTLA4Ig reached to maximum at 51-93μg/ml 3 to 7 days after gene-transfection and declined after 14 days, although detectable levels were observed up to 49 days. The graft survival time was significantly prolonged in the gene-transfected recipient rats. Down-regulation of IL-2 and IFNγ mRNA and persistence of IL-4 and IL-10 transcripts were observed in the graft-infiltrating cells. Peri-operative administration of FTY720, a new immunosuppressant inducing lymphocyte apoptosis, in combination with the CTLA4Ig gene-transfection resulted in synergistic prolongation of graft survival, and 33% of recipients acquired transplantation tolerance. We detected CTLA4Ig protein in the sera 49 days after grafting ; its levels were always hiher in the combination group than in the AxCAhCTLA4Ig-alone group. Thus, FTY720 would be highly effective for enhancing the CTLA4Ig expression by adenovirus-mediated gene transfer. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (153 results)

All Other

All Publications (153 results)

  • [Publications] Torayuki Okuyama: "Fas-mediated apoptosis is involved in the elimination of genetransduced hepatocytes with E1/E3-deleted adenoviral vectors."J Gastroenterol Hepatol. 13. 113-118 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihiko Kageyama: "Apoptosis is involved in acute cardiac allograft rejection in rats."Ann Thor Surg. 65(6). 1604-1609 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Torayuki Okuyama: "Efficient Fas-ligand gene expression in rodent liver after intravenous injection of a recombinant adenovirus by the use of a Cre-mediated switching system."Gene Therapy. 5. 1047-1053 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Shimizu: "A novel immunosuppressant, FTY720, increases the efficiency of a superantigeninduced peripheral T cell deletion whilst inhibiting negative selection in the thymus."Immunology. 94(4). 503-512 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.-D.Wang: "Lymphotactin : a key regulator of lymphocyte trafficking during acute graft rejection."Immunology. 95(1). 56-61 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li: "Prolonged survival of rat liver allografts transfected with Fas-ligand expressing plasmid."Transplantation. 66(11). 1416-1423 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda S: "Differential activation of c-Jun NH2-terminal and p38 pathways during FTY720-induced apoptosis of T lymphocytes that is suppressed by the extracellular signal-regulated kinase pathway."J Immunol. 162(2). 3321-3326 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yusuke Kita: "Prolonged cardiac allograft survival in rats systemically injected adenoviral vectors containing CTLA4Ig-gene."Transplantation. 68(6). 758-766 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Fujino: "On/off switching Fas-ligand gene expression in liver by Cre/loxP adenovirus vector system."Transplant Proc. 31(1,2). 753-754 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] X.-K.Li: "Effect of hepatocyte growth factor on chronic rejection in rat renal allografts."Transplant Proc. 31(1,2). 854-855 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Yuzawa: "Rescue effect of FTY720 on acute renal rejection in dogs."Transplant Proc. 31(1,2). 872 (1999)

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      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Kimura: "Possible defect of SCID M Ф/DC in acquired tolerance induction following rat into SCID xenogeneic bone marrow cell chimeras."Transplant Proc. 31(1,2). 980 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yutaka Yata: "An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphoshate(CL2MDP)."Methods in Cell Science. 21. 19-24 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Jing-Ding Wang: "Early induction of apoptosis in androgen-independent prostate cancer cell line by FTY720 requires caspase-3 activation."Prostate. 40(1). 50-55 (1999)

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      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayoshi Tokiwa: "SV40 large T antigen immortalization of rat hepatic stellate-like cells."In Vitro Cell Dev Biol-Animal. 35. 246-247 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Takahashi: "Acute renal allograft rejection in the canine : Evaluation with serial duplex doppler ultrasonography."Transplant Proc. 31. 1731-1734 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Fujino: "Nitrosyl-hemoglobin detected by near infrared spectroscopy in rat liver allografts."Transplant Int. 12(5). 307-315 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Okuyama: "Controlled Fas ligand gene expression by Cre/loxP-mediated switching syzytem : high levels of FasL expression result in lethal hepatitis."Transplant Proc. 31(7). 2695-2696 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Tamura: "Combination effect of tacrolimus and FTY720 in liver transplantation in rats."Transplant Proc. 31(7). 2785-2786 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kita: "Prolonged graft survival induced by CTLA4Ig-gene transfection combined with FTY720 administration in rat heart grafting."Transplant Proc. 31(7). 2787-2788 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] X-K.Li: "Immunosuppressive effect of triptolide in vitro."Transplant Proc. 31(5). 2056-2057 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiko Tamura: "Immunosuppressive therapy using FTY720 combined with tacrolimus in rat liver transplantation."Surgery. 127(1). 47-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiko Tamura: "Immunosuppressive therapy using FTY720 combined with tacrolimus in rat liver transplantation."Surgery. 127(1). 45-54 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yukitoshi Nagahara: "Evidence that FTY720 induces T cell apoptosis in vivo."Immunopharmacology. 48(1). 75-85 (2000)

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      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masakatsu Yamashita: "TCR-induced calcineurin activation regulates Th2 cell development by modifying the IL-4 receptor signaling complex."J Exp Med. 191(11). 1869-1879 (2000)

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      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga: "Adenovirus-mediated gene therapy for mucopolysaccharidosis VII : Involvement of cross-correction in wide-spread distribution of the gene-products and long-term effects of CTLA-4Ig co-expression."Molecular Theray. 1(5). 406-413 (2000)

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      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Sakuragawa: "Human aminiotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver."J Hum Genet. 45. 171-176 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li: "Inhibition of Fas-mediated fulminant hepatitis in CrmA genetransfected mice."Biochem Biophys Res Commun. 273(1). 101-109 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Michitaka Ozaki: "Racl regulates stress-induced, redox-dependent heat shock factor activation."J Biol Chem. 275(45). 35377-35383 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga: "Phenotype Correction in Murine Mucopolysaccharidosis Type VII by Transplantation of Human Amniotic Epithelial Cells After Adenovirus-Mediated Gene Transfer."Cell Transplantation. 9(5). 687-692 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Terada: "Inducing Proliferation of Human Amniotic Epithelial(HAE) Cells for Cell Therapy."Cell Transplantation. 9(5). 701-704 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin Enosawa: "Long-Term Culture of Glutamine Synthetase-Transfected HepG2 Cells in Circulatory Flow Bioreactor for Development for a Bioartificial Liver."Cell Transplantation. 9(5). 711-715 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Haruhiko Ueda: "Effect of a novel immunosuppressant, FTY720, on allograft survival after renal transplant in rats."Eur Surg Res. 32. 279-283 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga: "Engraftment of genetically-engineered amniotic epithelial cells corrects lysosomal storage in multiple areas of the brain in mucopolysaccharidosis type VII mice."Molecular Therapy. 3. 139-148 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masayuki Fujino: "In vitro prevention of cell-mediated xenograft rejection via the Fas/FasL-pathway in CrmA-transduced porcine kidney cells."Xenotransplantation. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masanao Ohba: "The combined therapy of CTLA4Ig-gene transfection with FTY720 : FTY720 may enhance the effect of gene therapy."World J Surg. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li: "Fulminant Hepatitis by Fas-Ligand Expression in MRL-lpr/lpr Mice Grafted with Fas-Positive Livers and Wild-Type Mice with Fas-Mutant Livers."Transplantation. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li: "Induction of lymphocyte apoptosis in rat liver allograft with ongoing rejection by FTY720."Clin Exp Immunol. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tatsuya Nakajima: "Isolation of rat amniotic cells and their cytological examination with an aim of a gene carrier."Cell Transplantation. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin Enosawa: "Bioreactor with glutamine synthtase-transfected recombinant HepG2 cells exhibits the ammonia removal activity needless of adding cofactors and substrates."Cell Transplantation. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Suzuki, Toshihiro Kakefuda, Kenji Chiba, Yukio Hoshino, Takafumi Kawaguchi, Hirotoshi Kataoka, Fazlur Rahman.: "An immunosuppressive regimen using FTY720 combined with cyclosporin in canine kidney transplantation."Transplant Int. 11(2). 95-91 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Torayuki Okuyama, Xiao-Kang Li, Naoko Funeshima, Masayuki Fuijino, Kyoko Sasaki, Yusuke Kita, Motomichi kosuga, Masahiko Takahashi, Hidetsugu Saito, Seiichi Suzuki, and Masao Yamada.: "Fasmediated apoptosis is involved in the elimination of gene-transduced hepatocytes with E1/E3-deleted adenoviral vectors."J Gastroenterol Hepatol. 13('Supple). 113-118 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] X.-K.Li, Y.Kita, A.Tamura, S,Enosawa, H.Amemiya, S.Suzuki: "Activation of Fas and perforin pathways in rat liver allograft rejection."Transplant Proc. 30(1). 19-21 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihiko Kageyama, Xiao-Kang Li, Seiichi Suzuki, Hidetoshi Suzuki, Kazuya Suzuki, Yukio Harada.: "Apoptosis is involved in acute cardiac allograft rejection in rats."Ann Thor Surg. 65(6). 1604-1609 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Torayuki Okuyama, Masayuki Fujino, Xiao-Kang Li, Naoko Funeshima, Motomichi Kosuga, Seiichi Suzuki, Masao Yamada.: "Efficient Fas-ligand gene expression in rodent liver after intravenous injection of a recombinant adenovirus by the use of a Cre-mediated switching system."Gene Therapy. 5. 1047-1053 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] C.Shimizu, X.-K.Li, M.Kimura, K.Hashimoto, K.Sugaya, M.Kubo, S.Suzuki, T.Nakayama.: "A novel immunosuppressant, FTY720, increases the efficiency of a superantigen-induced peripheral T cell deletion whilst inhibiting negative selection in the thymus."Immunology. 94(4). 503-512 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.-D.Wang, N.Nonomura, S.Takahara, B.-S.Li, H.Azuma, N.Ichimaru, Y.Kokado, K.Matsumiya, T.Miki, S.Suzuki, A.Okuyama.: "Lymphotactin : a key regulator of lymphocyte trafficking during acute graft rejeciton."Immunology. 95(1). 56-61 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Li, X.K., Okuyama T, Tamura A, Fujino M.Funeshima N, Kaneda Y, Kita Y, Enosawa S, Amemiya H, Suzuki S.: "Prolonged survival of recipient rats with Fas-ligand-transfected liver allografts by using HVJ-liposome."Transplant Proc. 30. 943 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Yan H, Li X-K, Amemiya H, Suzuki S, Hiromitu K.: "Prevention of experimentally induced autoimmune type I diabetes in rats by the new immunosuppressive reagent FTY720."Transplant Proc. 30. 1044-1045 (1998)

    • Description
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    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yuzawa K, Otsuka M, Taniguchi H, Takada Y, Sakurayama N, Jinzenji Y, Suzuki S, Fukao K.: "An effect of FTY720 on acute rejection in canine renal transplantation."Transplant Proc. 30. 1046 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Kazui T, kawabe A, Li X-K, Amemiya H, Suzuki S.: "Beneficial immunosuppressive effect of FTY720 combined with intrathymic injection of splenic cells on rat pancreaticoduodenal allograft."Transplant Proc. 30. 1067-1068 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Yan H, Li X-K, Amemiya H, Suzuki S, Kimura H.: "Prevention of recurrence of autoimmune diabetes type I in rats following pancreaticoduodenal transplantation."Transplant Proc. 30. 1071-1072 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kita Y, Li X-K, Hayashi S, Funeshima N, Enosawa S, Tamura A, Suzuki K, Kazui T, Amemiya H, Suzuki S.: "Prolonged rat cardiac allograft survival using adenoviral vector containing the CTLA4Ig gene."Transplant Proc. 30. 1079-1080 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
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      2000 Final Research Report Summary
  • [Publications] Eiji Kobayashi, Shin Enosawa. Akio Fujimura, Naoshi Kamada, Seiichi Suzuki: "Timing of lymphocyte transfusion and surgical damage for the development of lethal graft-versus-host disease in the rat."Surgery Today. 28(10). 1036-1041 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li, Torayuki Okuyama, Akihiko Tamura, Shin Enosawa, Yasufumi Kaneda, Shiro Takahara, Naoko Funashima, Masao Yamada, Hiroshi Amemiya, Seiichi Suzuki.: "Prolonged survival of rat liver allografts transfected with Fas-ligand expressing plasmid."Transplantation. 66(11). 1416-1423 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin Enosawa, Seiichi Suzuki, Xiao-Kang Li, Torayuki Okuyama, Masayuki Fujino, Hiroshi Amemiya: "Higher efficiency of retrovirus transduction in the late stage of primary culture of hepatocytes from nontreated than from partially hepatectomized rat."Cell Transplant. 7(4). 413-416 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kameyama Y, Li X-K, Suzuki S, Suzuki H, Suzuki K, Kazui T, Harada Y.: "Apoptosis in rat cardiac allograft rejection."Transplant Proc. 30(7). 2943 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Han Y-S, Ueda M, Tanabe S, Nakatani T, Kishimoto T, Suzuki S, Amemiya H, Kimura J.: "Lipoprotein(a)deposition is associated with the development of transplant renal arteriosclerosis in humans."Transplant Proc. 30(7). 3017-3020 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Kazui T, Kawabe A, Li XK, Funeshima N, Amemiya H, Suzuki S.: "Immunosuppressive effect of FTY720 on rat pancreas allograft."Transplant Proc. 30(7). 3417-3418 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yan H, Suzuki K, Li X-K, Amemiya H, Suzuki S, Hiromitsu K.: "Immunosuppressive effect of FTY720 on autoimmune diabetes models."Transplant Proc. 30(7). 3436-3437 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Amemiya H, Sakamoto K, Hasegawa T, Enosawa S, Suzuki S: "Research on an international network system for organ transplantation."Transplant Proc. 30(7). 3612-3615 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Kazui T, Kawabe A, Yan H, Li X-K, Kitazawa Y, Amemiya H, Suzuki S, Kimura H.: "Origin, occurrence, and function of microchimeric cells : V.Quantitative aspects of microchimerism following pancreaticoduodenal transplantation model in rats."Transplant Proc. 30(7). 3848 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki K, Kazui T, Kawabe A, Yan H, Li X-K, Kitazawa Y, Amemiya H, Suzuki S, Kimura H.: "Clonal expansion of T lymphocyte lineage cells in microchimeric cells."Transplant Proc. 30(7). 3879-3880 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda S, Minowa A, Suzuki S, Koyasu S.: "Differential activation of c-Jun NH2-terminal and p38 pathways during FTY720-induced apoptosis of T lymphocytes that is suppressed by the extracellular signal-regulated kinase pathway."J Immunol. 162(2). 3321-3326 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yusuke Kita, Xiao-Kang Li, Masanao Ohba, Naoko Funeshima, Shin Enosawa, Akihiko Tamura, Kazuya Suzuki, Hiroshi Amemiya, Shuji Hayashi, Teruhisa Kazui, Seiichi Suzuki.: "Prolonged cardiac allograft survival in rats systemically injected adenoviral vectors containing CTLA4Ig-gene."Transplantation. 68(6). 758-766 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Fujino, X.-K.Li, T.Okuyama, N.Funeshima, A.Tamura, S.Enosawa, Y.Kita, T.Amano, M.Yamada, H.Amemiya, S.Suzuki: "On/off switching Fas-ligand gene expression in liver by Cre/loxP adenovirus vector system."Transplant Proc. 31(1,2). 753-754 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Azuma, S.Takahara, M.Kitamura, J.-D.Wang, A.Wega, M.H.Sayegh, S.Suzuki, K.Matsumoto, T.Nakamura, A.Okuyama, Y.Katsuoka.: "Effect of hepatocyte growth factor on chronic rejection in rat renal allografts."Transplant Proc. 31(1,2). 854-855 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Yuzawa, M.Otsuka, H.Taniguchi, Y.Takada, N.Sakurayama, Y.Jinzenji, S.Suzuki, K.Fukao: "Rescue effect of FTY720 on acute renal rejection in dogs."Transplant Proc. 31(1,2). 872 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Kimura, H.Yan, T.Miyagi, S.Suzuki, H.Amemiya, M.Moriyama, K.Tanaka.: "Possible defect of SCID MΦ/DC in acquired tolerance induction following rat into SCID xenogeneic bone marrow cell chimeras."Transplant Proc. 31(1,2). 980 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yutaka Yata, Shin Enosawa, Seiichi Suzuki, Xiao-Kang Li, Akihiko Tamura, Hiromitsu Kimura, Terumi Takahara, Akiharu Watanabe.: "An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphoshate(CL_2MDP)."Methods in Cell Science. 19-24 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Jing-Ding Wang, Shiro Takahara, Norio Nonomura, Naotsugu Ichimaru, Kiyohide Toki, Haruhito Azuma, Kiyomi Matsumiya, Akihiko Okuyama, Seiichi Suzuki.: "Early induction of apoptosis in androgen-independent prostate cancer cell line by FTY720 requires caspase-3 activation."Prostate. 40(1). 50-55 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayoshi Tokiwa, Yutaka Yata, Terumi Takahara, Akiharu Watanabe, Shin Enosawa, Seiichi Suzuki, Junko Kano, Masayuki Noguchi: "SV40 large T antigen immortalization of rat hepatic stellate-like cells."In Vitro Cell Dev Biol-Animal. 35. 246-247 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Takahashi, Y.Narumi, S.Takahara, S.Suzuki, M.Kyo, M.Cruz, M.Takamura, Y.Kokado, N.Ichimaru, K.Toki, H.Nakamura, A.Okuyama.: "Acute renal allograft rejection in the canine : Evaluation with serial duplex doppler ultrasonography."Transplant Proc. 31. 1731-1734 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiko Kakefuda, Shin Enosawa, Xia-Kang Li, Akihiko Tamura, Naoko Funeshima, Masaru Kanashiro, Hiroshi Amemiya, Masaki Kitajima, Seiichi Suzuki.: "Nitrosyl-hemoglobin detected by near infrared spectroscopy in rat liver allografts."Transplant Int. 12(5). 307-315 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Fujino, T.Okuyama, X-K.Li, N.Funeshima, A.Tamura, S.Enosawa, T.Amano, H.Amemiya, S.Suzuki: "Controlled Fas ligand gene expression by Cre/loxP-mediated switching syzytem : high levels of FasL expression result in lethal hepatitis."Transplant Proc. 31(7). 2695-2696 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Tamura, X-K.Li, N.Funeshima, S.Enosawa, H.Amemiya, S.Suzuki: "Combination effect of tacrolimus and FTY720 in liver transplantation in rats."Transplant Proc. 31(7). 2785-2786 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kita, X-K.Li, N.Funeshima, S.Enosawa, A.Tamura, S.Hayashi, K.Suzuki, T.Kazui, H.Amemiya, S.Suzuki: "Prolonged graft survival induced by CTLA4Ig-gene transfection combined with FTY720 administration in rat heart grafting"Transplant Proc. 31(7). 2787-2788 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Lu, M.Hachida, S.Enosawa, X.-K.Li, S.Suzuki, H.Koyanagi: "Immunosuppressive effect of triptolide in vitro."Transplant Proc. 31(5). 2056-2057 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiko Tamura, Xiao-Kang Li, Naoko Funeshima, Shin Enosawa, Hiroshi Amemiya, Masaki Kitajima, Seiichi Suzuki.: "Immunosuppressive therapy using FTY720 combined with tacrolimus in rat liver transplantation."Surgery. 127(1). 47-54 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yukitoshi Nagahara, Shin Enosawa, Masahiko Ikekita, Seiichi Suzuki, Takahisa Shinomiya: "Evidence that FTY720 induces T cell apoptosis in vivo."Immunopharmacology. 48(1). 75-85 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masakatsu Yamashita, Makoto Katsuyama, Makio Iwashima, Motoko Kimura, Chiori Shimizu, Tohru Kamata, Tahiro Shin, Nobuko Seki, Seiichi Suzuki, Masaru Taniguchi, Toshinori Nakayama.: "TCR-induced calcineurin activation regulates Th2 cell development by modifying the IL-4 receptor signaling complex."J Exp Med. 191(11). 1869-1879 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga, Satori Takahashi, Kyoko Sasaki, Xiao-Kang Li, Masayuki Fujino, Hirofumi Hamada, Seiichi Suzuki, Masao Yamada, Nobutake Matsuo, and Torayuki Okuyama.: "Adenovirus-mediated gene therapy for mucopolysaccharidosis VII : Involvement of cross-correction in wide-spread distribution of the gene-products and long-term effects of CTLA-4Ig co-expression"Molecular Therapy. 1(5). 406-413 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Fujino, X.-K.Li, N.Funeshima, H.Kimura, T.Amano, M.Hashimoto, K.Okabe, H.Yaginuma, K.Mikoshiba, T.Shinomiya, S.Enosawa, H.Amemiya, S.Suzuki.: "Pig kidney target cell PK15 apoptosis was inhibited by the cytokine response modifier A gene via suppression of caspase activity."Transplant Proc. 32(2). 291-292 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Norio Sakuragawa, Shin Enosawa, Takashi Ishii, Ramasamy Thangavel, Toshihiko Tashiro, Torayuki Okuyama, Seiichi Suzuki: "Human aminiotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver."J Hum Genet. 45. 171-176 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li, Masayuki Fujino, Lei Guo, Torayuki Okuyama, Naoko Funeshima, Mitsuhiro Hashimoto, Keiko Okabe, Hiroyuki Yaginuma, Katsuhiko Mikoshiba, Shin Enosawa, Hiroshi Amemiya, Seiichi Suzuki.: "Inhibition of Fas-mediated fulminant hepatitis in CrmA gene-transfected mice."Biochem Biophys Res Commun. 273(1). 101-109 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Michitaka Ozaki, Shailesh S.Deshpande, Piamsook Angkeow, Seiichi Suzuki, Kaikobad Irani.: "Rac1 regulates stress-induced, redox-dependent heat shock factor activation."J Biol Chem. 275(45). 35377-35383 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga, Shin Enosawa, Xiao-Kang Li, Seiichi Suzuki, Nobutake Matsuo, Masao Yamada, Jayanta Roy-Chowdhury, Osamu Koiwai, Torayuki Okuyama.: "Strong, Long-Term Transgene Expression in Rat Liver Using Chicken b-Actin Promoter Associated With Cytomegalovirus Immediate-Early Enhancer(CAG Promoter)."Cell Transplantation. 9(5). 675-680 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga, Satori Takahashi, Kyoko Sasaki, Shin Enosawa, Xiao-Kang Li, Sanae Okuyama, Masayuki Fujino, Seiichi Suzuki, Masao Yamada, Nobutake Matsuo, Norio Sakuragawa, Torayuki Okuyama.: "Phenotype Correction in Murine Mucopolysaccharidosis Type VII by Transplantation of Human Amniotic Epithelial Cells After Adenovirus-Mediated Gene Transfer."Cell Transplantation. 9(5). 687-692 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Terada, Keiko Matsuura, Shin Enosawa, Masao Miki, Akinori Hoshika, Seiichi Suzuki, Norio Sakuragawa: "Inducing Proliferation of Human Amniotic Epithelial(HAE)Cells for Cell Therapy."Cell Transplantation. 9(5). 701-704 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin Enosawa, Tomoyuki Miyashita, Seiichi Suzuki, Xiao-Kang Li, Miyuki Tsunoda, Hiroshi Amemiya, Mitsugu Yamanaka, Shinya Hiramatsu, Naoko Tanimura, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura.: "Long-Term Culture of Glutamine Synthetase-Transfected HepG2 Cells in Circulatory Flow Bioreactor for Development for a Bioartificial Liver."Cell Transplantation. 9(5). 711-715 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H, Takahara S, Azuma H, Kusaka M, Suzuki S, Katsuoka Y.: "Effect of a novel immunosuppressant, FTY720, on allograft survival after renal transplant in rats."Eur Surg Res. 32. 279-283 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] G.Lei, H.Amemiya, S.Suzuki, T.Goto, T.Kokubo, M.Miyamoto, H.Kimura: "New immunosuppressive reagent, FTY720, spares immunologic memory."Transplant Proc. 32(7). 1628 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Ohba, X.-K.Li, Y.Kita, A.Tamura, S.Enosawa, S.Sasakuri, S.Ogoshi, H.Amemiya, S.Suzuki.: "Perioperative administration of FTY720 and CTLA4IG in rat heart transplantation."Transplant Proc. 32(7). 2024-2025 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Kita, X.-K.Li, M.Ohba, N.Funeshima, S.Enosawa, H.Nogimura, S.Ohi, Y.Kageyama, K.Matsushita, Y.Ito, T.Takahashi, K.Suzuki, S.Suzuki, T.Kazusi.: "Adenoviral vectors containing the CTLA41G-gene inhibit chronic rejection in heterotopically transplanted rat tracheas."Transplant Proc. 32(7). 2036-2037 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Kimura, Y.Hua, T.Goto, T.Kokubo, H.Amemiya, S.Suzuki, M.Miyamoto, K.Tanaka: "Microchimerism and allograft acceptance : is it caused by microchimerism or graft antigens?"Transplant Proc. 32(7). 2045-2046 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Hua, T.Miyagi, H.Saito, D.P.Gold, X.-K.Li, M.Fujino, T.Taga, H.Amemiya, S.Suzuki, T.Kokubo, H.Kimura.: "Cytokine requirement for the development of rat dendritic cells by in vitro culturing of bone marrow cells."Transplant Proc. 32(7). 2078-2079 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Adachi, A.Tamura, A.Sugioka, M.Morita, H.Yan, X.-K.LI, Y.Kitazawa, H.Amemiya, S.Suzuki, M.Miyata, H.Kimura.: "Evidence of regulatory T lymphocytes that constitute peripheral blood microchimerism following rat liver Transplantation."Transplant Proc. 32(7). 2297-2299 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Miyashita, S.Enosawa, S.Szuki, A.Tamura, H.Tanaka, H.Amemiya, T.Matsumura, T.Omasa, K.Suga, T.Aoki, Y.Koyanagi: "Development of a bioartificial liver with glutamine synthetase-transduced recombinant human hepatoblastoma cell line, HepG2."Transplant Proc. 32(7). 2355-2358 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Hikino, T.Miyagi, Y.Hua, S.Hirohisa, D.P.Gold, X.-K.Li, M.Fujino, T.Tetsuya, H.Amemiya, S.Suzuki, L.Robb, M.Miyata, H.Kimura.: "GM-CSF-independent development of dendritic cells from bone marrow cells in the GM-CSF-receptor-deficient mouse."Transplant Proc. 32(7). 2458-2459 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Adachi, X.-K.Li, L.Guo, M.Fujino, N.Funeshima, H.Kimura, H.Amemiya, S.Suzuki: "High efficacy of gene transfer and expression using adenovirus vector in the rat liver transplantation model."Transplant Proc. 32(7). 2514-2515 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Masaki, K.Suzuki, H.Yan, X.-K.Li, Y.Kitazawa, H.Amemiya, S.Suzuki, H.Kimura.: "Evidence for radiosensitive regulatory T cells that constitute peripheral microchimerism after pancreaticoduodenal transplantation."Transplant Proc. 32(7). 2481-2482 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Masaki, K.Suzuki, H.Yan, X.-K.Li, Y.Kitazawa, H.Amemiya, S.Suzuki, T.Goto, H.Kimura.: "Quantitative aspects of microchimerism after rat small bowel and pancreaticoduodenal transplantation."Transplant Proc. 32(7). 2483-2484 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masayuki Fujino, Xiao-Kang Li, Takashi Suda, Mitsuhiro Hashimoto, Keiko Okabe, Hiroyuki Yaginuma, Katsuhiko Mikoshiba, Lei Guo, Torayuki Okuyama, Hiroshi Amemiya, Takashi Amano, Shin Enosawa, Seiichi Suzuki.: "In vitro prevention of cell-mediated xenograft rejection via the Fas/FasL-pathway in CrmA-transduced porcine kidney cells."Xenotransplantation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masanao Ohba, Xiao-Kang Li, Yusuke Kita, Shin Enosawa, Naoko Funeshima, Haruko Nagai, Hui-qi Zhang, Torayuki Okuyama, Shohei Ogoshi, Shiro Sasaguri, Hiroshi Amemiya, Seiichi Suzuki.: "The combined therapy of CTLA4Ig-gene transfection with FTY720 : FTY720 may enhance the effect of gene therapy."World J Surgery. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiao-Kang Li, Masayuki Fujino, Atsushi Sugioka, Miwa Morita, Torayuki Okuyama, Lei Guo, Naoko Funeshima, Hiromitsu Kimura, Shin Enosawa, Hiroshi Amemiya, Seiichi Suzuki.: "Fulminant Hepatitis by Fas-Ligand Expression in MRL-lpr/lpr Mice Grafted with Fas-Positive Livers and Wild-Type Mice with Fas-Mutant Livers."Transplantation. 71(4). 503-508 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] X.-K.Li, A.Tamura, M.Fujino, T.Kakefuda, N.Funeshima, S.Enosawa, M.Amari, S.Naoe, H.Amemiya, S.Suzuki.: "Induction of lymphocyte apoptosis in rat liver allograft with ongoing rejection by FTY720."Clin Exp Immunol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Motomichi Kosuga, Kyoko Sasaki, Xiao-Kang Li, Hidenori Ohkawa, Ikuko Ogino, Osamu Okuda, Hajime Arai, Norio Sakuragawa, Yuko Kamata, Noriyuki Azuma, Seiichi Suzuki, Masao Yamada, and Torayuki Okuyama: "Engraftment of genetically engineered amniotic epithelial cells corrects lysosomal storage in multiple areas of the brain in mucopolysaccharidosis type VII mice."Molecular Therapy. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hua Lu, Shin Enosawa, Kazuhiro Ohmi, Seiichi Suzuki.: "Proliferative response of P51 knock-out mouse-derived vascular smooth muscle cell line, P53LMAC01, to PDGF, compare with human aortic smooth muscle cells."Transplant Immunol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tatsuya Nakajima, Shin Enosawa, Tasuku Mitani, Xiao-Kang Li, Seiichi Suzuki, Hiroshi Amemiya, Osamu Koiwai, Norio Sakuragawa.: "Isolation of rat amniotic cells and their cytological examination with an aim of a gene carrier."Cell Transplantation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin Enosawa, Tomoyuki Miyashita, Yuji Fujita, Seiichi Suzuki, Hiroshi Amemiya, Takesho Omasa, Shinya Hiramatsu, Kenichi Suga, Toshiharu Matsumura.: "Bioreactor with glutamine synthtase-transfected recombinat HepG2 cells exhibits the ammonia removal activity needless of adding cofactors and substrates."Cell Transplantation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masayuki Fujino, Xiao-Kang Li, Yusuke Kitazawa, Naoko Funeshima, Lei Guo, Torayuki Okuyama, Takashi Amano, Hiroshi Amemiya, Seiichi Suzuki: "Selective repopulation of mice liver after fas-resistant hepatocytes transplantation."Cell Transplantation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiko Tamura: "Immunosuppressive therapy using FTY720 combined with tacrolimus in rat liver transplantation."Surgery. 127(1). 45-54 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yukitoshi Nagahara: "Evidence that FTY720 induces T cell apoptosis in vivo."Immunopharmacology. 48(1). 75-85 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masakatsu Yamashita: "TCR-induced calcineurin activation regulates Th2 cell development by modifying the IL-4 receptor signaling complex."J Exp Med. 191(11). 1869-1879 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Motomichi Kosuga: "Adenovirus-mediated gene therapy for mucopolysaccharidosis VII : Involvement of cross-correction in wide-spread distribution of the gene-products and long-term effects of CTLA-4Ig co-expression."Molecular Therapy:. 1(5). 406-413 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Norio Sakuragawa: "Human aminiotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver."J Hum Genet. 45. 171-176 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Xiao-Kang Li: "Inhibition of Fas-mediated fulminant hepatitis in CrmA gene-transfected mice."Biochem Biophys Res Commun. 273(1). 101-109 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Michitaka Ozaki: "Rac1 regulates stress-induced, redox-dependent heat shock factor activation.:"J Biol Chem. 275(45). 35377-35383 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Motomichi Kosuga: "Phenotype Correction in Murine Mucopolysaccharidosis Type VII by Transplantation of Human Amniotic Epithelial Cells After Adenovirus-Mediated Gene Transfer."Cell Transplantation:. 9(5). 687-692 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoshi Terada: "Inducing Proliferation of Human Amniotic Epithelial (HAE) Cells for Cell Therapy."Cell Transplantation. 9(5). 701-704 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shin Enosawa: "Long-Term Culture of Glutamine Synthetase-Transfected HepG2 Cells in Circulatory Flow Bioreactor for Development for a Bioartificial Liver."Cell Transplantation. 9(5). 711-715 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruhiko Ueda: "Effect of a novel immunosuppressant, FTY720, on allograft survival after renal transplant in rats."Eur Surg Res:. 32. 279-283 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Motomichi Kosuga: "Engraftment of genetically-engineered amniotic epithelial cells corrects lysosomal storage in multiple areas of the brain in mucopolysaccharidosis type VII mice."Molecular Therapy. 3. 139-148 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masayuki Fujino: "In vitro prevention of cell-mediated xenograft rejection via the Fas/FasL-pathway in CrmA-transduced porcine kidney cells."Xenotransplantation. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masanao Ohba: "The combined therapy of CTLA4Ig-gene transfection with FTY720: FTY720 may enhance the effect of gene therapy."World J Surg. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Xiao-Kang Li: "Fulminant Hepatitis by Fas-Ligand Expression in MRL-Ipr/lpr Mice Grafted with Fas-Positive Livers and Wild-Type Mice with Fas-Mutant Livers."Transplantation. (in press). (2000)

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      2000 Annual Research Report
  • [Publications] Xiao-Kang Li: "Induction of lymphocyte apoptosis in rat liver allograft with ongoing rejection by FTY720."Clin Exp Immunol. (in press). (2000)

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      2000 Annual Research Report
  • [Publications] Tatsuya Nakajima: "Isolation of rat amniotic cells and their cytological examination with an aim of a gene carrier."Cell Transplantation. (in press). (2000)

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      2000 Annual Research Report
  • [Publications] Shin Enosawa: "Bioreactor with glutamine synthtase-transfected recombinant HepG2 cells exhibits the ammonia removal activity needless of adding cofactors and substrates."Cell Transplantation. (in press). (2000)

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      2000 Annual Research Report
  • [Publications] Matsuda S., et al: "Differential activation of c-Jun NH2-terminal and p38 pathways during FTY720-induced apoptosis of T lymphocytes that is suppressed by the extracellular signal-regulated kinase pathway"J Immunol. 162(2). 3321-3326 (1999)

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      1999 Annual Research Report
  • [Publications] Yusuke Kita, et al: "Prolonged cardiac allograft survival in rats systemically injected adenoviral vectors containing CTLA4Ig-gene"Transplantation. 68(6). 758-766 (1999)

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      1999 Annual Research Report
  • [Publications] M. Fujino, et al: "On/off switching Fas-ligand gene expression in liver by Cre/lozP adenovirus vector system"Transplant Proc. 31(1, 2). 753-754 (1999)

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      1999 Annual Research Report
  • [Publications] X. -K. Li, et al: "Effect of hepatocyte growth factor on chronic rejection in rat renal allografts"Transplant Proc. 31(1, 2). 854-855 (1999)

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      1999 Annual Research Report
  • [Publications] K. Yuzawa, et al: "Rescue effect of FTY720 on acute renal rejection in dogs"Transplant Proc. 31(1, 2). 872 (1999)

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      1999 Annual Research Report
  • [Publications] H. Kimura, et al: "Possible defect of SCID M φ/DC in acquired tolerance induction following rat into SCID xenogeneic bone marrow cell chimeras"Transplant Proc. 31(1, 2). 980 (1999)

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  • [Publications] Yutaka Yata, et al: "An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphoshate (CL2MDP)"Methods in Cell Science. 21. 19-24 (1999)

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  • [Publications] Jing-Ding Wang, et al: "Early induction of apoptosis in androgen-independent prostate cancer cell line by FTY720 requires caspase-3 activation"Prostate. 40(1). 50-55 (1999)

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  • [Publications] Takayoshi Tokiwa, et al: "SV40 large T antigen immortalization of rat hepatic stellate-like cells"In Vitro Cell Dev Biol-Animal. 35. 246-247 (1999)

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  • [Publications] S. Takahashi, et al.: "Acute renal allograft rejection in the canine : Evaluation with serial duplex doppler ultrasonography"Transplant Proc.. 31. 1731-1734 (1999)

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      1999 Annual Research Report
  • [Publications] M. Fujino, et al: "Nitrosyl-hemoglobin detected by near infrared spectroscopy in rat liver allografts"Transplant Int. 12(5). 307-315 (1999)

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  • [Publications] T. Okuyama, et al: "Controlled Fas ligand gene expression by Cre/loxP-mediated switching syzytem : high levels of FasL expression result in lethal hepatitis"Transplant Proc. 31(7). 2695-2696 (1999)

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  • [Publications] A. Tamura, et al: "Combination effect of tacrolimus and FTY720 in liver transplantation in rats"Transplant Proc. 31(7). 2785-2786 (1999)

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      1999 Annual Research Report
  • [Publications] Y. Kita, et al: "Prolonged graft survival induced by CTLA4Ig-gene transfection combined with FTY720 administration in rat heart grafting"Transplant Proc. 31(7). 2787-2788 (1999)

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  • [Publications] X-K. Li, et al: "Immunosuppressive effect of triptolide in vitro"Transplant Proc. 31(5). 2056-2057 (1999)

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  • [Publications] Akihiko Tamura, et al: "Immunosuppressive therapy using FTY720 combined with tacrolimus in rat liver transplantation"Surgery. 127(1). 47-54 (1999)

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  • [Publications] Masanao Ohba, et al: "The combined therapy of CTLA4Ig-gene transfection with FTY720 : FTY720 may enhance the effect of gene therapy"World J Surg. (in press).

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  • [Publications] Yukitoshi Nagahara, et al: "Evidence that FTY720 induces T cell apoptosis in vivo"Immunopharmacology. (in press). (1999)

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  • [Publications] Torayuki Okuyama et al: "Fas-mediated apoptosis is involved in the elimination of gene-transduced hepatocytes with E1/E3-deleted adenoviral vectors." J Gastroenterol Hepatol. 13. 113-118 (1998)

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  • [Publications] Yoshihiko Kageyama et al: "Apoptosis is involved in acute cardiac allograft rejection in rats." Ann Thor Surg. 65・6. 1604-1609 (1998)

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      1998 Annual Research Report
  • [Publications] Torayuki Okuyama et al: "Efficient Fas-ligand gene expression in rodent liver after intravenous injection of a recombinant adenovirus by the use of a Cre-mediated switching system." Gene Therapy. 5. 1047-1053 (1998)

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      1998 Annual Research Report
  • [Publications] C.Shimizu et al: "A novel immunosuppressant,FTY720,increases the efficiency of a Superantigen-induced peripheral T cell deletion whilst inhibiting negative selection in the thymus." Immunology. 94・4. 503-512 (1998)

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      1998 Annual Research Report
  • [Publications] J.-D.Wang et al: "Lymphotactin: a key regulator of lymphocyte trafficking during acute graft rejection." Immunology. 95・1. 56-61 (1998)

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      1998 Annual Research Report
  • [Publications] Xiao-Kang Li et al: "Prolonged survival of rat liver allografts transfected with Fas-ligand expressing plasmid." Transplantation. 66・11. 1416-1423 (1998)

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      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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