| Project/Area Number |
10307041
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tohoku University |
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Principal Investigator |
TAMAI Makoto Tohoku Univ., Graduate School of med., Professor, 大学院・医学系研究科, 教授 (90004720)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Toshiaki Tohoku Univ., Graduate School of medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90191858)
NAKAGAWA Youichi Tohoku Univ., hospital, Lecture, 医学部附属病院, 講師 (10282063)
SATO Hajime Tohoku Univ., hospital, Research Assistant, 医学部附属病院, 助手 (10312571)
WADA Yuko Tohoku Univ., hospital, Research Assistant, 医学部附属病院, 助手 (70302130)
TOMITA Hiroshi Tohoku Univ., Graduate School of medicine, Research Assistant, 大学院・医学系研究科, 助手 (40302088)
小嶋 伸吾 東北大学, 医学部・附属病院, 助手
西川 真平 東北大学, 医学部・附属病院, 講師 (90261635)
武田 宜之 東北大学, 医学部・附属病院, 助手 (40292338)
布施 昇男 東北大学, 医学部・附属病院, 助手 (10302134)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥37,400,000 (Direct Cost: ¥37,400,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1998: ¥28,400,000 (Direct Cost: ¥28,400,000)
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| Keywords | IPE / age related macular degeneration / transplantation / 色素上皮細胞 / 加齢黄斑変性症 / 遺伝し導入 / サイトカイン / 遺伝子導入 |
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| Research Abstract |
Purpose. To establish autologous iris pigment epitheial cell transplantation in patients with age related macular degeneration. We investgated the survival of transplanted. IPE cells in monkey eye.
Methods. Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cells were performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by transvitreal approach to submacular region of each monkey. Fundus examination, photography, and fluorescein angiography were performed monthly. Histochemical analysis and electron microscopy were also performed six monthsafter transplantation Results. Auto IPE cells grew well in auto serum. No fluorescein leakage or retinal thickening was observed after submacular transplantation.
We demonstrated the presence of autologous IPE cells in the resion 6months after transplantation by histlogic and electron microscopy also demonstrated that the transplanted cells appeared to be less pigmented and to have less mitchondoria than did the host retinal pigment epithelial cells. However, some transplanted cells looked as if they embrace the photoreceptor outer segments.
Discussion. Autologous IPE cell culture of the monkey was established using auto serum. Six months after transplantaiton of of cultured IPE cells, we could still observe the cells in region. This method may have potential use in human desiese that reqires autologous cell transplantation to prevent host-graft rejection, and to provide an alternative substance that functions like retinal pigment epithelium
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