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Molecular biology of pathological conditions and diagnosis in dental pulp disorder and periodontal disease.

Research Project

Project/Area Number 10307047
Research Category

Grant-in-Aid for Scientific Research (A).

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTokushima University

Principal Investigator

NAGATA Toshihiko  Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Professor., 歯学部, 教授 (10127847)

Co-Investigator(Kenkyū-buntansha) KURIHARA Hidemi  Department of Periodontology and Endodontology, Hiroshima University Faculty of Dentistry, Professor., 歯学部, 教授 (40161765)
HASEGAWA Kohji  Department of Periodontics, Showa University Dental School, Professor., 歯学部, 教授 (70014024)
ISHIKAWA Isao  Periodontology, Faculty of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Professor., 大学院・医歯学総合研究科, 教授 (10014151)
MURAKAMI Shinya  Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Associate Professor., 大学院・歯学研究科, 助教授 (70239490)
MAITA Eikichi  Division of Periodontics and Endodontics, Tohoku University Graduate School of Dentistry, Associate Professor., 大学院・歯学研究科, 助教授 (80108547)
西村 英紀  岡山大学, 歯学部・附属病院, 講師 (80208222)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2000: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1999: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsdental pulp diseases / periodontal diseases / cytokine / gene / PCR / LPS / growth factors / fibroblasts / 転写因子 / 免疫組織化学 / アポトーシス / リポ多糖
Research Abstract

(1) Summary concerning dental pulp diseases : Type I collagen and osteopontin were found in human dental pulp stones and osteopontin expressin was stimulated by retinoic acid in culturted dental pulp cells. When the localization of DNA fragment after laser irradiation was investigated, dental pulps were affected at the early stage of cavity preparation. Expression of VEGF was induced by inflammatory cytokines in cultured dental pulp cells, which was mediated through AP-1 activations.
(2) Summary concerning periodontal diseases : RT-PCR analyses using inflammed gingival tissues of adult periodotitis revealed that high levels of P.gingivalis, T.denticola, and B.forsythus were found. Novel RT-PCR examination system using very little gingival tissues was established, in which over 20 mRNAs of inflammatory factors were detected from the periodontal lesions and semi-quantified the volume of the mRNAs. In cultured gingival fibroblasts, various proteins induced by IL-1 were transfered from predominant type to mature type. Adhesion molecules expressed by TNF-α were down-regulated by PGE_2 and I_2 in gingival epithelial cells. LPS form P.gingivalis inhibited osteoblastic differentiation of cultured rat calvaria cells. CD 14 was associated with calcium signal pathway stimulated by LPS.Expression of SPARC and OCIF was changed with the ageing of periodontal ligament cells and SPARC has an important role with the healing of periodontal tissues. In high glucose conditions, activity of cathepsin was inhibited in periodontal ligament cells. Phenytoin and Cyclosporin A were inhibited the expressions of MMP-1 and TIMP-1.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Ninomiya M,Nagata T, et al.: "Immunohistochemical localization of osteopontin in human pulp stones."Journal of Endodontics. 27(in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kobayashi H,Ishikawa I., et al.: "Individual diversities in interferron-δ production by human peripheral blood mono-nuclear cells stimulated with periodontpathic bacteria."Journal of Periodontal Research. 35. 319-328 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi Y,Hasegawa K, et al.: "Interferon-δ and interleukin 4 inhibit interleukin 1β-induced delayed prostaglandin E_2 generation through suppression of cyclooxygenase-2 expression."Cytokine. 12. 603-612 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba H,Kurihara H, et al.: "Effects of ageing on proliferative ability, and the expression of secreted protein, acidic and rich in cystein (SPARC) and osteoprotegerin(OIF)."Mech.Ageing Development. 117. 69-77 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura F,Murayama Y, et al.: "High glucose suppresses cathepsin activity in PDL cells."Journal of Dental Research. 79. 1614-1617 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsushita K,Torii M, et al.: "The role of vascular endotherial growth factor in human dental pulp cells."Journal of Dental Research. 79. 1596-1603 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 永田俊彦: "「腎疾患と歯周病」"先端医療シリーズ・歯科医学2「歯周病」""岡田宏,石川烈,村山洋二 監修,先端医療研究所,東京. 536 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 西村英紀: "「糖尿病と歯周病」"先端医療シリーズ・歯科医学2「歯周病」""岡田宏,石川烈,村山洋二 監修,先端医療研究所,東京. 536 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya M, Ohishi M, Kido J, Ohsaki Y, Nagata T.: "Immunohistochemical localization of osteopontin in human pulp stones."J Endodon. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kobayashi H, Nagasawa T, Aramaki M, Mahanonda R, Ishikawa I.: "Individual diversities in Interferron-gamma production by human peripheral blood mono-nuclear cells stimulated with periodontopathic bacteria."J Periodont Res. 35. 319-328 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi Y, Kobayashi M, Kuwata H, Atsumi G, Deguchi K, Xiu Feng Wei, Kudo I, Hasegawa K.: "Interferon-γ and Interleukin 4 inhibit Interleukin 1β-induced delayed prostaglandin E_2 generation through suppression of cyclooxygenase-2 expression in human fibroblasts."Cytokine. 12(6). 603-612 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba H, Nakanishi K, Sakata M, Fujita T, Uchida Y, Kurihara H.: "Effects of ageing on proliferative ability, and the expression of secreted protein, acidic and rich in cysteine (SPARC) and osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human peliodontal ligament cells."Mech Ageing Dev. 117. 69-77 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura F, Naruishi K, Yamada H, Kono T, Takashiba S, Murayama Y.: "High glucose suppresses cathepsin activity in PDL cells."J Dent Res. 79. 1614-1617 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsushita K, Motani R, Sakuta T, Yamaguchi N, Koga T, Matsuo K, Nagaoka S, Abeyama K, Maruyama I, Torii M.: "The role of vascular endorhelial growth factor in human dental pulp cells : Intduction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway."J Dent Res. 79(8). 1596-1603 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nagata T.: ""Renal Disorder and Periodontal Disease" in "SENTAN IRYOU SIRIESE SIKAIGAKU (2) SISHUBYOU""Okada H, Ishikawa I, Murayama Y KANSHUU, SENTAN-IRYOU-KENKYUSHO. 536 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishimura F.: ""Diabetes and Periodontal Disease" in "SENTAN IRYOU SIRIESE SIKAIGAKU (2) SISHUBYOU""Okada H, Ishikawa I, Murayama Y KANSHUU, SENTAN-IRYOU-KENKYUSHO. 536 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya.M,Nagata T, et al.: "Immunohistochemical localization of osteopontin in human pulp stones."Journal of Endodontics. 27(4)(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kobayashi H,Ishikawa I., et al.: "Individual diversities in interferon-σ production by human peripheral blood mono-nuclear cells stimulated with periodontpathic bacteria."Journal of Periodontal Research. 35(5). 319-328 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi Y,Hasegawa K, et al.: "Interferon-σ and interleukin 4 inhibit interleukin 1β-induced delayed prostaglandin E_2 generation through suppression of cyclooxygenase-2 expression."Cytokine . 12(6). 603-612 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shiba H,Kurihara H, et al.: "Effects of ageing on proliferative ability, and the expression of secreted protein, acidic and rich in cystein (SPARC) and osteoprotegerin (OIF)."Mech.Ageing Development . 117(1). 69-77 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishimura F,Murayama Y, et al.: "High glucose suppresses cathepsin activity in PDL cells."Journal of Dental Research. 79(8). 1614-1617 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsushita K,Torii M, et al.: "The role of vascular endotherial growth factor in human dental pulp cells."Journal of Dental Research. 79(8). 1596-1603 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 永田俊彦: "「腎疾患と歯周病」"先端医療シリーズ・歯科医学2「歯周病」""岡田宏,石川烈,村山洋二監修,先端医療研究所,東京. 536 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西村英紀: "「糖尿病と歯周病」"先端医療シリーズ・歯科医学2「歯周病」""岡田宏,石川烈,村山洋二監修,先端医療研究所,東京. 536 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagasawa T,Ishikawa I et al.: "The role of salivary IgA antibody against periodontopathic bacteria."Oral Diseases. Vol.5. 63-65 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagasawa T,Ishikawa I et al.: "Oral administration of Porphyromonas gingivalis fimbriae with cholera toxin induces anti-fimbriae serum IgG,IgM,IgA and salivary IgA antibodies."J Periodont Res. Vol.33. 169-174 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuragi K,Nishimura F et al.: "Immunopathological findings of an early-onset periodontitis patient with decreased CD 18 expression."J International Acad Periodontol. Vol.1(2). 35-44 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Asahara Y,Nishimura F et al.: "Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos."Oral Diseases. Vol.5(4). 337-343 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kobayashi M,Hasegawa K et al.: "Intracellular interleukun-1 α production in human gingival fibroblasts is differentially regulated by various cytokines."J Dent Res. Vol.78(4). 840-849 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsushita K,Motani R et al.: "Lipopolysaccharide enhances the production of Vascular Endothelial Growth Factor by Human pulp cells in culture."Infect Immun. Vol.67. 1633-1639 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi Y, Ishikawa I et al.: "Increased soluble CD14 serum levels in periodontitis patients." Infection and Immunity. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masaka T, Ishikawa I et al.: "Soluble CD14-dependent intracellular adhesion molecule 1 induction by P. gingivalis lipopolysaccharide in cultured human gingival fibroblasts." Journal of Periodontology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sawa T, Nishimura F et al.: "In vitro induction of activation induced cell death in lymphocytes from chronic lesion." Infection and Immunity. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshiba N, Yoshiba K et al.: "Immunohistochemical localization of class II antigens and nerve fibers in human carious teeth : HLA-DR immunoreactivity in Schwann cells." Archives of Histology and Cytology. 61. 343-352 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsushita K, Motani R et al.: "Lipopolysaccharide enhances the production of vascular endotherial growth factor by human pulp cells in culture." Infection and Immunity. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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