| Project/Area Number |
10307057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
HIGUCHI Hiroshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, PROFESSOR, 大学院・医歯学総合研究科, 教授 (30150337)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Masayoshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, ASSISTANT, 大学院・医歯学総合研究科, 助手 (40334677)
YAMAGUCHI Tsuyoshi GRADUATE SCHOOL OF MEDICAL AND DENTAL SCENCES. ASSISTANT, 大学院・医歯学総合研究科, 助手 (70323970)
MURAOKA Osamu GRADUATE SCHOOL OF MEDICAL AND DENTAL SCIENCES, LECTURER, 大学院・医歯学総合研究科, 講師 (20283765)
茂里 康 経済産業省, 大阪工業技術研究所・有機機能材料部, 主任研究員
吉田 豊 新潟大学, 医学部, 講師 (40182795)
仲澤 幹雄 新潟大学, 医学部, 助教授 (80143759)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥32,130,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2001: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2000: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1999: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1998: ¥16,600,000 (Direct Cost: ¥16,600,000)
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| Keywords | Neuropeptide Y / gene transcription / drug-induced regulation / Leptin / Zebrafish / transcriptional factor / NDF1 / JAK-STAT / Headgear / In situ hybridization / δEF1 / ZFH / NGF response element / repression / in situ hybridization |
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| Research Abstract |
Neuropeptide Y (NPY) is a cotransmitter of sympathetic neurons and is expressed abundantly in the central nervous systm. By using the rat NPY gene, the drug-induced regulatory mechanism of transcription of the neuronspecific genes has been studied and the drug-regulated transcriptional factors have been explored. Previously we have identified the unique enhancer elements, such as nerve growth factor (NGF) response element (NGFRE) and calcium/calmodulirrdependent kinase response element (CaRE). When studying these elements, we identified novel transcriptional factors, such as NDF1 and Kheper.
These transcriptional factors are involved in synaptic plasticity and neural differentiation. Many neuroactive drugs seem to affect the function of these transcriptional factors. In 2001, we investigated especially the following contents.
1. Leptin-induced transactivation of NPY gene promoter
2. Cloning of zebrafish NDF homolog
3. Change in gene Expression of NPY subtype in brain
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