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Determination of the entire structure of wheat chloroplast genome

Research Project

Project/Area Number 10309008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionFukui Prefectural University

Principal Investigator

TSUNEWAKI Koichiro  Fukui Prefectural University, President, 学長 (20026438)

Co-Investigator(Kenkyū-buntansha) TERACHI Toru  Kyoto Sangyo University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (90202192)
NAKAMURA Chiharu  Kyoto Sangyo University, Faculty of Agriculture, Professor, 農学部, 教授 (10144601)
OGIHARA Yasunari  Yokohama City University, Kihara Biological Research Institute, Associate Professor, 木原生物学研究所, 助教授 (40185533)
GOJOBORI Takashi  National Institute of Genetics, Center for Information Biology, Professor, 生命情報研究センター, 教授 (50162136)
MURAI koji  Fukui Prefectural University, Faculty of Biotechnology, Associate Professor, 生物資源学部, 助教授 (70261097)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥26,100,000 (Direct Cost: ¥26,100,000)
Fiscal Year 1999: ¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1998: ¥15,900,000 (Direct Cost: ¥15,900,000)
KeywordsCommon wheat / Chloroplast genome / Complete nucleotide sequence / Contig clones / Chloroplast genes / PCR primers
Research Abstract

Complete nucleotide sequence of chloroplast genome has been reported only in two Gramineae species, rice and corn. In this project, we succeeded determination of the complete sequence of wheat chloroplast genome as the third case. We used a common wheat (Triticum aestivum) cv. Chinese Spring as the material, because this cultivar has been used most extensively worldwide in genetic investigations. In addition, we constructed a contig clone bank of wheat chloroplast DNA. Also, PCR-primer sets were designed to amplify coding regions of all but two wheat chloroplast genes, using which their coding regions were successfully amplified. Main results are given below.
(1) Complete sequence of wheat chloroplast genome : Size of wheat chloroplast genome was 134,540 bp, that was 4.2% smaller than that of corn (140,387 bp) but almost the same as that of rice (134,525 bp). Sizes of three regions, large and small single copy regions (LSC and SSC) and inverted repeat (IR), were 80,347, 12,789 and 20,702 bp, respectively. This genome contained 109 genes, 4 (in duplication) coding for 4 rRNA species, 30 for tRNAs (corresponding to 20 amino acids), 70 for protein subunits and 5 of unidentified functions.
(2) Contig clone bank : A chloroplast DNA bank consisting of 55 contig clones (some are partially overlapping) that cover entire regions of LSC, SSC and IR was constructed. Average size of the clones was 2,246 bp.
(3) PCR primer sets for amplification of wheat chloroplast genes : A pair of PCR primers for each of 107 chloroplast genes were designed. Using these, small amounts of amplified DNAs were prepared for the coding regions of all but two wheat chloroplast genes.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ogihara, Y. 他20名: "Complete sequence of wheat chloroplast DNA"Proceedings of the 9th International Wheat Genetics Symposium. 3. 38-40 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, Y., Y. Matsuoka: "Chloroplast RNA polymerase subunit genes (rpo) in common wheat, Triticum aestivum cv. Chinese Spring"Proceedings of the 9th International Wheat Genetics Symposium. 3. 41-43 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, Y., H. Tajiri, Y. Matsuoka & K. Tsunewaki: "Molecular analysis of a 21.1-kb fragment of wheat chloroplast DNA bearing RNA polymerase subunit (rpo) genes"Genome. 42. 1042-1049 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ogihara, Y., K. Isono et al.: "Complete sequence of wheat chloroplast DNA"Proceedings of the 9th International Wheat Genetics Symposium. 3. 38-40 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, Y., Y. Matsuoka & K. Tsunewaki: "Chloroplast RNA polymerase subunit genes (rpo) in common wheat, Triticum aestivum cv. Chinese Spring"Proceedings of the 9th International Wheat Genetics Symposium. 3. 41-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi, Y., H. Tajiri, Y. Matsuoka & K. Tsunewaki: "Molecular analysis of a 21.1-kb fragment of wheat chloroplast DNA bearing RNA polymerase subunit (rpo) genes"Genome. 42.6. 1042-1049 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohnishi.Y., H.Tajiri, Y.Matsuoka & K.Tsunewaki: "Molecular analysis of a 21.1-kb fragment of wheat chloroplast DNA bearing RNA polymerase subunit (rpo) genes"Genome. 42・6. 1042-1049 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ogihara,Y.他20名: "Complete sequence of wheat chloroplast DNA" Proceedings of the 9th International Wheat Genetics Symposium. 3. 38-40 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohnishi,Y.,Y.Matsuoka and K.Tsunewaki: "Chloroplast DNA polymerase subunit genes(rpo)in common wheat,Triticum aestivum cv.Chinese Spring" Proceedings of the 9th International Wheat Genetics Symposium. 3. 41-43 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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