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Breeding of low prolamin rice

Research Project

Project/Area Number 10356001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Breeding science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SATOH Hikaru  Kyushu U., Faculty of Agriculture, Professor, 大学院・農学研究院, 教授 (70128031)

Co-Investigator(Kenkyū-buntansha) MOCHIZUKI Toshihiro  Kyushu U., Faculty of Agriculture, Assistant Professor, 大学院・農学研究院, 助手 (60239572)
KUMAMARU Toshihiro  Kyushu U., Faculty of Agriculture, Associate Professor, 大学院・農学研究院, 助教授 (00284555)
OGAWA Masahiro  Yamaguchi Pre. U., Faculty of Human life Science, Professor, 生活科学部, 教授 (10160772)
ARAMAKI Isao  National Research Institute of Brewing, Director, 酒類総合研究所, (研究職)室長
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥28,140,000 (Direct Cost: ¥27,000,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2001: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2000: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1999: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1998: ¥14,900,000 (Direct Cost: ¥14,900,000)
KeywordsRice / Brewing prosperity / prolamin / Linkage Analysis / Mutation / Protein Body / cDNA cloning / 胚乳貯蔵タンパク質 / 突然変異体 / cDNAライブラリー / SDS-PAGE / 遺伝分析 / MNU受精卵処理 / 旺気貯蔵タンパク質
Research Abstract

Screening of low prolamin mutants
Low prolamin mutant lines were selected from the progeny of the mutagenesis treatment. The diverse variation for prolamin polypeptides were detected from the native rice varieties, about 2000, collected from some countries. The crossing among low prolamin varieties was done, the progeny is being analyzed.
Brewing prosperity of low prolamin mutant
The low prolamin mutant, esp2, showed the low acid degree and low amino acid degree by small scale brewing test, though the mutant showed very high dissolving degree. The results will be helpful on the efficient utilization of the materials.
Isolation of the gene for low prolamin
By the results of the linkage analysis of the low prolamin mutant, esp1 gene is positioned within the 1.4 cM region on chromosome 4. The high-density linkage map of esp1 gene will be constructed.
The genes of PDI and BiP, related to the accumulation of the prolamin into PBI, were cloned using the cDNA library constructed from the developing seed of rice cultivar 'Kinmaze'. The specific antibodies were prepared based on the cDNA clone.
Cytochemical analysis of the low prolamin mutants
Prolamin consists of two classes, the cysteine residue poor prolamin (Cys-P prolamin) and the cysteine residue rich prolamin (Cys-R prolamin). The analysis of low prolamin mutants esp3 and Esp4 indicates that Cys-R and Cys-P prolamins are accumulated into PBI at the early and late stages, respectively, during seed development. The analysis by the antibodies to each prolamin classes indicates that Cys-P and Cys-R prolamins accumulate in the center and the periphery of PBI, respectively. It is thought that Cys-P prolamin plays an important role as a core and Cys-R plays the role of filling the inside of PBI.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Yoko Takemoto, Sean J.Coughlan, Thomas W.Okita, Hikaru Saioh, Masahiro Ogawa, Toshihiro Kumaimaru: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase"Plant Physiology. 128(印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jahan, M.S., T.Kumamaru, H.Satoh, A.Hamid: "Variation of glutelin seed storage protein in Bangladesh rice cultivars, a primary study"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aung, Pa Pa, T.Kumamaru, H.Satoh: "Genetic variation in seed storage protein glutelin of Myanmar local rice cultivars"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tian H.D., T.Kumamaru, H.Satoh: "Gene analysis of 57H mutant gene, glup6, in rice"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Satoh, Y.Uemura, T.Katayama, S.Minakuchi, T.Kumamaru, M.Ogawa: "Genetic diversity of endosperm storage nutrients in rice"Integration of Biodiversity and Genome Technology for Crop Improvemen. 89-92 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sadar Uddin Siddiqui, H.Satoh, T.Kumamaru: "Diversity in Pakistan rice genetic resources for endosperm storage"Integration of Biodiversity and Genome Technology for Crop Improvemen. 169-170 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Satoh, W.X.Li, Y.Takemoto, T.Ito, T.Kumamaru, L.Q.Qu, M.Ogawa: "glup4 controlling a 57H character was located on chromosome 12 in rice"Rice Genetics Newsletter. 16. 98-100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Takemoto, Sean J. Coughlan, Thomas W. Okita, Hikaru Satoh, Masahiro Ogawa, and Toshihiro Kumamaru.: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase"Plant Physiology. 128 (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jahan, M.S., T. Kumamaru, H. Satoh and A. Hamid: "Variation of glutelin seed storage protein in Bangladesh rice cultivars, a primary study"Rice Genetics Newsletters. 18 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aung, Pa Pa, T. Kumamaru, and H. Satoh: "Genetic variation in seed storage protein glutelin of Myanmar local rice cultivars"Rice Genetics Newsletter. 18 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tian H.D., T. Kumamaru, and H. Satoh: "Gene analysis of 57H mutant gene"glup6, in rice Rice Genetics Newsletter. 18 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Satoh, Y. Uemura, T. Katayama S. Minakuchi T. Kumamaru and M. Ogawa: "Genetic diversity of endosperm storage nutrients in rice"In 'Integration of Biodiversity and Genome Technology for Crop Improvement'. 89-92 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sadar Uddin Siddiqui, H. Satoh and T. Kumamaru: "Diversity in Pakistan rice genetic resources for endosperm storage"In 'Integration of Biodiversity and Genome Technology for Crop Improvement'. 169-170 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Satoh, W.X. Li, Y. Takemoto, T. Ito, T. Kumamaru, L.Q. Qu and M. Ogawa: "gluP4 controlling a 57H character was located on chromosome 12 in rice"Rice Genet. Newsl.. 16. 98-100 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Takemoto, Sean J.Coughlan, Thomas W.Okita, Hikaru Satoh, Masahiro Ogawa, Toshihiro Kumamaru: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase"Plant Physiology. 128(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jahan, M.S., T.Kumamaru, H.Satoh, A.Hamid: "Variation of glutelin seed storage protein in Bangladesh rice cultivars, a primary study"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aung, Pa Pa, T.Kumamaru, H.Satoh: "Genetic variation in seed storage protein glutelin of Myanmar local rice cultivars"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tian H.D., T.Kumamaru, H.Satoh: "Gene analysis of 57H mutant gene, glup6, in rice"Rice Genetics Newsletter. 18(印刷中). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Issiki,M.,M.Nakajima,H.Satoh and K.Shimamoto: "dull : rice mutants with tissue-specific effects on the splicing of the waxy pre-mRNA."The Plant Journal. 23(4). 451-460 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Matsusaka,T.Kumamar u,M.Ogawa and H.Satoh: "Biosynthesis of rice S-poor and S-rich prolamins are regulated by independent genetic system."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Takemoto,M.Ogawa,T.Kumamaru,T.W.Okita and H.Satoh: "Hypothetical model of genetic regulation of glutelin biosynthesis pathwey"Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.S.Jahan,T.Kumamaru,H.Satoh and H.Hamid: "Genetic diversity in seed storage proteins of Bangradesh rice cultivars"Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] P.P.Aung,T.Kumamaru and H.Satoh: "Genetic variation in seed storage protein and storage endosperm starch in Myanmar local rice cultivars."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.U.Siddiqui,H.Satoh and T.Kumamaru: "Variation in the seed storage protein in Pakistan rice germplasm."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Satoh,Y.Uemura,T.C.Katayama,S.Minakuchi,T.Kumamaru and M.Ogawa: "Integration of Biodiversity and Genome Technology for Crop Improvement"National Institute of Agrobiologicl Resources, Tukuba 305 8602, Japan. 181 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takahashi A.,T.Kawasaki,K.Henmi,K.Shii,O,Kodama,H,Satoh and K,Shimamoto: "Lesion-mimic mutants of rice with alterations in early signaling events of defense"The Plant Journal. 17(5). 535-545 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umemoto T.,Nakamura,H.Sat oh and K,Terashima: "Differ ence in amylopection struccture between tworice varieties in relation to the effects of temoerature during grain-filling"Starch. 51. 58-62 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hennmi K.,T,Kawasaki,S,Hatakeyama,M,Iwano,H,Satoh and K,Shimamoto: "The small GTP-binding protein Rac is a regulator of progammed cell death in plants"Proc,Natl,Acad,Sci. 96. 10992-10926 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kubo A.,Fujita,K.Harada,T,Matsuda,H,Satoh and Y,Nakamura: "Both types of starch debranching enzymes are involced in amulopection biosynthesis in rice endosperm"Plant Physiol,. 121. 399-409 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 谷 晃、林宏一郎、斉藤隆雄、佐藤 光、新井竜二、多胡靖宏、清田 信: "矮性イネから放出される微量ガス種の同定およびガス交換速度の測定"CELSS JOURNAL. 12(1). 15-20 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sat oh,H,W,X,Li,Y,Takemoto,T,Ito,T.Kumamaru,L,Q,Qu and M,Ogawa: "glup4 controlling a 57H char acter was located on choromosome 12 in rice,"RGN. 16(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takahashi A.,T.Kawasaki,K.Henmi,K.Shii,O.Komada,H.Satoh and K.Shimamoto: "Coordlnated acllvatlon of defenceresponses In lesion-mlmle mutants of rice" Plant. J.17(5)(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Umemoto T.,Y.Nakamura.H.Satoh andK.Terashima: "Difference in amylopectin structure belween two rice varleties In relatlon to the effects of temoerature during graln-filllng" Starch. 51(印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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