|Budget Amount *help
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥7,200,000 (Direct Cost: ¥7,200,000)
Models and the relevant equations giving spectroscopic data (optical absorbance, fluorescence intensity, or fluorescence lifetime) of an analyte (I.e., dye) confined in a small volume with different shapes and sizes (sample dimension; D) were given and, factors governing the data were discussed. The spectroscopic properties, particularly dye absorbance, were shown to be dependent on the three-dimensional sample structure: films (1-D), tubes (2-D), and spheres (3-D). The magnitude of the dimension effect on absorbance was determined by the size of both sample and probe beam. Furthermore, dye distribution characteristics in 2-D and 3-D systems also affected absorbance; a dye distributed exclusively to the surface layer of a 2-D or 3-D sample gave an absorbance 0.50 or 0.33-fold of that homogeneously distribution, respectively. If a dye distributed in the inner volume of the sample alone, absorbance increases over that for homogeneous distribution. Effects of sample dimension, dye distribution characteristics, and the size of the probe beam in absorption microspectroscopy were discussed on the basis of the proposed models. Similarly, the sample dimension as well as the dye distribution characteristics affected fluorescence intensity and fluorescence lifetime. For samples such as liquid and polymer films (1-D), microcapillary, microchannel chips, and liquid/liquid interface (2-D), and liquid droplets, polymer beads, microcapsules, giant vesicles (3-D), effects of sample dimension and dye distribution characteristics on the spectroscopic data were discussed in detail.