| Project/Area Number |
10440219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Nihon University |
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Principal Investigator |
SUGWARA Masao Nihon University, College of Humanities and Sciences, Professor, 文理学部, 教授 (50002176)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥8,900,000 (Direct Cost: ¥8,900,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥7,100,000 (Direct Cost: ¥7,100,000)
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| Keywords | Glutamate receptor channel / Agonist selectivity / NMDA / Bioseusor / Electroanalysis / Bilayer lipid membrane / Integrated current / liposome / イメージング / リポゾーム |
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| Research Abstract |
1. The magnitude of agonist-induced integrated single-channel current, corresponding to the number of ions passed through a single glutamate receptor (GluR) channel in bilayer lipid membranes (BLMs) during a unit time, was quanrified as a new measure of agonist selectivity. The agonist selectivity among the typical agonists, NMDA, L-glutamate and L-CCG-IC, in terms of the integrated single-channel current was in the order of L-CCG-IV>L-glutamate>NMDA.
2. To quantify the ion-permeation ability of the recombinant ε1-4/ζ1 N-methyl-D-aspartate (NMDA) channels activated by agonists, the magnitude of agonist-induced integrated single-channel currents for the ε1-4/ζ1 NMDA receptor channels in BLMs was evaluated. It was found that the magnitude of L-glutamate-induced integrated current depends on ε-subunit composition and the signal transduction abilty of the ε1-4/ζ1 NMDA receptor is not parallel to its binding ability.
3. The magnitude of Ca^<2+> release from GluR-incorporated liposomes, which was measured by a Ca^<2+> ion-selective electrode with a thin-layer mode, was proposed as a measure of agonist selectivity for NMDA receptor.
4. A single-channel method for evaluating agonist selectivity in terms of the very number of Ca^<2+> ions passed through the ε4/ζ1 NMDA receptor ion channel in BLMs was proposed.
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