| Project/Area Number |
10440249
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | Hiroshima University |
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Principal Investigator |
MATSUSHIMA Osamu Hiroshima University, Faculty of Science, Professor, 理学部, 教授 (80127711)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISAWA Yuko Suntory Institute for Bioorganic Research, Research Assistant, 生物有機科学研究所, 日本学術振興会特別研究員 (80274025)
MORISHITA Fumihiro Hiroshima University, Faculty of Science, Research Associate, 理学部, 助手 (20210164)
FURUKAWA Yasuo Hiroshima University, Faculty of Science, Associate Professor, 理学部, 助教授 (40209169)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Neuropeptides / Family peptides / Mollusca / Aplysia / Immunohistochemistry / Gut motility |
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| Research Abstract |
There seem to be many peptide families in invertebrates, each of which consists of multiple peptide species closely related in structure to each other. In this project, we have sought to understand the biological implication of such multiple family peptides in a given animal species and obtained the following results using one of the molluscan model animal, Aplysia. 1. A number of peptides were isolated from the central nervous system (CNS) of Aplysia kurodai, which showed inhibitory effect on gut motility and were classified into three groups according to their structures ; Aplysia inhlbitory peptides (AIP, or Enterins). Mytilus inhibitory peptides (MIP), and MIP-related pentapeptides (MIP-RP). 2. The cDNA for MIP was cloned, and the precursor protein contained 14 species of MIP. 3. Most, but not all, of those 14 peptides showed almost the same potency in a bioassay system. Furthermore, the order of the potency of bioactivity of the peptides was not always the same between assay systems, for example, gut and penis retractor muscle. 4. Northern analysis. whole-mount in situ hybridization, and immunohistochemistry showed the abundant expression of MIP in the CNS and peripheral tissues such as the digestive tract, vasculature, and the reproductive organs. The widespread distribution of MIP indicates that the peptides may be operating in many different systems. Although most of MIP appear to show functional redundancy, some peptides may have specialized functions.
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