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BASIC STUDY ON BIOPHOSPHORITE PRODUCTION BY ENHANCING BACTERIAL ABILITY TO SYNTHESIZE PHOSPHATE BIOPOLYMERS.

Research Project

Project/Area Number 10450312
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OHTAKE Hisao  FACULTY OF ENGINEERING, HIROSHIMA UNIVERSITY, PROFESSOR, 工学部, 教授 (10127483)

Co-Investigator(Kenkyū-buntansha) TAKIGUCHI Noboru  GRADUATE SCHOOL OF ADVANCED SCIENCES OF MATTER, HIROSHIMA UNIVERSITY, ASSISTANT, 大学院・先端物質科学研究科, 助手 (20304462)
IKEDA Tsukasa  FACULTY OF ENGINEERING, HIROSHIMA UNIVERSITY, ASSOCIATE PROFESSOR, 工学部, 助教授 (40151295)
KURODA Akio  GRADUATE SCHOOL OF ADVANCED SCIENCES OF MATTER, HIROSHIMA UNIVERSITY, ASSOCIATE PROFESSOR, 大学院・先端物質科学研究科, 助教授 (50205241)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordspolyphosphate / phosphorus recycling / biological phosphate removal / biophosphorite / ポリリン酸顆粒 / 生物脱リン / 富栄養化 / 資源問題 / 好気嫌気法 / バイオリン / 活性汚泥 / 細菌 / 遺伝子 / 環境バイオテクノロジー
Research Abstract

Phosphorus in wastewaters causes environmental pollution. On the other hand, phosphorus is essential in fertilizers for food production. However, natural phosphorus resources are being dissipated. The purpose of this research is to develop a bioprocess for recovering phosphorus from activated sludge, intending to prevent environmental pollution and to avert a crisis in phosphorus availability. In enhance biological phosphate removal, sludge bacteria accumulate large quantities of polyphosphate (polyP). The polyP could be released from activated sludge simply by heating at 70oC for one or two h. Released polyP could be easily precipitated by the addition of calcium chloride without pH control. The phosphorus content of the precipitates surpassed those of natural phosphorite deposits (NPD). Conversely, they had a relatively low calcium content, when compared with those of NPD.The bench-scale wastewater system, coupled with a process for phosphorus recovery, showed the high, stable performance for at least two months of operation. The present system will make a great contribution to both phosphorus recycling and eutrophication control.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Kuroda A.: "Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS."J.Biosc.Bioeng.. 90. 688-690 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda, A.: "Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS."J.Biosc.Bioeng.. 90. 688-690 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroda A.: "Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS."J.Biosc.Bioeng.. 90. 688-690 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akio Kuroda: "Molecular analysis ofpolyphosphateaccumulation in bacteria."Biochemistry(Moscow),(in press)..

    • Related Report
      1999 Annual Research Report
  • [Publications] 大竹久夫: "リン除去細菌の開発と活性汚泥からのポリリン酸熱回収技術"醸造協会誌. 95. 23-28 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 大竹久夫: "バイオレメディエーションを技術を活用する汚染環境の修復-リン資源の循環再利用のためのバイオテクノロジー"環境科学会誌. 12. 433-441 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akio Kuroda: "Inorganic polyphosphate kinase is requied to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli."Proc.Natl.Acad.Sci.USA.. 96. 14264-14269 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hong Wu: "Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system."J.Biosci.Bioeng.. 87. 273-279 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hisao Ohtake: "Environmental Monitoring and Biodiagnostics of Hazardous"Contaminants,Kluwer Academic Publishers(in press)..

    • Related Report
      1999 Annual Research Report
  • [Publications] Hisao Ohtake: "Progress in Molecular and Subcellar Biology"Springer-Verlag. 13 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuya Imazu: "Enhanced utilization of phosphonate and phosphite by kldosiella aerogenes" Applied and Environmental Microbiology. 64. 3754-3758 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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