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Molecular Mechanism of Target Recognition by Calmodulin

Research Project

Project/Area Number 10450358
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子構造・物性(含繊維)
Research InstitutionYamagata University

Principal Investigator

IZUMI Yoshinobu  Graduate School of Science and Engineering, Yamagata University Professor, 大学院・理工学研究科, 教授 (30002158)

Co-Investigator(Kenkyū-buntansha) JINBO Yuji  Graduate School of Science and Engineering, Yamagata University, Research Associate, 大学院・理工学研究科, 助手 (40311584)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsCalmodulin / Myosin light chain kinase / Calcineurin / Calmodulin-dependent protein kinase I, II, IV / CAP-23 / NAP-22 / W-7 / TFP / Recognition Sequence / 標準的標的分子 / 非標準的標的分子 / カルモデュリン依存性プロテインキナーゼ / アポカルモデュリン / NAPIZZ / 標的ペプチド / 分子認識モチーフ / 放射光小角散乱 / 多機能性発現機構 / 球状複合体 / 標的分子認識 / カルモデュリン依存性キナーゼI,II,IV / 1-5-8-14モチーフ / 1-5-10モチーフ
Research Abstract

9-1) Small-angle X-ray scattering (SAXS) was used to investigate the conformational changes of calmodulin on binding to a calmodulin-binding sequence in myosin light chain kinase, calcineurin, calmodulin-protein kinase. I, II, IV and these binding motifs were determined on the molecular level.
9-2) SAXS was used to investigate the conformational changes of calmodulin on binding to a myristoylated N-terminal nonapeptide from neuron-specific protein CAP-23/NAP-22 and the binding motif was determined on the molecular level.
9-3) SAXS was used to investigate the conformational changes of calmodulin on binding to an antagonist like W-7 and TFP and the binding motif was determined on the molecular level.
9-4) In the absence of Ca^<2+>, a new binding motif was found in the calmodulin/TFP and calmodulin/calmodulin-dependent protein kinase TV complexes.
9-5) SAXS was used to reveal the role of the central helix in calmodulin for its recognition.
9-6) SAXS with stopped-flow technique was used to reveal the kinetic conformational change of Ca^<2+>-dependent target recognition by calmodulin.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Y.Izumi, S.Saito,K.Soma: "Differential scanning calorimetry and structural studies of the sol-sel transition of gellan gum in water"Prog.Colloid.Polym.Sci.. 114. 48-55 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Osawa,S.Kumamoto,Y.Humi,K.L.Yap,M-lkura, 他: "Evidence for calmodulin inter-domain compaction in solution induced by W-7 binding"FEBS Letters. 442. 173-177 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Matsushima,N.hayachi,Y.Jinbo.Y-lzumi: "Ca^<2+>-bound calmodulin forms a compact globular structrue on binding four trifluoperazine molecules in solution"Biochem.J.. 347. 211-215 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Hayashi,Y.lzumi K.Titani,N.Matsushima: "The binding of myrystoylated N-terminal nonapeptide from neuron-specfic protein CAP-23/NAP-22 to calmodulin does not induce the globular structure"Protein Science. 9. 1905-1913 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.lzumi,H.Takezawa,N-Kikuta 他: "Two-stage Molting in Dilute Gels of poly (γ-benryl L-glutamate)"Macromolecules. 31. 430-435 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Kataoka,H.Kamikubo, T.Kanaya,Y.lzumi, 他: "Low energy dynamics of globular proteins studied by inelastic neutron scattering"J.Physics and chemistry of Solids. 60. 1285-1289 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Izumi, S.Saito, K.Soma: "Differential scanning calorimetry and structural studies of the sol-gel transition of gellan gum in water"Prog.Colloid.Polym.Sci.. 114. 48-55 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Osawa, S.Kuwamoto, Y.Izumi, K.L.Yap, M.Ikura, T.Shibayama, H.Yokokura, H.Hidaka, N.Matsushima: "Evidence for calmodulin inter-domain compaction in solution."FEBS Letters. 442. 173-177 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Matsushima, N.Hayasi, Y.Jinbo, Y.Izumi.: "Ca^<2+>-bound calmodulin forms a compact globular structure on binding four trifluoperazine molecules in solution"Biochem.J.. 347. 211-215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Hayashi, Y.Izumi, K.Titani, N.Matsushima: "The binding of myristoylated N-terminal nonapeptide from neuron-specific protein CAP-23/NAP-22 to calmodulin does not induce the olobular structure observed for the calmodulin-nonmyristoylated peptide complex"Protein Science. 9. 1905-1913 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Izumi, H.Takezawa, N.Kikuta, S.Uemura, A.Tsutsumi: "Two-Stage Melting in Dilute Gels of poly (g-benzyl L-glutamate)"Macromlecules. 31. 430-435 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Kataoka, H.Kamikubo, J.Yunoki, F.Tokunaga, T.Kanaya, Y.Izumi, K.Shibata: "Low energy dynamics of globular proteins studied by inelastic neutron scattering"J.Physics and Chemistry of Solids. 60. 1285-1289 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Matsushima, Y.Izumi, T.Aoba: "Small-Angle X-Ray Scattering and Computer-Aided Molecular Modeling Studies of 20kDa Fragment of Porcine Amelogenin : Does Amelogenin Adopt an Elongated Bundle Structure"J.Biochem.(Tokyo). 123. 150-156 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Matsushima,N.Hayashi,Y.Jinbo,Y.Izumi: "Ca^<2+>-bound calmodulin forms a compact globular structure on binding fourtrifluoperazine molecules in solution"Biochemical Journal. 347. 211-215 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Hayashi,Y.Izumi,K,Titani N.Matsushima: "The binding of myristoylated N-terminal nona peptide from neeuron-specific protein CAP-231 NAP-22 to calmodulin does not induce the globular structure observed for the calmodulin-nonmy-ristoykaled peptide complex"Protein Science. 9. 1905-1913 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 神保雄次,和泉義信: "超小角と小角散乱を用いた物理ゲルの構造研究"波紋. 10・2. 32-36 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] N.Sasaki, N.Shkunami, N.Matsushima, Y.Izumi: "Time-resolved X-ray diffraction from tender collagen during creep using synchrotron radiation"J. Biomechanics. 32. 285-292 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Kataoka, H.Kamikubo, J.Yunoki, F.Tokunaga, T.Kanaya, Y.Izumi, K.Shibata: "Low energy dynamics of globular, proteins studied by in elastic neutron scattering"J. Physicsα Chemistry of Solids. 60. 1285-1289 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Osawa, S.Kuwamoto, Y.Izumi, K.L.Yorp, M.Ikura, N.Matsusima: "Evidenae for calmodulin inter-dowain compaition in solution"FEBS Letters. 442. 173-177 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Sasaki, K.Tashiro, M.Kobayashi, Y.Izumi & K.Kobayashi: "Microspically viewed structural change of PE during the isothermal crystallization from the meet II"Polymer. 40・25. 7125-7135 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Izumi, S.Saito & K.Soma: "Differetial scanning calorimetry and structural studies of the sol-gel transition of gellan gam in water"Progr. Colloid. Polym. Sci.. 114. 48-55 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N.Matsushima, N.Hayashi, Y.Jinbo & Y.Izumi: "Calcium-bound calmodulin forms compact globular structure upon the binding of four trifluoperazine molecules in"J. Bio chemistry. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Osawa, S.Kuwamoto, Y.Izumi 他: "Evidence for calmodulin inter-domain compaction in solusion induced by W-7 binding" FEBs Lett.442. 173-177 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 和泉 義信: "アタクチックポリスチレン物理ゲルの構造と機構" 高分子論文集. 55.12. 749-759 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Izumi, S.Saito, K.Sowa: "DSC and structural studie ofset-gel transition of gellan sum in water" Prog.Coll.Polym.Sci.印刷中. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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