| Project/Area Number |
10450365
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YOSHIKAWA Kenichi Dept.of Phys., Kyoto University, Professor, 大学院・理学研究科, 教授 (80110823)
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| Co-Investigator(Kenkyū-buntansha) |
OANA Hidehiro Dept.of Phys., Assistant, 大学院・理学研究科, 助手 (20314172)
MURATA Shizuaki Graduate School of Human Informatics, Nagoya University, Professor, 大学院・人間情報学研究科, 教授 (50157781)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | Giant DNA / Folding Transition / Single Molecule Observation / Fluorescence Microscopy / DNA Condensation / Disorder-Order Transition / Phase Diagram of Single Chain / Laser Trapping / コイルーグロビュール転移 / 単分子観察 / 温度依存性 / 蛍光顕微鏡 / ポリアミン / 潜熱 / ボルツマン分布 |
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| Research Abstract |
We have performed a systematic study on the folding transition of single polymer chains both from experiment and theory.
(1) From numerical simulations of Monte Carlo and molecular dynamics, the phase-diagram of a single polymer has been deduced. In general, the transition is discerete for the change from elongated coil into order compact structure, i.e., discorder-order transition. Whereas, the transition is continuos from elongated coil into liquid-like globule state ; i.e., disorder-disorder transition.
(2) Experimental method to observe the structural transition on individual DNA chains has been established. It was found that, in the presence of polycation, DNA undergoes folding transition from elongated the compact state with increase of temperature. Contrary this, DNA chains exhibit unfolding transition in PEG-solution with increase of temperature.
(3) We have constructed an experimental system to trap individual DNA chains by IR laser. It was found that individual DNA molecules are transferred selectively as long as centimeter in aqueous solution. As an extension of such study, we have succeeded in observing rhythmic change between clongated and compact states in single DNA chains under suitable nonequilibrium condition.
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