| Project/Area Number |
10460026
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KAWAGUCHI Yutaka Faculty of Agriculture, Kyushu University, Associate Professor, 農学研究院, 助教授 (80038306)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Yutaka Faculty of Agriculture, Kyushu University, Associate Professor, 農学研究院, 助教授 (50192711)
FUJII Hiroshi Faculty of Agriculture, Kyushu University, Professor, 農学研究院, 教授 (10038268)
KOGA Katsumi Faculty of Agriculture, Kyushu University, Professor, 農学研究院, 教授 (40038261)
KUSAKABE Takahiro Faculty of Agriculture, Kyushu University, Asistant Professor, 農学研究院, 助手 (30253595)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | Bombyx mori / mutation / oogenesis / egg-shape trait / chorion trait / yolk protein / embryogenesis / gene expression / 致死遺伝子 |
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| Research Abstract |
1.Characteristics of the low temperature sensitive gray-egg mutation in Bombyx mori.
The low-temperature sensitive gray egg (tsg), which is a spontaneous mutation and is controlled by a single recessive gene belonging to the 5th linkage group, was characterized. This trait was expressed in a mosaic only when female pupae homozygous for tsg were subjected to a cold shock, which was most effectively conducted by chilling at 6 to 15℃ at a pupal age of day 5 or 6 after the larval-pupal ecdysis. Animals thus treated deposited a mixture of normal and gray eggs ; chilling for 10 hr at 10℃ produced about 8 gray eggs per individual. This number corresponds to the follicle population shortly before or after the onset of choriogenesis at a given moment, indicating that this phase may be critical for the cold response. Scanning electron microscopy indicated that the lamellar structure at the middle layer of the chorion region affected by the mutation was less uniform than normal.
2.Eggshell morpholo
… More
gy of the Eri-silkmoth, Samia cynthia ricini D.(Lepidoptera : Saturniidae).
The Samia cynthia ricini chorion was observed for its surface and inner structures by scanning electron microscopy. A micropyle opening was present at the anterior pole region of eggshell surface, surrounded by 12 to 20 petal-shaped cell imprints making a single rosette. There were 6 to 10 "sperm ducts, " dangling from the inside surface of chorion at the anterior pole region ; these were connected from the micropyle and probably supporting the polyspermy. All surface areas were confirmed to have network patterns made of comparatively uniform polygonal imprints. Large aeropyles opened at the junction of three polygonal units, and there was an additional single huge hole at the central region of each polygonal unit. Cross-sections of eggshell revealed the three layers, the outer, middle and inner. The middle layer, which was the thickest, was formed from three parts, upper, center and lower, into which the aeropyles and the additional holes penetrated. These layers were irregular lamellae, porous and parallel lamellae, and thus probably supporting the facileness of gas changes. Less
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