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Genome rearrangements of indigenous soybean bradyrhizobia and their ecological significance

Research Project

Project/Area Number 10460028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

MINAMISAWA Kiwamu  Graduate School of Life Sciences, Tohoku University, Professor, 大学院・生命科学研究科, 教授 (70167667)

Co-Investigator(Kenkyū-buntansha) TSUDA Masataka  Graduate School of Life Sciences, Tohoku University, Professor, 大学院・生命科学研究科, 教授 (90172022)
MITSUI Hisayuki  Graduate School of Life Sciences, Tohoku University, Associate Professor, 大学院・生命科学研究科, 助教授 (40261466)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsBradyrhizobium japonicum / genetic ecology / insertion sequence / nodulation / soil bacteria / soybean / stress resistance / 増殖 / ストレス / 土壌 / ダイズ根粒菌 / 遺伝的多様性 / 窒素固定 / Bradyrhizobium
Research Abstract

In order to understand the generation and ecological roles of Bradyrhizobium japonicum HRS strains possessing high copy numbers of insertion sequences (IS), we have investigated (1) geographical and phylogenetic distributions of HRS strains, (2) their excellent features in field ecosystem, (3) their genome rearrangements, and (4) horizontal transfer of nodulation (nod) gene from HRS strains. We found a correlation among the IS copy number, phylogenetic position, serogroup and growth rate of HRS strains, which are derived from USA, China and Japan. Nodule occupancy of HRS strains increased in a water-logged soil condition. Moreover, HRS strains showed stress resistance. The nif gene cluster of HRS strain were subjected to DNA rearrangement probably by IS elements. We have observed horizontal transfer of nod genes from B. japonicum HRS strain to B. elkanii USDA94 mutant lacking common nod genes as a recipient. In this project, we could show the ecological and genetic features of B. japonicum HRS strains.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Minamisawa, K.: "Horizontal transfer of nodulation genes in soil and microcosm from Bradyrhizobium japonicum to B. elkanii"Microbe Environ.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K.: "Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ"FEMS Microbiol. Ecol.. 29. 171-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Isawa, T.: "IS1631 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains with high copy numbers of repeated sequences RSα and RSβ"Appl. Environ. Microbiol.. 65. 3493-3501 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuda, M.: "Mobile catabolic gene in bacteria"J. Bio. Bioeng.. 87. 401-410 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K.: "New Bradyrhizobium japonicum that possess high copy numbers of the repeated sequence RSα"Appl. Environ. Microbiol.. 64. 1845-1851 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito, A.: "Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium japonicum"FEMS Microbiol. Eco.. 25. 277-286 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K.: "Chapter 8: Genetic ecology of soybean bradyrhizobia. In Soil Biochemistry Volume 10"Marcel Dekker, New York. 519(348-377) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K et al.: "Horizontal transfer of nodulation genes in soil and microcosm from Bradyrhizobium japonicum to B. elkanii"Microbe Environ.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K. et al.: "Genetic ecology of soybean bradyrhizobia"Marecel Dekker, Soil Biochemistry Vol. 10.,(2000), Edited by J. Bollag and G. Stotzky. pp. 348-377, New York.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K. et al.: "Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ"FEMS Microbiol. Ecol.. 29. 171-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Isawa, T. et al.: "IS1637 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains w|th high copy numbers of repeated sequences RSα and RSβ"Appl. Environ. Microbiol.. 65. 3493-3501 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuda, M. et al.: "Mobile catabolic gene in bacteria"J. Bio. Bioeng. 87. 401-410 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K. et al.: "New Bradyrhizobium japonicum that possess high copy numbers of the repeated sequence RSα"Appl. Environ. Microbiol.. 65. 1845-1851 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito, A. et al.: "Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium"FEMS Microbiol. Eco.. 25. 277-286 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minamisawa, K. et al.: "Horizontal transfer of nodulation genes in soil and microcosm from Bradyrhizobium japonicum to B. elkanii"Microbe Environ. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuta, T. et al.: "DNA sequence and mutational analysis of rhizobitoxine biosynthesis genes in Bradyrhizobium elkanii"Appl. Environ. Microbiol. 67. 4999-5009 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Elbeltagy, A. et al.: "Endophytic colonization and in planta nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species"Appl. Environ. Microbiol. 67. 5285-5293 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shigematsui, T. et al.: "Iron-mediated regulation of alkaline proteinase production in Pseudomonas aeruginosa"Microbiology and Immunology. 45. 579-590 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuda, M., Genka, H.: "Identification and characterization of Tn4656, a novel class II transposon carry a set of toluene-degrading genes From TOL plasmid pWW53"Journal of Bacteriology. 183. 6215-6224 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sawada, H. et al.: "Phylogenomic study of the OCTase genes in Pseudomnonas syringae pathovars : horizontal transfer of argK-tox cluster and the evolutionary history of OCTase genes on their genomes"Journal Molecular Evolution. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 南澤 究: "食糧生産・地球環境を支える植物の共生微生物、くらしの中の化学と生物8「人に役立つ微生物の話」"日本農芸化学会編、学会出版センター、東京(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono,Y. et al.: "Two RpoH homologs responsible for the expression of heat schock protein genes in Sinorhizobium meliloti"Mol.Gen.Genet.. 264. 902-912 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Junko K.Akada et al.: "Identification of the urease operon in Helicobacter pylori and its control by m-RNA decay in response to pH"Mol.Microbiol.. 36. 1071-1084 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Minamisawa et.al.: "Interspecies transfer of nodulation genes from B.japonicum HRS strains to B.elkanil"In Nitrogen Fixation:From Molecular to Crop Productivity. 291 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Minamisawa,K.and H.Mitsui: "Soil Biochemistry Vol.10(pp.349-377)"Marcel Dekker Inc.,New York. 519 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 南澤究: "地球の生命6,食う食われる生物たち。(pp.28-35)"三友社出版,東京. 191 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Minamisawa K.,Nakatsuka Y.,Isawa,T.: "Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ"FEMS Microbiol.Ecol.. 29. 171-178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Isawa T.,Sameshima R.,Mitsui H. And Minamisawa K.: "IS 1631 occurrence in Bradyrhizobium japonicum highly reiterated sequence-possessing strains with high copy numbers of repeated RSα and RSβ"Appl.Environ.Microbiol. 65. 3493-3501 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Minamisawa,K.et al.: "New Bradyrhizobium japonicum Strains That Possess High Copy Numbers of the Repeated Seguence RSα" Appl.Environ.Microbiol.65・5. 1845-1851 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saito,A.et al.: "Slow-growing and oligotrophic soil bacteria phylogenetically close to Bradyrhizobium japonicum" FEMS Microbiol.Ecol.25・3. 277-286 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuda,M.: "Use of a transposon-encoded site-specific resolution system for construction of large and defined deletions mutation in bacterial chromosome" Gene. 207・1. 33-41 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuta,T.,Satoh,S.and Minamisawa,K.: "New Assay for Rhizobitoxine Based on Inhibition of I-Aminocyclopropane-1-Carboxylate Synthase" Appl.Environ.Microbiol.65・2. 849-852 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minamisawa,K.et al: "Diversity and field site variation of indigenous populations of soybean brodyrhizobia in Japan by fingerprints with repeated seguences RSα and RSβ" FEMS Microbiol.Ecol.28. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minamisawa,K.and Mitsui,H.: "Genetic ecology of soybean bradyrhizobia.Soil Biochemistry vol.10" Marcel De kker.Inc., 600〜700 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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