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Intracellular dynamism of transcription factors and signal cross-talk in the stress response in yeast

Research Project

Project/Area Number 10460041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KIMURA Akira  Kyoto University, Research Institute for Food Science, Professor, 食糧科学研究所, 教授 (80026541)

Co-Investigator(Kenkyū-buntansha) IZAWA Shingo  Kyoto University, Research Institute for Food Science, Instructor, 食糧科学研究所, 助手 (10273517)
MANO Jun'ichi  Kyoto University, Research Institute for Food Science, Instructor, 食糧科学研究所, 助手 (50243100)
INOUE Yoshiharu  Kyoto University, Research Institute for Food Science, Associate Professor, 食糧科学研究所, 助教授 (70203263)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 1999: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1998: ¥9,100,000 (Direct Cost: ¥9,100,000)
KeywordsYap1 / glutathione peroxidase / thioredoxin / thioredoxin peroxidase / heat shock stress / グルタチオンペルオキシダーゼ / Zap1 / Msn2 / Osr1 / Saccharomyces cerevisiae
Research Abstract

a)Role of thioredoxin in regulation of Yap1 transcription factor in Saccharomyces cerevisiae.
Yap1 is an AP-1-like, b-Zip transcription factor in S. cerevisiae, and it regulates the expression of many genes encoding antioxidant enzymes. Yap1 is concentrated in the nucleus when the yeast cells are exposed to oxidative stress. We found that Yap1 is constitutively concentrated in the nucleus if thioredoxin, a thiol-disulfide oxidoreductase, was disrupted. Intracellular glutathione contents are increased if thioredoxin was destroyed (trx1△/trx2△), however, such a mutant showed increased sensitivity to oxidative stress even though intracellular glutathione levels are high. This was presumably due to the lack of thioredoxin.
b)Role of glutathione in heat shock stress response in yeast.
We found that oxygen respiration rate is increased if the yeast S. cerevisiae cells are shifted to higher temperature, and consequently intracellular oxidation level was increased. Additionally we found that expr … More ession of GSH1 and GSH2, both encoding glutathione-synthesizing enzymes, is increased by heat shock, and intracellular glutathione contents are also increased. In a gsh1 mutant, heat shock treatment induced the respiration deficiency at high rate. These results suggest that the increased production of glutathione under heat shock protects mitochondrial DNA from oxidative damage.
c)Genetic analysis of glutathione peroxidase in S. cerevisiae.
Three glutathione peroxidase (GPx) homologs were found in the yeast S.cerevisiae genome in the genome sequence database of this microorganism. We disrupted each of these homolog genes, and analyzed the function of each gene product. The GPX3 gene was found to be constitutively expressed, and the gpx3△ mutant was hypersensitive to oxidative stress. On the contrary, expression of the GPX2 gene was induced by oxidative stress in a Yap1-dependent fashion. Based on the analysis of the phenotype of a thioredoxin peroxidase mutant (tsal△), the GPX2 gene product was likely to function as a backup system for the thioredoxin peroxidase. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Inoue,Y. et al.: "Expression of the glyoxalase I gene of Saccharomyces cerevisiae is regulated by high osmolarity glycerol mitogen activated protein kinase pathway"Journal of Biological Chemistry. 273(5). 2977-2983 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Izawa,S., et al.: "Importance of glucose-6-phosphate dehydiogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae"Biochemical Journal. 330(2). 811-817 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue,Y. et al.: "Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae"Biochimica et Biophysica Acta. 1395. 315-320 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Asada,K., et al.: "Molecular mechanism for relaxation of and protection from light stress"Stress Response of Photosynthetic Organisms. 37-52 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 井上善晴: "酵母のストレス応答におけるグリオキサラーゼ遺伝子の発現制御"生化学. 70・10. 1278-1283 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue,Y., Kimura,A: "Oxidative stress response in Hansenula mrakii: a new type of glutathione peroxidase in yeast"Recent. Res. Dev. Agric. Biol. Chem.. 2. 29-39 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Izawa,S., et al.: "Thioredoxin-deficiency causes the constitutive activation of YAP-1, on AP-1 like transcription factor in Saccharomyces cerevisiae"J. Biol. Chem.. 274(40). 28459-28465 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue,Y. et al.: "Genetical analysis of the function of glutathione peroxidase in oxidative stress response of Sacchamyces cerevisiae"J. Biol. Chem.. 274(38). 27002-27009 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugiyama,K., Izawa,S., Inoue,Y.: "The Yapl-dependent induction of gluthione synthesis in heatshock response of Saccharomyces cerevisiae"J. Biol. Chem.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue,Y. et al.: "Glycoltic-methylglyoxal pathway, Molecular evolution and Stress response of glyoxalase I in Saccharomyces cerevisiae"Proc. Jpn. Acad.. 75B(6). 127-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 井上善晴: "Saccharomyces cerevisiaeのストレス応答におけるグルタチオン代謝の遺伝生化学的研究"日本農芸化学会誌. 73(10). 1013-1021 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 真野純一、浅田浩二: "光酸素ストレスを回避する分子機構"蛋白質核酸酵素. 44・15. 2239-2245 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 真野純一、木村光: "ラジカル測定による植物のストレス診断と応用"京都大学食糧科学研究所報告. 62. 26-27 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue, K. Sugiyama, S. Izawa and A. Kimura.: "Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae."Biochim. Biophys. Acta. 1395 (3). 315-320 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Izawa, K. Maeda, T. Miki, J. Mano, Y. Inoue and A. Kimura.: "Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae."Biochem. J.. 330 (2). 811-817 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue, Y. Tsujimoto and A. Kimura.: "Expression of the glyoxalase I gene of Saccharomyces cerevisiae is regulated by high osmolarity glycerol mitogen-activated protein kinase pathway in osmotic stress response."J. Biol. Chem.. 273 (5). 2977-2983 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Asada, T. Endo, J. Mano and C. Miyake.: "Molecular mechanism for relaxation of and protection from light stress."Stress Responses of Photosynthetic Organisms, (ed. by K. Satoh and N. Murata Elsevier). 37-52 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue.: "Regulation of the expression of glyoxalase I in the stress response in yeast."Seikagaku. 70 (10). 1278-1283 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue and A. Kimura.: "Oxidative stress response in Hansenula mrakii : a new type of glutathione peroxidase in yeast."Recent Research Developments in Agricultural and Biological Chemistry, (ed. by S. G. Pandalai Research Signpost, Trivandrum). Vol.2. 29-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Mano and A. Kimura.: "Diagnosis of plant stress by electron spin resonance spectrometry and its application."Bull. Res. Inst. Food Sci., Kyoto U. 62. 26-27 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Mano and K. Asada.: "Molecular mechanisms of the water-water cycle and other systems to circumvent photooxidative stress in plants."Tanpakushitu Kakusan Kouso. 44 (15). 2239-2245 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue.: "Genetical and biochemical studies on glutathione metabolism in stress response of Saccharomyces cerevisiae."Nihppon Nougei-kagaku Kaishi. 73 (10). 1013-1021 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue, T., Matsuda, K., Sugiyama, S. Izawa and A. Kimura.: "Genetic analysis of the function of glutathione peroxidase in oxidative response of Saccharomyces cerevisiae."J. Biol. Chem.. 274 (38). 27002-27009 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Izawa, K. Maeda, K. Sugiyama, J. Mano, Y. Inoue, and A. Kimura.: "Thioredoxin deficiency causes the constitutive activation of yAP-1, an AP-1-like transcription factor in Saccharomyces cerevisiae."J. Biol. Chem.. 274 (40). 28459-28465 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Inoue, and A. Kimura.: "Glycolytic-methylglyoxal pathway. Molecular evolution and stress response of glyoxalase I in Saccharomyces cerevisiae."Proc. Jpn. Acad.. 75B (6). 127-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Sugiyama, S. Izawa and Y. Inoue.: "The Yap1-dependent induction of glutathione synthesis in heat shock response of Saccharomyces cerevisiae."J. Biol. Chem. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S.Izawa et al.: "Thioredoxin-deficiency causes the constitutive activation of YAP-1, on AP-1 like transcription factor in Saccharomyces cerevisiae"J. Biol. Chem. 274・40. 28459-28465 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Inoue et al.: "Genetical analysis of the function of glutathione peroxidase in oxidative Stress response of Saccharomyces cerevisiae"J. Biol. Chem. 274・38. 27002-27009 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Sugiyama,S.Izawa and Y.Inoue: "The Yapl-dependent induction of glutathione synthesis in heat shock response of Saccharomyces cerevisiae"J. Biol. Chem. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Inoue and A.Kimura: "Glycolytic-methylglyoxal pathway. Molecular evolution and Stress response of glyoxalase I in Saccharomyces cerevisiae"Proc Jpn Acad.. 75B・6. 127-132 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 井上 善晴: "Saccharomyces cerevisiae のストレス応答におけるグルタチオン代謝の遺伝生化学的研究"日本農芸化学会誌. 73・10. 1013-1021 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 真野純一、浅田浩二: "光酸素ストレスを回避する分子機構"蛋白質核酸酵素. 44・15. 2239-2245 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 真野純一、木村光: "ラジカル測定による植物のストレス診断と応用"京都大学食糧科学研究所報告. 62. 26-27 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Inoue,et al: "Expression of the glyoxalase I gene of Saccharomyces cerevisiae is regulated by high osmolarity glycerol mitogen activated protein kinase pathway" Journal of Biological Chemistry. 273(5). 2977-2983 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Izawa et al: "Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae" Biochemical Journal. 330(2). 811-817 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Inoue,et al: "Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae" Biochimica et Biophysica Acta. 1395. 315-320 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Asada,et al.: "Molecular mechanism for relaxation of and protection from light stress" Stress Response of Photosynthetic Organisms. 37-52 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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