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Studies on biosynthesis of brassinosteroids and its regulation using mutants

Research Project

Project/Area Number 10460050
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionRIKEN (The Institute of Physical and Chemical Research)

Principal Investigator

FUJIOKA Shozo  RIKEN, Plant Functions Lab., Senior Research Scientist, 植物機能研究室, 先任研究員 (60165355)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordsplant hormone / brassinosteroid / plant growth and development / biosynthesis / dwarfism / brassinosteroid biosynthesis mutant / Arabidopsis thaliana / brassinosteroid insensitive mutant / 生合成・代謝 / 矮性変異体 / dwf4 / fackel / BAS1 / BRI1 / プラシノステロイド / プラシノステロイド生合成変異体 / アラビドプシス
Research Abstract

All of the brassinosteroid (BR) dwarf mutants isolated so far exhibit a short robust stature, dark-green leaves, and reduced fertility. In addition, most of the BR dwarfs display prolonged development, and exhibit varying degrees of de-etiolation when grown in the dark. Studies on BR mutants of Arabidopsis have been carried out to better understand BR biosynthesis and its regulation.
Biochemical and molecular analyses disclosed defective steps of BR biosynthetic mutants, and functions of the genes. The genes encoding many enzymes in the biosynthesis of BRs, FACKEL, DWF7, DWF5, DIM (DWF1), DET2, and DWF4 were shown to be involved in the C14-reduction, C5-desaturation, C7-reduction, C24-reduction, C5α-reduction, and C22α-hydroxylation, respectively. In addition, dwarfism in the above BR biosynthetic mutants was found to be due to a deficiency of bioactive BRs, indicating that BRs are required for proper growth and development. BR-insensitive mutants, bril, were also characterized. We showed that bril mutants accumulate very large amounts of BRs, and that the levels of BRs are positively correlated with allele and phenotypic severity, suggesting that BRI1 is required for the homeostasis of endogenous BR levels. Furthermore, bas1 mutant (phyB activation-tagged suppressor) was analyzed. The adult phenotype of bas1 mimics that of BR biosynthetic and response mutants. Dwarfism in bas1 was shown to be due to an inactivation of brassinolide by means of hydroxylation. The study also revealed that BAS1 represents one of the control points between multiple photoreceptor systems and BR signal transduction.
In conclusion, the present study disclosed the functions of the genes involved in BR biosynthsis, catabolism and signal trasduction. The study also provided the compelling evidence that BRs are essential plant hormones for proper growth and development in higher plants.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Choe,Noguchi,Fujioka,Takatsuto,Tissier,Gregory,Ross, et al.: "The Arabidopsis dwf7/ste1 mutant is defective in the Δ^7 sterol C-5 desaturation step leading brassinosteroid biosynthesis."Plant Cell. 11(2). 207-221 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Choe,Dilkes,Gregory,Ross,Yuan,Noguchi,Fujioka,Takatsuto, et al.: "The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis."Plant Physiol.. 119(3). 897-907 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seto,Fujioka,Koshino,Yoshida,Tsubuki,Honda: "Synthesis and biological evaluation of extra-hydroxylated brassinolide analogs."Tetrahedron. 55(28). 8341-8352 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noguchi,Fujioka,Takatsuto,Sakurai,Yoshida,Li,Chory: "Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5α-cholestan-3-one in brassinosteroid biosynthesis."Plant Physiol.. 120(3). 833-839 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noguchi,Fujioka,Choe,Takatsuto,Yoshida,Yuan,Feldmann,Tax: "Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids."Plant Physiol.. 121(3). 743-752 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Neff,Nguyen,Malancharuvil,Fujioka,Noguchi,Seto, et al.: "BAS1 : A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis."Proc.Natl.Acad.Sci.USA. 96(26). 15316-15323 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka,Noguchi,Watanabe,Takatsuto,Yoshida: "Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus."Phytochemistry. 53(5). 549-553 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Choe,Tanaka,Noguchi,Fujioka,Takatsuto,Ross,Tax, et al.: "Lesions in the sterol Δ^7 reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis."Plant J.. 21(5). 431-443 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seto,Fujioka,Koshino,Takatsuto,Yoshida: "Stereo and chemical course of acid catalyzed double bond migration of cholesta-5,7-dien-3β-ol to 5α-cholesta-8,14-dien-3β-ol."J.Chem.Soc.Perkin Trans.1. (11). 1697-1703 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Jang,Fujioka,Tasaka,Seto,Takatsuto,Ishii,Aida, et al.: "A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana."Genes & Development. 14(12). 1485-1497 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seto,Fujioka,Takatsuto,Koshino,Shimizu,Yoshida: "Synthesis of 6-oxy functionalized campest-4-en-3-ones : efficient hydroper-oxidation at C-6 of campest-5-en-3-one with molecular oxygen and silica gel."Steroids. 65(8). 443-449 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Noguchi,Fujioka,Choe,Takatsuto,Tax,Yoshida,Feldmann: "Biosynthetic pathaways of brassinolide in Arabidopsis."Plant Physiol.. 124(1). 201-209 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka,Noguchi,Sekimoto.Takatsuto,Yoshida: "28-Norcastasterone is biosynthesized from castasterone."Phytochemistry. 55(2). 97-101 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wang,Seto,Fujioka,Yoshida,Chory: "BRI1 is a critical component of a plasma membrane receptor for plant steroids."Nature. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 藤岡昭三,横田孝雄: "ブラシノステロイドの生合成と情報伝達「植物ホルモンのシグナル伝達」"秀潤社. 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujioka,S.: "Natural Occurrence of Brassinosteroids in the Plant Kingdom In Brassinosteroids : Steroidal Plant Hormones"Springer-Verlag. 25 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Choe, T.Noguchi, S.Fujioka, S.Takatsuto, C.P.Tissier, B.D.Gregory, A.S.Ross, A.Tanaka, S.Yoshida, F.E.Tax, and K.A.Feldmann: "The Arabidopsis dwf7/ste1 mutant is defective in the Δ^7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis."Plant Cell. 11(2). 207-221 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Choe, B.P.Dilkes, B.D.Gregory, A.S.Ross, H.Yuan, T.Noguchi, S.Fujioka, S.Takatsuto, A.Tanaka, S.Yoshida, F.E.Tax, and K.A.Feldmann: "The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis."Plant Physiol.. 119(3). 897-907 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Seto, S.Fujioka, H.Koshino, S.Yoshida, M.Tsubuki, and T.Honda: "Synthesis and biological evaluation of extrahydroxylated brassinolide analogs."Tetrahedron. 55(28). 8341-8352 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Noguchi, S.Fujioka, S.Takatsuto, A.Sakurai, S.Yoshida, J.Li, and J.Chory: "Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5α-cholestan-3-one in brassinosteroid biosynthesis."Plant Physiol.. 120(3). 833-839 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Noguchi, S.Fujioka, S.Choe, S.Takatsuto, S.Yoshida, H.Yuan, K.A.Feldmann, and F.E.Tax: "Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids."Plant Physiol.. 121(3). 743-752 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.M.Neff, S.M.Nguyen, E.J.Malancharuvil, S.Fujioka, T.Noguchi, H.Seto, M.Tsubuki, T.Honda, S.Takatsuto, S.Yoshida, and J.Chory: "BAS1 : A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis."Proc.Natl.Acad.Sci.USA. 96(26). 15316-15323 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fujioka, T.Noguchi, T.Watanabe, S.Takatsuto, and S.Yoshida: "Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus."Phytochemistry. 53(5). 549-553 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Choe, A.Tanaka, T.Noguchi, S.Fujioka, S.Takatsuto, A.S.Ross, F.E.Tax, S.Yoshida, and K.A.Feldmann: "Lesions in the sterol Δ^7 reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis."Plant J.. 21(5). 431-443 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Seto, S.Fujioka, H.Koshino, S.Takatsuto, and S.Yoshida: "Stereo and chemical course of acid catalyzed double bond migration of cholesta-5,7-dien-3β-ol to 5α-cholesta-8,14-dien-3β-ol."J.Chem.Soc.Perkin Trans.. 1(11). 1697-1703 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.-C.Jang, S.Fujioka, M.Tasaka, H.Seto, S.Takatsuto, A.Ishii, M.Aida, S.Yoshida, and J.Sheen: "A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana."Genes & Development. 14(12). 1485-1497 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Seto, S.Fujioka, S.Takatsuto, H.Koshino, T.Shimizu, and S.Yoshida: "Synthesis of 6-oxy functionalized campest-4-en-3-ones : efficient hydroperoxidation at C-6 of campest-5-en-3-one with molecular oxygen and silica gel."Steroids. 65(8). 443-449 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Noguchi, S.Fujioka, S.Choe, S.Takatsuto, F.E.Tax, S.Yoshida, and K.A.Feldmann: "Biosynthetic pathaways of Brassinosteroid in Arabidopsis."Plant Physiol.. 124(1). 201-209 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Fujioka, T.Noguchi, M.Sekimoto, S.Takatsuto, and S.Yoshida: "28-Norcastasterone is biosynthesized from castasterone."Phytochemistry. 55(2). 97-101 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Z.Wang, H.Seto, S.Fujioka, S.Yoshida, and J.Chory: "BRI1 is a critical component of a plasma membrane receptor for plant steroids."Nature. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Choe,Tanaka,Noguchi,Fujioka,Takatsuto,Ross,Tax, et al.: "Lesions in the sterol Δ^7 reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis."Plant J.. 21. 431-443 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Seto,Fujioka,Takatsuto,Koshino,Shimizu,Yoshida: "Synthesis of 6-oxy functionalized campest-4-en-3-ones : efficient hydroper-oxidation at C-6 of campest-5-en-3-one with molecular oxygen and silica gel."Steroids. 65. 443-449 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Jang,Fujioka,Tasaka,Seto,Takatsuto,Ishii,Aida, et al.: "A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana."Genes & Development. 14. 1485-1497 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Seto,Fujioka,Koshino,Takatsuto,Yoshida: "Stereo and chemical course of acid catalyzed double bond migration of cholesta-5,7-dien-3β-ol to 5α-cholesta-8, 14-dien-3β-ol."J.Chem.Soc.Perkin Trans.1. 1697-1703 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Noguchi,Fujioka,Choe,Takatsuto,Tax,Yoshida,Feldmann: "Biosynthetic pathaways of brassinolide in Arabidopsis."Plant Physiol.. 124. 201-209 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujioka,Noguchi,Sekimoto,Takatsuto,Yoshida: "28-Norcastasterone is biosynthesized from castasterone."Phytochemistry. 55. 97-101 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Choe,Dilkes,Gregory,Ross,Yuan,Noguchi,Fujioka,Takatsuto,Tanaka et al.: "The Arabidopsis dwarfl mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis"Plant Physiol.. 119. 897-907 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi,Fujioka,Takatsuto,Sakurai,Yoshida,Li,Chory: "Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-en-3-one to (24R)-24-methyl-5α-cholestan-3-one in brassinosteroid biosynthesis"Plant Physiol.. 120. 833-839 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi,Fujioka,Choe,Takatsuto,Yoshida,Yuan,Feldmann,Tax: "Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids"Plant Physiol.. 121. 743-752 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Neff,Nguyen,Malancharuvil,Fujioka,Noguchi,Seto,Tsubuki,Honda et al.: "BAS1 : A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis"Proc.Natl.Acad.Sci.USA. 96. 15316-15323 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Seto,Fujioka,Koshino,Yoshida,Tsubuki,Honda: "Synthesis and biological evaluation of extra-hydroxylated brassinolide analogs"Tetrahedron. 55. 8341-8352 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujioka,Noguchi,Watanabe,Takatsuto,Yoshida: "Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus"Phytochemistry. 53. 549-553 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤岡昭三: "ステロイド系植物ホルモンプラシノステロイドの生合成とシグナル伝達" 化学と生物. 36・11. 695-697 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Choe, S., Noguchi, T., Fujioka, S., et al.: "The Arabidopsis dwf7/ste1 Mutant is Defective in the Δ^7 Sterol C-5 Desaturation Step Leading to Brassinosteroid Biosynthesis." Plant Cell. 11. 207-221 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Choe, S., Dilkes, B.P., Gregory, B.D., et al.: "The Arabidopsis dwarf1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis." Plant Physiol.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤岡昭三、横田孝雄: "プラシノステロイドの生合成と情報伝達" 「植物ホルモンのシグナル伝達」「細胞工学」別冊 植物細胞工学シリーズ10 秀潤社, 10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujioka, S.: "Natural Occurrence of Brassinosteroids in the Plant Kingdom" Brassinosteroids:Steroidal Plant Hormones, A.Sakurai, T.Yokota, S.D.Clouse(eds.), Springer-Verlag, 25 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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