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Molecular basis for establishing the environment in digestive tract by food components

Research Project

Project/Area Number 10460052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KAWABATA Jun  Hokkaido Univ., Grad. School of Agr., Asso. Pro., 大学院・農学研究科, 助教授 (60142197)

Co-Investigator(Kenkyū-buntansha) ISHIZUKA Satoshi  Hokkaido Univ., Grad. School of Agr., Inst., 大学院・農学研究科, 助手 (00271627)
KURIHARA Hideyuki  Hokkaido Univ., Grad. School of Fis., Asso. Pro., 大学院・水産学研究科, 助教授 (40234570)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1999: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥8,300,000 (Direct Cost: ¥8,300,000)
Keywordsmaltase / α-amylase / ellagitannin / β-glucuronidase / seafood / nucleic acid / aberrant crypt / intrarectal injection / 小腸グルコシダーゼ / 酵素阻害物質 / Caco-2 / タンニン / 水産食品 / 二次胆汁酸 / 大腸ガン / 脂肪酸 / 酪酸
Research Abstract

As the inhibitory principles against rat intestinal maltase, two ellagitannins, eugeniin and casuarictin, were isolated from the methanolic extracts of Clove buds. Structure-activity relationship study of the isolates together with synthetic pentagalloylglucose (PGG), which showed the highest activity , revealed that the inhibitory activity depended on the number of uncoupled galloyl residues in the molecule. These tannins also inhibited maltase expressed on a human carcinoma cell line, Caco-2. On the other hand, the acetone extracts of roselle tea, dried flower buds of Hibiscus sabdariffa, possessed an inhibitory activity against porcine pancreatic α-amylase. The active compound was identified as hibiscus acid.
Inhibitory potencies of the extracts of seafood and algae against Escherichia coli β-glucuronidase were investigated by the colorimetric method in vitro. Among them, the extracts of dried bonito (katsuobushi), the brown alga Hizikia fusiformis, and the red alga Chondria crassica … More ulis showed the inhibitory activity. The inhibitor obtained from dried bonito was disclosed to be inosinic acid. Among nucleic acid-related compounds, adenylic acid and adenosine triphosphate also inhibited the β-glucuronidase. The inhibitor obtained from H. fusiformis was identified to be a mixture of free fatty acids. Isolation and structural elucidation of the inhibitor of C. crassicaulis is now in progress.
Direct effect of molecules on the formation of aberrant crypt foci (ACF) induced by 1,2-dimethylhydrazine using the intrarectal injection was investigated. Butyric acid or deoxycholic acid (and the derivatives) was used as the test molecule. Intrarectal injection of butyric acid showed a protective effect on the ACF formation at 20 mM. On the other hand, intrarectal injection of deoxycholic acid significantly promoted the size of ACF. These results suggest that the intrarectal injection with experimental animal is useful model to assess the effect of such molecules on the early phase of carcinogenesis. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Hideyuki Kurihara: "Inhibitory Potencies of Bromophenols from Rhodomelaceae Algae against α-glucosidase Activity"Fisheries.Sci.. 65. 300-303 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroyuki Tohda: "Inhibition of β-glucuronidase by purine nucleotides"Fisheries.Sci.. 65. 667-668 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoshi Ishizuka: "Ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt cocci in the rat colon under an apoptosis-suppressed condition"Carcinogenesis. 20. 1005-1009 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miou Toda: "β-Glucosidase Inhibitors from Clove (Syzgium aromaticum)"Biosci.Biotechnol.Biochem.. 64. 294-298 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chanida Hansawasdi: "α-Amylase Inhibitors from Roselle (Hibiscus sabdariffa Linn.) Tea"Biosci.Biotechnol.Biochem.. 64(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takumi Nagai: "Dietary Sugar beet fiber prevents an increase in a berrant crypt foci induced by gamma iradiation in the colorectum of the rat treated with an immunosuppressant"J.Nutl.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideyuki Kurihara: "Inhibitory Potencies of Bromophenols from Rhodomelaceae Algae against α-glucosidase Activity"Fisheries Sci.. 65. 300-303 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroyuki Tohda: "Inhibition of β-glucuronidase by purine nucleotides"Fisheries Sci. 65. 667-668 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoshi Ishizuki: "Ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt foci in the rat colon under an apoptosis-suppressed condition"Carcinogenesis. 20. 1005-1009 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miou Toda: "α-Glucosidase Inhibitors from Clove (Syzgium aromaticum)"Biosci. Biotechnol. Biochem.. 64. 294-298 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chanida Hansawasdi: "α-Amylase Inhibitors from Roselle (Hibiscus sabdanffa Linn.) Tea"Biosci. Biotechnol. Biochem. 64(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takumi Nagai: "Dietary sugar beet fiber prevents an increase in aberrant crypt foci induced by gamma irradiation in the colorectum of the rat treated with an immunosuppressant"J. Nutr.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miou Toda: "α-Glucosidase lnhibitors from clove (Syzgium aromaticum)"Biosci. Biotechnol. Biochem.. 64(2). 294-298 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chanida Hansawasdi: "α-Amylase Inhibitors from Roselle (Hibiscus sabdariffa Linn.) Tea"Biosci. Biotechnol. Biochem.. 64. 印刷中 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideyuki Kurihara: "Two new bromophenols from the red alga Odonthalia corymbifera"J. Nat. Prod.. 62(6). 882-884 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroyuki Tohda: "Inhibition of β-glucuronidase by purine nucleotides"Fisheries Sci.. 65(4). 667-668 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoshi Ishizuka: "Ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt foci in the rat colon under an apoptosis-suppressed condition"Carcinogenesis. 20(6). 1005-1009 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takumi Nagai: "Dietary sugar beet fiber prevents an increase in aberrant crypt foci induced by γ irradiation in the colorectum of the rat treated with an immunosuppressant"J. Nutr.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tetsuo Nishioka: "Baicalein,an α-Glucosidase Inhibitor from ScuTellaria baicalensis" J.Nat.Prod.61・11. 1413-1415 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hideyuki Kurihara: "Antibacterial activity against cariogenic bacteria and inhibition of insoluble glucan production by free fatty acids obtained from dried Gloiopellis furcata" Fisheries Sci.65・1. 129-132 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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