| Project/Area Number |
10460072
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
OKUYAMA Takashi Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Professor, 大学院・生命農学研究科, 教授 (00023482)
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| Co-Investigator(Kenkyū-buntansha) |
TERAZAWA Minoru Hokkaido University, Gradute School of Agriculture, Professor, 大学院・農学研究科, 教授 (50003124)
YOSHIDA Masato Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Assistant, 大学院・生命農学研究科, 助手 (30242845)
YAMAMOTO Hiroyuki Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Asociate-Prof., 大学院・生命農学研究科, 助教授 (50210555)
渡辺 拡 名古屋大学, 大学院・生命農学研究科, 助手 (20293714)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥8,600,000 (Direct Cost: ¥8,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | cell wall thickening / microfibrils / glucomannan / diurnal periodieity / SEM-EDX / turgor pressure / cell wall / immunogold labelling / FE-SEM / 光周期 / キシラン抗体 / 接線ひずみ / リグニン / あて材 |
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| Research Abstract |
The objective of this research is to investigate the role of the diurnal change of turgor pressure in the newly formed cells on the cell wall formation. That is, the shrinkage/expansion of the cell wall due to the turgor pressure change cases an influence to the deposition of the lignin and cellulose.
In this fiscal year, the final year of this research, we succeeded in visualizing the diurnal difference in the deposition of cell wall substance.
The differentiating cells were collected from Sugi, Cryptomeria japonica D.Don planted in the nursery in the campus of Nagoya University, at AM 6 : 00 when the tangential strain on the inner bark showed the maximum expansion. In the similar way, the cambium cells were collected at PM 4 : 00 when the tangential strain reached its minimum strain. Both sample were subjected to the SEM-EDX analysis after selective staining of glucomannan by means of an immunogold labelling method.
The result showed that the microfibrils were clearly observed on the inner surface of differentiating tracheid of the specimens collected at daytime. That is no gold particles were observed. While, on the inner surface of tracheid in the specimen prepared at am 6 : 00, many gold particles adhered to the amorphous substance were clearly observed. Microfibrils formed in the daytime were not visible due to the amorphous materials.
As the conclusion, diurnal periodicity was confirmed in the deposition of cell wall substance. That is, cellulose is deposited on the cell walls in the daytime, while hemi-celluloses are deposited in the nighttime.
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