• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism for lipoprotein transport system of eel

Research Project

Project/Area Number 10460095
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKagoshima University

Principal Investigator

HAYASI Seiichi  Kagoshima University, Faculty of Fisheries, Professor, 水産学部, 教授 (80041721)

Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsHDL-binding protein / VLDL-binding protein / eel hepatocytes / eel muscle cells / lipoproteins / vigilin / vitellogenin-mRNA / monoclonal antibodies / ビテロゲニン / RNA-結合蛋白質 / ウナギ / HDL結合タンパク質 / HDL / 脂質輸送 / vigilin / RNA結合タンパク質 / 肝臓 / アフィニテイクロマトグラフィー / コレステロール / HDL-レセプター / モノクロナール抗体 / リポタンパク質 / apoAーI / apoA-II
Research Abstract

1. High density lipoprotein (HDL) - binding protein (HBP) : HBP is suggested to play an important role for the transport of HDL to liver. cDNAs of three types of HBPs of the human being or mammals have been cloned. The HBP purified from the eel liver by HDL-Toyopearl was suggested to be vigilin, one of the three HBPs binding with RNA, from the affinity against apoAI and HDL. The HBP purified from the eel liver consisted of two main proteins with 14 and 14.5 kDa. Furthermore, it could bind to apoAI and HDL with high affinity, but to apoAII with low affinity. Eel 32 P-vitellogenin-mRNA bound to the HBP. Cloning of cDNAs of both proteins with 14 and 14.5 kDa, however, failed.
2 Very low density lipoprotein (VLDL)-binding protein (VBP) : Eel muscle contains lipid by 15 to 20 % of muscle weight. VLDL seems to transport lipid to the muscle and VBP is suggested to play an important role for lipid transport by VLDL. VBPs with 30.2, 34.2, and 39.5 kDa were purified from eel muscle homogenate by VLDL-Toyopearl and bound to VLDL with high affinity, but to HDL with low affinity.
3. Eel hepatocytes were co-cultured with eel muscle cells. The lipid of the lipoprotein secreted by the cultured hepatocytes suggested to be transported to the muscle cells by means of lipoprotein lipase or of VBP.
4. Monoclonal antibodies against eel serum HDL and VLDL were prepared. Anti-apoAI, -apoAII, and -apoB antibodies were obtained and they were very useful reagents for the studies described above.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Masaharu Komatsu: "Optical conditions for estradiol-17 β induces vigtellogenin synthesis by cultured hepatocytes of eel Anguilla japonica"Fisheries Science. 64. 658-659 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Djibril Ndiaye: "Monoclonal anticodies to plasma high density lipoprotein (HDL) of eel (Anguilla japonica)"Comparative Biochemistry and Physiology. PartB 125. 473-482 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hironori Katoh: "Distribution of apolipoprotein A-I and A-II in srum, secreted proteins by cultured hepatocytes, and the plasma membrane of hepatocytes in eel"Fisheries Science. 66. 579-585 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seiichi Hayashi: "Regulation of the synthesis and secretion of the lipoprotein by cultured eel (Anguilla japonica) hepatocytes"Trends in Comparative Biochemistry & Physiology. 7. 123-130 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hironori Katoh: "High density lipoprotein binding protein of eel (Anguilla japonica) liver with specificity of binding to apoAI as a ligand"Comparative Biochemistry and Physiology. PartB 129. 843-852 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kamonpan Awaiwanont: "Grouped, stacked rods and tapeta lucida in the retina of Japanese anchovy Engraulis japonicus"Fisheries Science. 67. 804-810 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Seiichi Hayashi: "Cell & Tissue Culture : Laboratory Procedures"John Willey & Sons. 23A:2.1 -23A:2.10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小澤貴和: "ウナギの科学(分担執筆)"恒星社厚生閣. 71-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 林征一: "動物細胞工学ハンドブック(分担執筆)"朝倉書店. 285-286 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komatsu, M. and Hayashi, S.: "Optical conditions for estradiol-17β induces vitellogenin synthesis by cultured hepatocytes of eel Anguilla japonica"Fisheries Science. 64. 658-659 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ndiaye, D. et al.: "Monoclonal antibodies to plasma high density lipoprotein(HDL) of eel (Anguilla japonica)"Comp. Biochem. Physiol.. PartB125. 473-482 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katoh, H. et al.: "Distribution of apolipoproteins A-I and A-II in serum, secreted proteins by cultured hepatocytes, and the plasma membrane of hepatocytes in eel"Fisheries Science. 66. 579-585 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, S. et al.: "Regulation of the synthesis and secretion of the lipoprotein by cultured eel (Anguilla japonica) hapatocytes"Trends In Comparative Biochemistry & Physiology. 7. 123-130 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katoh, H. et al.: "High density lipoprotein binding protein of eel (Anguilla japonica) liver with specificity of binding to apoAI as a ligand"Comp. Biochem. Physiol.. PartB129. 843-852 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Awaiwanont, K. et al.: "Grouped, stacked rods and tepeta lucida in the retina of Japanese anchovy Engraulis japonicus"Fisheries Science. 67. 804-810 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, S. and Komatsu, M.: "Primary culture of eel hepatocytes - Synthesis and secretion of lipoprotein"Cell & Tissue Culture : Laboratory Procedures, John Willey & Sons. 3A : 2.1-23A : 2.10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ozawa, T. and Hayashi, S.: "Cell biochemistry of eel (in Japanese)"Science of Eel, Kouseisya-kouseikaku. 71-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, S.: "Lipid metabolism in cultured eel hepatocytes (in Japanese)"Handbook of Animal Cell Technology, Asakura shoten. 285-286 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hironori Katoh: "High density lipoprotein binding protein of eel (Anguilla japonica) liver with specificity of binding to apoAI as a ligand"Comp.Biochem.Physiol.. PartB129. 843-852 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kamonpan Awaiwanont: "Grouped, stacked rods and tapeta lucida in the retina of Japanese anchovy Engraulis japonicus"Fisheries Science. 67. 804-810 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Ndiaye,H.Katoh,Y.P.Ge,N.Iijima,and S.Hayashi: "Monoclonal antibodies to plasma high density lipoprotein (HDL) of eel (Anguilla japonica)"Comparative Biochemistry and Physiology PartB. 125. 473-482 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Katoh,Y.P.Ge,D.Ndiaye,and S.Hayashi: "Distribution of apolipoproteins A-I and A-II in serum, secreted proteins by cultured hepatocytes, and the plasma membrane of hepatocytes in eel"Fisheries Science. 66. 579-585 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Hayashi,D.Ndiaye,M.Komatsu,and F.-G.Yu: "Regulation of the synthesis and secretion of the lipoprotein by cultured eel (Anguilla japonica) hepatocytes"Trends in Comparative Biochemistry & Physiology. 印刷中. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 林征一(分担執筆): "動物細胞工学ハンドブック(日本動物細胞工学会編集)"朝倉書店. 342 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Ndiaye et al.,: "Monoclonal antibodies to plasma high density lipoprotein(HDL) of eel (Anguilla japonica)."Comp.Biochem.Physiol.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Kato et al.,: "Distribution of apolipoproteins A-I and A-II in serum,secreted proteins by cultured hepatocytes,and the plasma membrane of hepatocytes in eel."Fisheries Science. 66(3)(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 小澤貴和、林 征一: "ウナギの科学-解明された謎と驚異のバイタリテイ-"恒星社厚生閣. 124 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Komatsu,M.and Hayashi,S.: "Optimal conditions for estradiol-17 β induced vitellogenin syntheis by cultured hepatocytes of eel Anguilla japoncia" Fisheries Science. 64・4. 658-659 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,S.and Komatsu,M: "Cell & Tissue Culture:Laboratory Precedures" John Wiley & Sons Ltd., 23A:2.1-23A:2.10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小澤貴和・林征一: "ウナギの科学" 恒星社厚生閣, (1999)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi