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Studies on the placental PRL-like molecules and their target cells

Research Project

Project/Area Number 10460121
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

SHIOTA Kunio  The Univ. of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (80196352)

Co-Investigator(Kenkyū-buntansha) OGURA Atsuo  A. O. Lab of Vet Sci, National Institute of Infectious Diseases, Researcher, 獣医科学部, 主任研究員
小倉 敦郎  国立感染症研究所, 獣医科学部, 主任研究員
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 1999: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1998: ¥7,900,000 (Direct Cost: ¥7,900,000)
Keywordsrat / placenta / pregnancy / PRL / PRL-H / SSP / PL-Im / baculovirus / PLP / PLP-H
Research Abstract

The placenta acts as a multi-function organ in the maintenance of pregnancy and in fetal growth. Rat placenta produces several members of the placental prolactin (PRL) family that show structural similarities to pituitary PRL. To date more than ten members of the placental PRL family have been cloned, and they are categorized into two subfamilies according to their Structure and function; the placental lactogen (PL) subfamily and like protein (PLP) subfamily. PRL exerts its biological function through the PRL receptor (PRL-R). PL subfamily members are also able to bind to the PRL-R and to stimulate the proliferation of Nb2 cells. Some members of PLP sub family do not bind to PRL-R nor do they stimulate lactogenic activity even though they are structura11 similar to PRL, implying that there may be yet unknown specific receptors for the PRL family members. To further understand the mechanisms underlying the expression of biological activities of PRL family members, we first evaluated the biological activity of recombinant PL-Im (recPL-Im) produced by means of a baculovirus/insect cell expression system The recPL-Im showed not only an ability to bind to PRL-R and express PRL-like activity, demonstrated by the Nb2 cell proliferation assay, but also showed luteotropic hormone (LH)-like activity in vitro. We then evaluated the role of N-linked oligosaccharides in the mo1ecular action of recPL-Im and found that the ability of PL-Im to bind PRL receptor is independent of the N-glycosylation although it appeared to be relevant for expressing PRL-like activity in the Nb2 cell proliferation assay. These results raised the possibility that recPL-Im may also act through unidentified receptor system besides the PRL-R. In this study we also performed of cDNAs for rat placenta-specific cytokines/ hormones, and isolated a novel member of PLP subfamily, named PLP-H, as well as a novel secretory protein, SSP.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Iwatsuki, K. et al.: "Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein H"Endocrinology. 139. 4976-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hattori, N. et al.: "Evaluation of the role of N-linked oligosaccharides in rat placental lactogen action by site-directed mutagenesis"Endocr J. 45. 659-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirosawa-Takamori, M. et al.: "Characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus/insect cell expression system"J Reprod Dev. 45. 43-50 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiota, K. et al.: "Epigenetic control of placental development: Polyploidy and tissue-specific DNA methylation status of trophoblast"Proceedings of International Workshop on Embryogenesis. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki, K. et al.: "A novel secretory protein produced by rat spongiotrophoblast"Biology of Reproduction. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki, K. et al.: "Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein H."Endocrinology. 139. 4976-4983 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hattori, N. et al.: "Evaluation of the role of N-linked oligosaccharides in rat placental lactogen action by site. directed mutagenesis."Endocr J. 45. 659-674 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirosawa-Takamori, M. et al.: "Characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus/insect cell expression system."J Reprod Dev. 45. 43-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiota, K. et al.: "Epigenetic control of placental development: Polyploidy and tissue-specific DNA methylation status of trophob1st."Proceedings of International Workshop On Embryogenesis and Implantation. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki, K. et al.: "A novel secretory protein produced by rat spongiotrophoblast."Biology of Reproduction. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Shiota et al.: "Epigenetic control of placental development : Polyploidy and tissue-specific DNA methylation status of trophoblast"Proceedings of International Workshop on Embryogenesis and Implantation. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Iwatsuki et al.: "A novel secretory protein by rat spongiotrophoblast"Biology of Reproduction. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Iwatsuki et al.: "Molecular cloning and characterization of a new member of the rat placental prolactin(PRL)family, PRL-like protein H." Endocrinology. 139(12). 4976-4983 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Hattori et al.: "Evaluation of the role of N-linked oligosaccharides in rat placental lactogen action by site-directed mutagenesis." Endocrine Journal. 45(5). 659-674 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Hirosawa-Takamori et al.: "Characterization of rat mid-pregnancy-specific placental lactogen produced by baculovirus/insect cell expression system." Journal of Reproduction and Development. 45. 43-50 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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