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Study on Phylogeny and Screening of Functional Gene Variants in the Domesticated Bovines and Bubalines, and their Related Wild Species by using DNA Analyses.

Research Project

Project/Area Number 10460144
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 生物資源科学
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

NAMIKAWA Takao  Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Professors, 大学院・生命農学研究科, 教授 (70111838)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Akira  Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Associate Professors, 大学院・生命農学研究科, 助教授 (20211724)
OZAWA Tomowo  Graduate Scool of Science, NAGOYA UNIVERSITY, Professors, 大学院・理学研究科, 教授 (80037233)
YAMAMOTO Yoshio  Hiroshima University, Faculty of Applied Biological Science, Professors, 生物生産学部, 教授 (10032103)
YAMAGATA Takahiro  Graduate School of Bioagricultural Sciences, NAGOYA UNIVERSITY, Assistant Professors, 大学院・生命農学研究科, 助手 (50242847)
松田 洋一  名古屋大学, 農学部, 助教授 (70165835)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1998: ¥6,700,000 (Direct Cost: ¥6,700,000)
KeywordsBovinae / cattle / water buffalo / karyotype / FISH / phylogeny / mitochondrial DNA / genetic differentiation / 野生種 / DNA / 機能遺伝子 / 変異
Research Abstract

1)Transferrin polymorphism in the tamaraw Bubalus mindorensis and comparison among the Asian buffaloes using polyacrylamide gelelectrophoresis.
Highly genetic polymorphism of transferrin (Tf) was found among plasma proteins or enzymes examined in the tamaraw, an endangered species of Asian buffalos. The variants TfA and TfB will be effective markers for analysis of the population structure of the animal.
2)Characterization and chromosomal distribution of satellite DNA sequences of the water buffalo Bubalus bubalis.
Satellite DNA was isolated from the animals and digested with two restriction endonucleases, that produced two fragments in the size of 1400bp and 700bp. This centromeric specific probes were effective to determine evolutionary changes of Robertsonian translocations among the Asian buffalo species.
3)Cytogenetic analysis of the tamaraw Bubalus mindorensis : A comparison of R-banded karyotype and chromosomal distribution of centromeric sattelite DNAs, telomeric sequence, and 18S- … More 28S rRNA genes with domestic water buffaloes.
The results obtained by using cytological methods showed that the lineage of the tamaraw was located close to the swamp type of buffaloes but the tamaraw karyotype was chracterized both by Robertsonian translocation between chromosome 7 and 15 and the telomere-centromere tandem fusion of the cromosome 4p and 12 of the standard karyotype of bovine.
4)Comparison of hemoglobin-beta chain polymorphisms of the native cattle from Laos, Thailand and Vietnam. Hemoglobin-beta chain (Hbb) polymorphism was examined. By using isoelectrofocusing method was descriminated HbbA, HbbB, HbbX1 and HbbX2 variants. The HbbA was common allele and showed a highly polymorphism with HbbB.The HbbX1 and HbbX2 were considered to be identical to that of wild bovine species of banteng and gayal respectively. The HbbX2 was few but the HbbX1 occuurred at 10-20%, suggesting a genetic contribution of gayal to those native cattle populations in the continental South-east Asia.
5)An assay for paternal gene flow between the taurus-type and the indicus-type of cattle in Laos and Vietnam using variation in SRY gene.
The RFLP analysis of SRY gene on Y-chromosome was established that could cleary identify the taurus-type of the SRY and the Indicus-type of it. Six % of 120 animals examined shouwed the taurus-type, and the rest indicus-type.
6)A Robertsonian translocation, rob (2 ; 28), found in Vietnamese cattle.
The rob (2 ; 28) Robertsonian translocation was found in Vietnamese cattle. The fused chromosome was considered to be identical with the largest one of gayal, Bos gaurus frontalis. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Chester D.Solis: "Transferrin polymorphism in the Tamaraw (Bubalus mindorensis) and comparison among the Asian buffaloes using polyacrylamide gel electrophoresis."Philippine Journal of Veterinary Medicine. 35(1&2). 37-40 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Characterization and chromosomal distribution of satellite DNA sequences of the water buffalo (Bubalus bubalis)."The Journal of Heredity. 90(3). 418-422 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Cytogenetic analysis of the Tamaraw (Bubalus mindorensis) : A comparison of R-banded karyotype and chromosomal distribution of centromeric satellite DNAs, telomeric sequence, and 18S-28S rRNA genes with domestic water buffaloes."The Journal of Heredity. 91(2). 117-121 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yukio Okada: "Comparison of hemoglobin-β chain polymorphisms of the native cattle from Laos, Thailand and Vietnam."Report of Society for Researches on Native Livestock. No.18. 75-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomowo Ozawa: "Molecular phylogenetic analysis of the wild boars and native domestic pigs in Laos."Report of Society for Researches on Native Livestock. No.18. 149-158 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "An assay for paternal gene flow between the Taurus-type and Indicus-type cattle in Laos and Vietnam using variation in SRY gene."Report of Society for Researches on Native Livestock. No.18. 59-64 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chester D.Solis: "Transferrin polymorphism in the Tamaraw (Bubalus mindorensis) and comparison among the Asian buffaloes using polyacrylamide gel electrophoresis."Philippine Journal of Veterinary Medicine. 35-(1&2). 37-40 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takao Namikawa: "Geographical distribution of coat-color variations and withers height in the native cattle of Vietnam."Report of Society for Researches on Native Livestock. 16. 149-154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ken Nozawa: "Coat-color polymorphism in water buffaloes of Vietnam."Report of Society for Researches on Native Livestock. 16. 131-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Characterization and chromosomal distribution of satellite DNA sequences of the water buffalo (Bubalus bubalis)."The Journal of Heredity. 90-3. 418-422 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahiro Yamagata: "Morphology and mitochondrial DNA variation of the wild musk shrews in Vietnam."Report of Society for Researches on Native Livestock. 17. 179-185 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomohiro Suzuki: "Comparative FISH mapping on Z chromosomes of chicken and Japanese quail."Cytogenetics and Cell Genetics. 87. 22-26 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahiro Yamagata: "Sequence variation and evolution of the mitochondrial DNA control region in the musk shrew, Suncus murinus."Genes and Genetic Systems. 74. 257-266 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Cytogenetic analysis of the Tamaraw (Bubalus mindorensis) : A comparison of R-banded karyotype and chromosomal distribution of centromeric satellite DNAs, telomeric sequence, and 18S-28S rRNA genes with domestic water buffaloes."The Journal of Heredity. 91-2. 117-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yukio Okada: "Comparison of hemoglobin-β chain polymorphisms of the native cattle from Laos, Thailand and Vietnam."Report of Society for Researches on Native Livestock. 18. 75-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomowo Ozawa: "Molecular phylogenetic analysis of the wild boars and native domestic pigs in Laos."Report of Society for Researches on Native Livestock. 18. 149-158 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "An assay for paternal gene flow between the Taurus-type and Indicus-type cattle in Laos and Vietnam using variation in SRY gene."Report of Society for Researches on Native Livestock. 18. 59-64 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Characterization and chromosomal distribution of a novel satellite DNA sequence of Japanese quail (Coturnix coturnix japonica)."The Journal of Heredity. 91-5. 412-415 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazuaki Tanaka: "Cytogenetic analysis of the Tamaraw (Bubalus mindorensis) : A comparison of R-banded karyotype and chromosomal distribution of centromeric satellite DNAs, telomeric sequence, and 18S-28S rRNA genes with domestic water buffaloes."The Journal of Heredity. 91(2). 117-121 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazuaki Tanaka: "A Robertsonian translocation, rob (2;28), found in Vietnamese cattle."Hereditas. 133. 19-23 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshimura,T.: "Molecular analysis of avian circadian clock genes."Molecular Brain Research. 78. 207-215 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yukio Okada: "Comparison of hemoglobin-β chain polymorphisms of the native cattle from Laos, Thailand and Vietnam."Report of Society for Researches on Native Livestock. No.18. 75-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomowo Ozawa: "Molecular phylogenetic analysis of the wild boars and native domestic pigs in Laos."Report of Society for Researches on Native Livestock. No.18. 149-158 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazuaki Tanaka: "An assay for paternal gene flow between the Taurus-type and Indicus-type cattle in Laos and Vietnam using variation in SRY gene."Report of Society for Researches on Native Livestock. No.18. 59-64 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi Amano: "Constitution of genes controlling blood ptotein types of Vietnam native water buffaloes and the phylogenetic study."Report of Society for Researches on Native Livestock. No.16. 33-47 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Amano: "Constitution of genes controlling blood ptotein types of Vietnam native cattle and their phylogenetic study."Report of Society for Researches on Native Livestock. No.16. 49-62 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takao Namikawa: "Geographical distribution of coat-color variations and withers height in the native cattle of Vietnam."Report of Society for Researches on Native Livestock. No.16. 149-154 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takao Namikawa: "Observation of the short-tail traits in the street dogs of Vietnam and the geographical distribution."Report of Society for Researches on Native Livestock. No.16. 155-160 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuaki Tanaka: "Characterization and chromosomal distribution of sattelite DNA sequences of the waterbuffalo, Bubalus bubalis."The Journal of Heredity. 70・3. 418-422 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazuaki Tanaka: "Cytogenetic analysis of the Tamaraw (Bubalus mindorensis): a comparison of R-banded karyotype of water buffaloes."The Journal of Heredity. (accepted). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takashi Amano: "Constitution of genes controlling blood ptotein types of Vietnam native water buffaloes and the phylogenetic study." Report of Society for Researches on Native Livestock. No.16. 33-47 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takashi Amano: "Constitution of genes controlling blood ptotein types of Vietnam native cattle and their phylogenetic study." Report of Society for Researches on Native Livestock. No.16. 49-62 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takao Namiakwa: "Geographical distribution of coat-color variations and withers height in the native cattle of vietnam." Report of Society for Researches on Native Livestock. No.16. 149-154 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takao Namiakwa: "Observation of the short-tail traits in the street dogs of Vietnam and the geographical distribution." Report of Society for Researches on Native Livestock. No.16. 155-160 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazuaki Tanaka: "Characterization and chromosomal distribution of sattelite DNA sequences of the waterbuffalo,Bubalus bubalis." The Journal of Heredity. (accepted). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazuaki Tanaka: "Cytogenetic analysis of the Tamaraw (Bubalus mindorensis):a comparison of R-banded karyotype of water buffaloes." The Journal of Heredity. (accepted). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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