| Project/Area Number |
10470028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SEIKI Motoharu Univ. Tokyo, Inst. Med. Sci., Prof., 医科学研究所, 教授 (10154634)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Yoshifumi Univ. Tokyo, Inst. Med. Sci., Assis. Prof., 医科学研究所, 助手 (70292852)
OKADA Akiko Univ. Tokyo, Inst. Med. Sci., Assis. Prof., 医科学研究所, 助手 (00233320)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | MMP / MT-MMP / GPI / マトリックスメタロプロテアーゼ / 膜型酵素 / がんの浸潤・転移 / MT4-MMP / 細胞外基質分解酵素 |
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| Research Abstract |
1. At the beginning of this study, four distinct membrane-type matrix metalloproteinases (MT-MMPs) has been identified. Since the fourth member, MT4-MMP, lacks signal peptide sequence in the reported DNA, we re-analyzed transcripts for MT4-MMP in human and mouse cells. In human, two types of the transcripts were identified and one corresponded to the previously reported one. The other transcript was found to encode signal peptide a part of propeptide sequence that was missing in the previously reported one. Thus, functional gene was firstly identfied. Similarly, mouse cDNA was also identified.
2. The carboxy terminal region of MT4-MMP is distantly related to other MT-MMP membaers that have a hydrophobic amino acid streatch acting transmembrane domain. Although the C-terminal amino acids of MT4-MMP is also hydrophobic, it was demonstarted that the sequence was a signal for processing and for glycosyal phosphatidial inositol anchoring.
3. Fifth member of MT-MMP group was identified by cDNA cloning. It was found to be expressed selectively in cereberum and thoght to be important for the development and maintenance of central nervous system.
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