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Structure and Functional Relationship of Band 3 Protein

Research Project

Project/Area Number 10470033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HAMASAKI Naotaka  Graduate School of Medical Sciences, Kyushu University, Department of Clinical Chemistry and Laboratory Medicine, Professor, 大学院・医学系研究科, 教授 (00091265)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥4,200,000 (Direct Cost: ¥4,200,000)
Keywordspolytopic membrane protein / biosynthesis of membrane protein / transmembrane peptide / signal anchor-I / peptide interaction / band 3 protein / anion exchange / 工型シグナルアンカー配列 / 構造と機能解析 / 膜貫通ドメイン / マルチスパン
Research Abstract

Our recent studies on band 3 protein, a typical multi-spanning polytopic membrane protein, implied that some hydrophobicpeptideportions were found as free from boundary lipids even though their hydrophobicity was comparable to that of bound from (Hamasaki, et al., J. Biochem. 122, 577-585(1997)). Moreover, the anticipated transmembrane peptide portions of band 3 protein were not equivalent to each other and some of them had no topogenic signal activities (Ota, et al., Biol. Chem. 273, 28286-28291(1998)). It was also found that topogenic signal activities were affected by the mutual peptide-peptide interactions of the transmembrane peptide portions during biosynthesis in vitro (Ota, et al., Biol. Chem. 273, 28286-28291(1998)). Intriguingly, we also observed the evidence for a mode of co-translational insertion in which an internal signal-another sequence with Nexo/Ccyt topology conferred a transmembrane disposition onto a preceding hydrophilic peptide segment (Ota, et al., Molecular Cel … More l 2, 495-503(1998)), suggesting that hydrophilic peptide portions can transverse the membrane lipid bilayer surrounded by other trasnmembrane peptide portions (especially with amphipathic transmembrane peptide portions). It is highly probable to speculated that the mutual peptide-peptide interactions of multi-spanning polytopic membrane proteins occurred throughout the protein biosynthesis and plasma membrane intersection events (Hamasaki, et al., Biochem. Cell., Biol. 76, 729-733(1998)).
Based on this evidence, it can be concluded that the hydrophobic transmembrane peptide portions are not necessarily bound with boundary lipids in the membrane lipid bilayer. Thus, hydrophobicity is not an absolute requirement for the formation of a transmembrane segment in multispanning polytopic membrane proteins. Category 2 portions (see J. Biochem. 122, 577-585 (1997) ; Biochem. Cell Biol. 79, 729-733 (1998)) have considerable freedom in the membrane lipid bilayer. Proteins containing category 2 portions have a more flexible structure than membrane proteins that consist only of transmembrane peptide portions bound with boundary lipids. These flexible regions must play important roles in substrate-peptide interactions or ligand-mediated conformational change within membrane lipid bilayers. Further study on other polytopic membrane proteins would support this new concept. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Naotaka Hamasaki: "The Role of Band 3 Protein in Oxygen Delivery by Red Blood Cells."Ind. J. Clin. Biochem.. 14(1). 49-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Naotaka Hamasaki: "A New Conceptin Polytopic Membrane Proteins Following from the Study of Band 3 Protein"Biochem. Cell. Biol.. 76(5). 729-733 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 濱崎 直孝: "内在性膜蛋白質の新しいコンセプト"生物物理. 39(4). 240-245 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiromicichi Mitsuyasu: "Dominant Effector Ile50 Val Variant of the Human IL-4 Receptora-Chain in IgE synthesis."J. Immunol.. 162. 1227-1234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroko Tsuda: "Screening for Aetiology of Thrombophilia: A High Prevalence of Protein S Abnormality."Ann. Clin. Biochem.. 36. 423-432 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideki Tatewaki: "A Novel Splice Acceptor Site Mutation Which Produces Multiple Splicing Abnormalities Resulting in Protein S Deficiency Type I."Thromb. Haemost.. 82(1). 65-71 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuma, H., Inoue, K., Fu., G., Ando, S., Lee, S., Sugihara, G., Hamasaki, N.: "Secondary Structure of Synthetic Peptides Corresponding to the First Membrane - Contact Portion of Normal Band 3 and its Deletion Mutant (Southeast Asian Ovalocytosis)"J. Biochem.. 124(3). 509-518 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ota, K., Sakaguchi, M., Hamasaki, N., Mihara, K.: "Assessment of Topogenic Functions of Anticipated Transmembrane Segments of Human Band 3"J. Biol. Chem.. 273(43). 28286-28291 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ota, K., Sakaguchi M., von Heijine, G., Hamasaki, N., Mihara, K.: "Forced Transmembrane Orientation of Hydrophilic Polypeptide Segments in Multispanning Membrane Proteins"Molecular Cell. 2. 495-503 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hamasaki N.: "The Role of Band 3 Protein in Oxygen Delivery by Red Blood Cells (Review Articles)"Ind. J. Clin. Biochem.. 14(1). 49-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hamasaki N., Kuma, H., Kazuhisa Ota, K., Masao Sakaguchi, M., Mihara, K.: "A new Concept in Polytopic Membrane Proteins Following From the Study of Band 3 Protein"Biochem.Cell Biol.. 76. 729-733 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Naotaka Hamasaki: "The Role of Band3 Protein in Oxygen Delivery by Red Blood Cells."Ind. J. Clin. Biochem.. 14(1). 49-58 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naotaka Hamasaki: "A New Conceptin Polytopic Membrane Proteins Following from the Study of Band3 Proten."Biochem. Cell Biol.. 76(5). 729-733 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiromichi Mitsuyasu: "Dominant Effect of Ile50Val Variant of the Human IL-4 Receptora-Chain in IgE Svnthesis."J. Immunol.. 162. 1227-1234 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroko Tsuda: "Screening for Aetiology of Thrombophilia: A High Prevalence of Protein S Abnormality."Ann. Clin. Biochem.. 36. 423-432 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideki Tatewaki: "A Novel Splice Acceptor Site Mutation Which Produces Multiple Splicing Abnormalities Resulting in Protein S Deficiency Type I."Thromb. Haemost.. 82(1). 65-71 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 濱崎直孝: "内在性膜蛋白質の新しいコンセプト"生物物理. 39(4). 240-245 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hamasaki,N.: "Band 3 Protein:Physiology, Function and Structure" Cell.Mol.Biol.42・(7). 1025-1039 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hamasaki,N.: "Proteolytic Cleavage Sites of Band 3 Protein in Alkali-treated Membranes: Fidelity of Hydropathy Prediction on Band 3 Protein." J.Biochem.122(3). 577-585 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuma,H.: "Secondary Structure of Synthetic Peptides Corresponding to the First Membrane-Contact Portion of Normal Band 3 and its Deletion Mutant(Southeast Asian Ovalocytosis)" J.Biochem.124(3). 509-518 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ota,K.: "Assessment of Topogenic Functions of Anticipated Transmembrane Segments of Human Band 3" J.Biol.Chem.273(43). 28286-28291 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ota,K.: "Forced Transmembrane Orientation of Hydrophilic Polypeptide Segments in Multispanning Membrane Proteins" Molecular Cell.2. 495-503 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hamasaki,N.: "A New Concept in Polytopic Membrane Proteins Following from the Study of Band 3 Protein." Biochem.Cell.Biol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hamasaki,N.: "Membrane Proteins: Structure,Function and Expression Control" Kyushu University Press(Fukuoka)/Karger Medical and Scientific Publishers (Basel)(eds. by Hamasaki,N.& Mihara,K), 413 (1997)

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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