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Macrophage scavenger receptor as a new macrophage marker

Research Project

Project/Area Number 10470061
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKumamoto University

Principal Investigator

TAKEYA Motohiro  SCHOOL OF MEDICINE, Kumamoto University, PROF., 医学部, 教授 (90155052)

Co-Investigator(Kenkyū-buntansha) MIYAKAWA Kazuhisa  SCHOOL OF MEDICINE, Kumamoto University, ASSISTANT, 医学部, 助手 (00244111)
TAKAHASHI Kiyoshi  SCHOOL OF MEDICINE, Kumamoto University, PROF., 医学部, 教授 (70045631)
SAKASHITA Naomi  SCHOOL OF MEDICINE, Kumamoto University, ASSIS PROF., 医学部, 講師 (90284752)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥6,000,000 (Direct Cost: ¥6,000,000)
Keywordsmacrophage marker / scavenger receptors / monoclonal antibodies / tissue macrophages / immunohistochemistry / pathological tissues / paraffin sections / CD204 / ノックアウトマウス / 単球分化 / 動物組織 / 免疫組織科学 / ヒト組織
Research Abstract

Macrophage scavenger receptor (MSR) is one of the major receptors of macrophages and play important roles in atherogenesis and host defense mechanisms. Monoclonal antibodies were generated by immunizing MSR-deficient mice with a recombinant protein of human type I MSR as immunogen. Five antibodies were confirmed to be specific for MSR by Western blot analysis.
Immunohistochemically, all antibodies recognized tissue macrophages such as alveolar macrophages, Kupffer cells in the liver, red pulp macrophages in the spleen, sinus macrophages in lymph nodes, and interstitial macrophages in various organs. Perivascular macrophages in the brain (Mato cells) were also positive for these antibodies. Freshly isolated blood monocytes were negative ; however, they became positive for these antibodies after 1 day in culture. At 3 to 5 days in culture the reaction intensity became stronger along their differentiation towards macrophages. Dendritic cells such as interdigitating cells of lymphoid tissue … More s and epidermal Langerhans cells were invariably negative. In early atherosclerotic lesions, these antibodies recognized scattered macrophages in intima and foamy macrophages in the periphery of atheromatous cores. In various inflammatory conditions, these antibodies recognized infiltrated macrophages. Epithelioid cells and multinucleated giant cells in epithelioid granuloma of tuberculosis and sarcoidosis were also positive. Each antibody showed a unique reaction pattern against animal species. Especially, an antibody named SRA-E5 recognized MSR molecules of various animal species including monkeys, cows, horses, swine, beagles, cats, rabbits, guinea pigs, hamsters, rats, and mice. From these observations, these antibodies are considered to be useful not only for studying the role of MSRs but also for analyzing macrophage differentiation in human and animals. Through the evaluation of these antibodies at the 7th Workshop and Conference on Human Leucocyte Differentiation Antigens MSR has been assigned to be a new CD molecule, CD204. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Honda M et al.: "Immunohistochemical evidence for a macrophage scavenger receptor in Mato cells and reactive microglia of ischemia and Alzheimer's disease."Biochem Biophys Res Comm. 245. 734-740 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakaguchi H et al.: "Roel of macrophage scavenger receptors in diet-induced atherosclerosis."Lab Invest. 78. 423-434 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Cynshi O et al.: "Antiatherogenic effects of the antioxidant BO-653 in three different animal models."Proc Natl Acad Sci (USA). 95. 10123-10128 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hagiwara S et al.: "Role of macrophage scavenger receptors in hepatic granuloma formation in mice."Am J Pathol. 153. 128-140 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeya M et al.: "Macrophage scavenger receptors : structure, function and tissue distribution (review)."Acta Histochem Cytochem. 32. 47-51 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi K et al.: "The role of macrophage scavenger receptors in zymocel- or corynebacterium parvum-induced hepatic granuloma formation in mice."Cells of Hepatic Sinusoids. 7. 220-223 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaku M et al.: "An in vitro coculture model of transmigrant monocytes and foam cell formation."Arteriosc Thromb Vasc Biol. 19. 2330-2339 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakashita N et al.: "Localization of human acyl-coenzyme A : cholesterol acyltransferase-1(ACAT-1) in macrophages and in various tissues."Am J Pathol. 156. 227-236 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Lehtolainen P et al.: "Retrovirus-mediated, stable scavenger-receptor gene transfer leads to functional endocytotic receptor expression, foam cell formation, and increased susceptibility to apoptosis in rabbit aortic smooth muscle cells."Arterioscler Thromb Vasc Biol. 20. 52-60 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Usui M et al.: "Important role of local angiotensin II activity mediated via tyep 1 receptor in the pathogenesis of cardiovascular inflammatory changes induced by chronic blockade of nitric oxide synthesis in rats."Circulation. 101. 305-310 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi K et al.: "The role of macrophage scavenger receptors in atherogenesis."Seminars in Clin Immunol. 19. 29-38 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Koyanagi M et al.: "Role of monocyte chemoattractant protein-1 in cardiovascular remodeling induced by chronic blockade of nitric oxide synthesis."Circulation. 102. 2243-2248 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wynn AA et al.: "Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation."Am J Pathol. 158. 131-145 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeya M et al.: "Macrophage scavenger receptor, a new differentiation marker for macrophages."In Leucocyte Typing VII, White Cell Differentiation Anitgens (Mason D et al.eds), Oxford University Press. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 高橋潔 他: "生命を支えるマクロファージ"文光堂(東京)(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakaguchi H et al.: "Role of macrophage scavenger receptors in diet-induced atherosclerosis in mice"Lab Invest. 78. 423-434 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Honda M et al.: "Immunohistochemical evidence for a macrophage scavenger receptor in Mato cells and reactive microglia of ischemia and Alzheimer's disease"Biochem Biophys Res Comm. 245. 734-740 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Cynshi O et al.: "Antiatherogenic effects of the antioxidant BO-653 in three different animal models"Proc Natl Acad Sci, USA. 95. 10123-10128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hakamata, H et al.: "The very low- and intermediate-density lipoprotein fraction isolated from apolipoprotein E-knockout mice transforms macrophages to foam cells through an apolipoprotein E-independent pathway"Biochemistry. 37. 13720-13727 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ling X et al.: "Immunohistochemical distribution and subcellular localization of three distinct specific molecular structure of advanced glycation end products in human tissues"Lab Invest. 78. 1591-1606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda H et al.: "Molecular cloning, functional expression and tissue distribution of rat coenzyme A : cholestrol acyltransferase with its activation by acetylated LDL inperitoneal macrophages"Biochem Biomed Acta. 1391. 193-203 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazaki A et al.: "Expression of ACAT-1 protein in human atherosclerotic lesions and in cultured human monocyte-macrophages"Arterioscler Thromb Vasc Biol. 18. 1568-1574 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hagiwara S et al.: "Role of macrophage scavenger receptors in hepatic granuloma formation in mice"Am J Pathol. 154. 705-720 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeya M et al.: "Macrophage scavenger receptors : structure, function and tissue distribution (review)"Acta Histochem Cytochem. 32. 47-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kaikita K et al.: "Colocalization of tissue factor and tissue factor pathway inhibitor in coronary atherosclerosis"J Pathol. 188. 180-188 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaku M et al.: "An in vitro coculture model of transmigrant monocytes and foam cell formation"Arterioscler Thromb Vasc Biol. 19. 2330-2339 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakashita N et al.: "Localization of acyl-coenzyme A : cholesterol acytransferase (ACAT-1) in macrophages and other various cell types of human tissues"Am J Pathol. 156. 227-236 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Lehtolainen P et al.: "Retrovirus-mediated, stable scavenger-receptor gene transfer leads to functional endocytotic receptor expression, foam cell formation, and increased susceptibility to apoptosis in rabbit aortic smooth muscle cells"Arterioscler Thromb Vasc Biol. 20. 52-60 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Usui M et al.: "Important role of local angiotensin II activity mediated via type 1 receptor in the pathogenesis of cardiovascular inflammatory changes induced by chronic blockade if nitric oxide synthesis in rats"Circulation. 101. 305-310 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi K et al.: "The role of macrophage scavenger receptors in atherogenesis."Seminars in Clin Immunol. 19. 29-38 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wynn AA et al.: "Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation."Am J Pathol. 158. 131-145 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata N et al.: "Glycoxidation and lipid peroxidation of low-density lipoprotein can synergistically enhance atherogenesis."Cardiovasc Res. 49. 466-475 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeya M et al.: "Macrophage scavenger receptor, a new differentiation marker for macrophages"In Leucocyte Typing VII (Mason D et al.eds), Oxford University Press (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakashita N et al.: "Localization of Human Acyl-Coenzyme A : Cholesterol Acyltransferase-1(ACAT-1) in Macrophages and in Various Tissues."Am J Pathol. 156. 227-236 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Lehtolainen P et al.: "Retrovirus-mediated, stable scavenger-receptor gene transfer leads to functional endocytotic receptor expression, foam cell formation, and increased susceptibility to apoptosis in rabbit aortic smooth muscle cells."Arterioscler Thromb Vasc Biol. 20. 52-60 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Usui M et al.: "Important role of local anglotensin II activity mediated vla type 1 receptor in the pathogenesis of cardiovascular inflammatory changes induced by chronic blockade of nitric oxide synthesis in rats."Circulation. 101. 305-310 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takahashi K et al.: "The role of macrophage scavenger receptors in atherogenesis."Seminars in Clin Immunol. 19. 29-38 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koyanagi M et al.: "Role of monocyte chemoattractant protein-1 in cardiovascular remodeling induced by chronic blockade of nitric oxide synthesis."Circulation. 102. 2243-2248 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Wynn AA et al.: "Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation."Am J Pathol. 158. 131-145 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeya M et al.: "Macrophage scavenger receptor, a new differentiation marker for macrophages."In Leucocyte Typing VII, White Cell Differentiation Anitgens (Mason D et al.eds), Oxford University Press. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高橋潔 他: "生命を支えるマクロファージ"文光堂(東京)(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hagiwara S et al.: "Role of macrophage scavenger receptors in hepatic granuloma formation in mice"Am J Pathol. 153. 128-140 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeya M et al.: "Macrophage scavenger receptors: structure, function and tissue distribution (review)"Acta Histochem Cytochem. 32. 47-51 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takahashi K et al.: "The role of macrophage scavenger receptors in Zymocel-or Corynebacterium parvum-induced hepatic granuloma formation in mice"Cells of Hepatic Sinusoids. 7. 220-223 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takaku M et al.: "An in vitro coculture model of transmigrant monocytes and foam cell formation"Arteriosc Thromb Vasc Biol. 19. 2330-2339 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Lehtolainen P et al.: "Retrovirus-mediated, stable scavenger-receptor gene transfer leads to functional endocytotic receptor expression, foam cell formation, and increased susceptibility to apoptosis in rabbit aortic smooth muscle cells"Arteriosc Thromb Vasc Biol. 20. 52-60 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Honda M et al.: "Immunohistochemical Evidence for a Macrophage Scavenger Receptor in Mato cells and Reactive Microglia of Ischemia and Alzheimer's Disease" Biochem Biophys Res Comm. 245. 734-740 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Skaguchi H et al.: "Role of macrophage scavenger receptors in diet-induced atherosclerosis" Lav Invest. 78. 423-434 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Cynshi O et al.: "Antiatherogenic effects of the antioxidant BO-653 in three different animal models" Proc Natl Acad Sci USA. 95. 10123-10128 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hgiwara S et al.: "Role of macrophage scavenger receptors in hepatic granuloma formation in mice" Am J Pathol. 154. 705-720 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeya M et al.: "Macrophage scavenger receptors: structure, function and tissue distribution" Acta Histochem Cytochem. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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