A study on an aberrant differentiation into chondrocytes in progressive ankylosis (ank/ank)
| Project/Area Number |
10470062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Wakayama Medical College |
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Principal Investigator |
MURAGAKI Yasuteru Wakayama Medical College, 1st Dept.of Pathology, Associate Professor, 医学部, 助教授 (40190904)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | progressive ankylosis / positional cloning / mapping / subtraction cloning / サブトラクションクローニング / CAリピートマーカー / 軟骨 / 原因遺伝子 |
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| Research Abstract |
Progressive ankylosis (ank/ank) is an autosomal recessive skeletal disorder in mice. To investigate the precise pathological changes in the joints of ank/ank, the joint tissues were removed from ank/+ and ank/ank mice, paraffin-embedded, and examined with H.E. stained sections. In a 4 week-old ank/ank mouse, the differentiation of synovial cells into chondrocytes was seen and the synovial membrane was thickened by the proliferation of the chondrocytes. In an 8 week-old mouse, the thickened synovial membranes were partially calcified and the surface of the articular cartilage became irregular.
To map the causative gene of progressive ankylosis, we analyzed the recombination events at genetic markers in chromosome 15 using F2 homozygous mice bled with the other strain mice. Of 400 meioses, we found 6 recombinations at D15Mit130 for the minimum of recombination. We assigned the Ank locus to the 1.25 cM region between D15Mit130 and D15Mit6. To narrow down the region, we plan to find polymorphic markers with BAC clones and oligo GT primers because there is no more informative marker in the region.
In parallel with the positional cloning, the subtraction cloning was carried out. We took advantage of suppression subtractive hybridization (SSH) for the cloning. As a result, we obtained, at the present time, two positive clones that are expressed less in the tissues of ank/ank mice than those of unaffected mice. For the further study we sill extend the cDNAs to the full length and map the gene loci.
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Report
(3 results)
Research Products
(1 results)
