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Interaction of secreting pathogenic factors in vibriosis.

Research Project

Project/Area Number 10470069
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

SHINODA Sumio  Okayama University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (50029782)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Shin-ichi  Okayama University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (60182060)
TOMOCHIKA Ken-ichi  Okayama University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00093691)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsPathogenic vibrio / Protease / Hemolysin / Vibrio vulnificus / Vibrio mimicus / タンパク毒素 / ヘモリジン / エンテロトキシン
Research Abstract

Bacteria of genus Vibrio are habitant of natural aquatic environment and include some pathogenic species which secret various pathogenic factors. These factors usually cause symptoms by cooperating with or complementing to other factors. This research project was carried out to understand general mechanism of vibriosis by studying cooperative and complementary action of pathogenic factors of vibrios, such as hemolysin, protease, siderphore or enterotoxin of Vibrio vulnificus and V.mimicus.
Hemolysisn and proteases were extensively studied in this research. Proteases of vibrios causing systemic infection, such as V.vulnificus, act directly as the toxic factor by stimulating vascular permeability. However proteases of enteropathogenic vibrios, such as V.mimicus, show indirect action ; influencing the colonization mechanism by modifying intestinal membrane, activation or inactivation of protein toxins by partial digestion of the peptide for nicking some special part.
Although hemolytic action of V.mimicus hemolysin was not influenced by partial digestion with protease produced by the vibrio, enterotoxic activity of the hemolysin was lost by the proteolysis. V.vulnificus hemolysin was also nicked by partial digestion of the vibrio protease, and the influenc to the activity was different between the hemolysins of biotype 1 and 2. These results are useful information for understanding mechanism of vibrio infection.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Shinoda,S.,Miyoshi,S.: "Enteropathogenic factors produced by vibrios other cholera toxin."J.Natural Toxins.. 9. 231-249 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Bi,K.,Shi,L.,Miyoshi,S.,Shinoda,S. et al.: "Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction."Microbiol.Immunol.. 44. 149-153 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shi,I.,Miyoshi,S.,Shinoda S. et al.: "Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction."J.Health Sci.,. 46. 63-65 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyoshi,S.,Shinoda,S.: "Microbial metalloproteases and pathogenesis."Microbes & Infect.. 2. 91-98 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinoda, S., Miyoshi, S.: "Enteropathogenic factors produced by vibrios other than cholera toxin."J.Natural Toxins.. 9. 231-249 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Bi, K., Shi, L., Miyoshi, S., Shinoda, S.et al.: "Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction."Microbiol.Immunol.. 44. 149-153 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shi, L., Miyoshi, S., Shinoda S.et al.: "Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction."J.Health Sci.. 46. 63-65 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyoshi, S., Shinoda, S.: "Microbial metalloproteases and pathogenesis."Microbes & Infect.. 2. 91-98 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinoda,S.,Miyoshi,S.: "Enteropathogenic factors produced by vibrios other cholera toxin."J.Natural Toxins.. 9,. 231-249 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Bi,K.,Shi,L.,Miyoshi,S.,Shinoda,S. et al.: "Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction."Microbiol.Immunol.. 44,. 149-153 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shi,L.,Miyoshi,S.,Shinoda S. et al.: "Presence of hemolysin genes (vmh, tdh and hlx) in isolates of Vibrio mimicus determined by polymerase chain reaction."J.Health Sci.,. 46,. 63-65 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyoshi,S.,Shinoda,S.: "Microbial metalloproteases and pathogenesis."Microbes & Infect.. 2,. 91-98 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinoda, S.: "Protein toxins produced by pathogenic vibrios"J. Natural Toxins.. 8. 259-270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyoshi, S., et al.: "The ability of Vibrio vulnificus to use a synthetic hydrophilic heme-compound, Fe-TPPS, as a single iron source"FEMS Microbiol. Lett.. 172. 73-77 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyoshi, S. et al.: "The hemagglutinating action of Vibrio vulnificus metalloprotease"Microbiol. Immunol.. 43. 79-82 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Bi, K., et al.: "Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction"Microbiol. Immunol.. 44. 149-153 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shinoda,S.: "Bacterial protein toxins: a comprehensive source book, 2nd. ed,"Academic Press, London. 350 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyoshi,S.: "The hemagglutinating action of Vibrio vulnificus metalloprotease." Microbiology and Immunology. 43. 79-82 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyoshi,S.: "Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease,a member of the thermolysin family." Infection and Immunity. 66. 4851-4855 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shi,L.: "Detection of genes encoding cholera toxin (CT), Zonula occludens toxin (ZOT), accessory cholera enterotoxin (ACE) and heat-stable enterotoxin (ST) in Vibrio mimicus clinical strains." Microbiology and Immunology. 41. 823-828 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyoshi,S.: "The ability of Vibrio vulnificus to use a synthetic hydrophilic heme-compound,Fe-TPPS,as a single iron source." FEMS Microbiology Letters. 印刷中. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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