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Researches on molecular mechanisms of multicomponent efflux systems of Pseudomonas aeruginosa

Research Project

Project/Area Number 10470070
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyoto Pharmaceutical University, Faculty of Pharmaceutical Sciences

Principal Investigator

GOTOH Naomasa  Kyoto Pharmaceutical University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (30121560)

Co-Investigator(Kenkyū-buntansha) TSUDA Masataka  Tohoku University, Research Center of Genetic Ecology, Professor, 遺伝生態研究センター, 教授 (90172022)
NISHINO Takeshi  Kyoto Pharmaceutical University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (50097838)
辻元 秀人  京都薬科大学, 薬学部, 助手 (10257777)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2000: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥6,700,000 (Direct Cost: ¥6,700,000)
KeywordsPseudomonas aeruginosa / Mex efflux systems / Antimicrobial resistance / Multicomponents / Substrate specificity / Substrate recognition / Expression / Molecular structure / キメラ排出システム / アミノ酸置換 / 臨床分離株 / 多剤排出システム / 異物排出 / 免疫沈降 / トポロジー / MexC-MexD-OprJ / DNAシャフリング / キメラ / 膜蛋白質
Research Abstract

Our project has been focused on researches on molecular mechanisms of multicomponent efflux systems, which involved to broad-spectrum cross-resistance of Pseudomonas aeruginosa and obtained the following results. 1) A new efflux system MexX-MexY functions under collaboration with OprM, which expresses in the wild-type strains, such as PAO1. 2) Several antimicrobial agents such as erythromycin, tetracycline and gentamicin de-represses expression of MexX-MexY, which is suppressed in the wild-type cells. 3) All of these efflux systems extrude a wide variety of antimicrobial agent groups such as quinolones, macrolides, tetracyclines, lincomycin, chloramphenicol, most penicillins (except carbenicillin and sulbenicillin), most cephems (except cefsulodin and ceftazidime), meropenem and S-4661, but not imipenem and polymyxin B.Nonetheless, the respective substrate specificities to some agents are distinguishable. 4) The RND family proteins are assumed to function as an exporting component, alt … More hough there is little experimental evidence to support this assumption. To verify the assumption, based on the MexC-MexD-OprJ efflux system of Pseudomonas aeruginosa, we constructed eight chimeric efflux systems, in which MexD was exchanged for other RND proteins. RND proteins such as MexB and MexD are primary components of the efflux systems that function in the recognition of extrusion substrates. 5) To investigate the topology of MexD more thoroughly, 25 MexD-PhoA (alkaline phosphatase) and 18 MexD-Bla (β-lactamase) fusion plasmids were constructed and analyzed. The resulting topological model had just 12 transmembrane helices and two periplasmic loops of about 300 residues between helices 1 and 2 and helices 7 and 8. It is therefore proposed that the N and C termini are located in the cytoplasm and the predicted orientation is consistent with the "positive-inside rule". This topological model can be applied to other RND proteins. Furthermore, analysis of mutated MexD from the clinical isolates showed that the two periplasmic loops function to recognition of substrates. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (75 results)

All Other

All Publications (75 results)

  • [Publications] 後藤直正: "緑膿菌の多剤耐性化に寄与する薬剤排出システム"日本化学療法学雑誌. 47. 319-329 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 後藤直正: "キノロン耐性-複数の機構の総和"医学のあゆみ. 191. 1031-1035 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 後藤直正: "細菌の異物(薬剤)排出蛋白質と抗菌薬耐性"日本化学療法学雑誌. 48. 509-515 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 後藤直正: "膜透過および排出機構に伴う多剤耐性菌"日本臨牀. 59. 712-718 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] R.Srikumar: "Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MoxB-OprM and MoxC-MoxD-OprJ in Eschorichia coli."Antimicrob.Agents Chemother.. 42. 65-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 川畑敏枝: "NalB型排出蛋白質をもつ緑膿菌に対するFOMとβ-ラクタム薬の併用効果"日本化学療法学雑誌. 46. 17-21 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Yoneyama: "Subunit swapping in the Mex-extrusion pumps in Pseudomonas aeruginosa."Biochem.Biophys.Res.Commun.. 244. 898-902 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Characterization of the MexC-MexD-OprJ multidrug efflux system in ΔmexA-mexB-oprM mutants of Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 42. 1938-1943 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa."FEMS Microbiol.Lett.. 165. 21-27 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda: "Interplay between Chromosomal β-lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to β-lactams in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 43. 400-402 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyomi Okamoto: "Molecular cloning of oprQ encoding to the outer membrane protein OprE3 of Pseudomonas aeruginosa."Microbiol.Immunol.. 43. 297-301 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 高山吉弘: "nfxB型排出蛋白をもつPseudomonas aeruginosaに対するfosfomycinとcefepimeの併用効果."日本化学療法学雑誌. 47. 264-270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Topological analysis of an RND family transporter, MexD of Pseudomonas aeruginosa."FEBS Lett.. 458. 32-36 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shah Jalal: "Rapid identification of mutations in a multidrug efflux pump in Pseudomonas aeruginosa."APMIS. 107. 1109-1116 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideto Tsujimoto: "Diffusion of macrolide antibiotics through the outer membrane of Moraxella catarrhalis."J.Infect.Chemother.. 5. 196-200 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shah Jalal: "Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients."Antimicrob.Agents Chemother.. 44. 710-712 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda: "Contribution of the MexX-MexY-OprM Efflux System to Intrinsic Resistance in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 44. 2242-2246 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda: "Substrate Specificity of the MexAB-OprM, MexCD-OprJ, and MexXY-OprM Efflux Pumps in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 44. 3322-3327 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda: "Hypersusceptibility of the Pseudomonas aeruginosa nfxB mutant to β-lactams due to reduced expression of AmpC β-lactamase."Antimicrob.Agents Chemother.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hyunjoo Pai: "Carbapenem resistance mechanisms in Pseudomonas aeruginosa clinical isolates."Antimicrob.Agents Chemother.. 45. 480-484 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Multidrug resistance mechanisms in Pseudomonas aeruginosa"Chemotherapy (Tokyo). 47. 17-21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nsaomasa Gotoh: "Resistance mechanisms-Interplay among resistance mechanisms"Igakunoayumi. 91. 1031-1035 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Bacterial xenobiotic (antibiotic) efflux proteins and antibiotic resistance"Chemotherapy (Tokyo). 48. 509-515 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh: "Antibiotic resistance caused by membrane impermeability and multidrug efflux systems"Nipponrinsho. 59. 712-718 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ramakrishnan Srikumar et al.: "Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-OprJ in Escherichia coli."Antimicrob.Agents Chemother.. 42. 65-71 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshie Kawabata et al.: "Synergy effects of FOM and □-lactams to NfxB mutants of Pseudomonas aeruginosa."Chemotherapy (Tokyo). 46. 17-21 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Yoneyama et al.: "Subunit swapping in the Mex-extrusion pumps in Pseudomonas aeruginosa."Biochem.Biophys.Res.Commun.. 244. 898-902 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh et al.: "Characterization of the MexC-MexD-OprJ multidrug efflux system in Δ mexA-mexB-oprM mutants of Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 42. 1938-1943 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh et al.: "Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa."FEMS Microbiol.Lett.. 165. 21-27 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda et al.: "Interplay between Chromosomal β-lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to β-lactams in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 43. 400-402 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kiyomi Okamoto et al.: "Molecular cloning of oprQ encoding to the outer membrane protein OprE3 of Pseudomonas aeruginosa."Microbiol.Immunol.. 43. 297-301 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihiro Takayama et al.: "Synergy effects of fosfomycin and □-cefepime to nfxB mutants of Pseudomonas aeruginosa."Chemotherapy (Tokyo). 47. 264-270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naomasa Gotoh et al.: "Topological analysis of an RND family transporter, MexD of Pseudomonas aeruginosa"FEBS Lett, .. 458. 32-36 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shah Jalal et al.: "Rapid identification of mutations in a multidrug efflux pump in Pseudomonas aeruginosa."APMIS. 107. 1109-1116 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideto Tsujimoto et al.: "Diffusion of macrolide antibiotics through the outer membrane of Moraxella catarrhalis."J.Infect.Chemother.. 5. 196-200 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shah Jalal et al.: "Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients."Antimicrob.Agents Chemother.. 44. 710-712 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda et al.: "Contribution of the MexX-MexY-OprM Efflux System to Intrinsic Resistance in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 44. 710-712 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda et al: "Substrate Specificity of the MexAB-OprM, MexCD-OprJ, and MexXY-OprM Efflux Pumps in Pseudomonas aeruginosa."Antimicrob.Agents Chemother.. 44. 3322-3327 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hyunjoo Pai et al.: "Carbapenem resistance mechanisms in Pseudomonas aeruginosa clinical isolates."Antimicrob.Agents Chemother.. 45. 480-484 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhisa Masuda et al.: "Hypersusceptibility of the Pseudomonas aeruginosa nfxB mutant to □-lactams due to reduced expression of AmpC □-lactamase."Antimicrob.Agents Chemother.. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masataka Tsuda: "Use of a transposon-encoded site-specific resolution system for onstruction of large and defined deletion mutations in bacterial chromosome."Gene. 207. 33-41 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Takeuchi et al.: "Nucleotide sequence and characterization of cdrA, a cell division-related gene of Helicobacter pylori."J.Bacteriol.. 180. 5263-5268 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Mizote et al.: "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phospho-methylpyrimidine kinase."Microbiology. 145. 495-501 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] J.K.Akada et al.: "Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH."Mol.Microbiol.. 36. 1071-1084 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 後藤直正: "細菌の異物(薬剤)排出蛋白質と抗菌薬耐性"日本化学療法学雑誌. 48. 509-515 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shah Jalal et al.: "Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosaisolates from cystic fibrosis patients"Antimicrob.Agents Chemother. 44. 710-712 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhisa Masuda et al.: "Contribution of the MexX-MexY-OprM Efflux System to Intrinsic Resistance in Pseudomonas aeruginosa"Antimicrob.Agents Chemother. 44. 2242-2246 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhisa Masuda et al.: "Substrate Specificity of the MexAB-OprM, MexCD-OprJ, and MexXY-OprM Efflux Pumps in Pseudomonas aeruginosa"Antimicrob.Agents Chemother. 44. 3322-3327 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hyunjoo Pai et al.: "Carbapenem resistance mechanisms in Pseudomonas aeruginosa clinical isolates"Antimicrob.Agents Chemother.. 45. 480-484 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhisa Masuda et al.: "Hypersusceptibility of the Pseudomonas aeruginosanfxB mutant to β-lactams due to reduced expression of AmpC β-lactamase"Antimicrob.Agents Chemother.. 45(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhisa Masuda: "Interplay between Chromosomal b-lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to b-iactams is Pseudomonas aeruginosa"Antimicrob.Agents Chemother.. 43. 400-402 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kiyomi Okamoto: "Molecular cloning of oprQ encoding to the outer membrane protein OprE3 of Pseudomonas acruginosa"Microbiol.Immunol.. 43. 297-301 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naomasa Gotoh: "Topological analysis of an RND family transporter,MexD of Pseudomonas aeruginosa"FEBS Lett.. 458. 32-36 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shah Jalal: "Rapid identifcation of mutations in a multidrug efflux pump in Pseudomonas aeruginosa"APMIS. 107. 1109-1116 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideto Tsujumoto: "Diffusion of macrolide antibiotics thruough the outer membrane of Moraxella catarrhalis"J.Infect.Chemother.. 5. 196-200 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shah Jalal: "Mutations to quinolone resistance in Pseudomonas aeruginasa and Enterococcus faecjum."Drugs. 58. 119-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Naomasa Gotoh: "Multidrug efflux systems in Pseudomonas aeruginosa:their contribution to the multidrug resistance in cooperation with other resistance factors."Chin.Microbiol.Infect.. 5. 5S60-5S61 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Mizote: "Cloning and characterzation of the thiD/J gene of Escherichia cole incoding a thiamin-synthesizing bifunctional enzyme,hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."Microbiology. 145. 495-501 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masataka Tsuda: "Mobile Catabolic Genes in Bacteria."J.Biosci.Bioeng.. 87. 401-410 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shah Jalal: "Mechanisms of resistance to fluoroquinolones in Pseudomonas aeruginosa from cystic fibrosis patients."Antimicrob.AgentsChemother.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高山 吉弘: "nfxB型排出蛋白をもつPseudomonas aeruginosaに対するfosfomycinとcefepimeの併用効果"日本化学療法学雑誌. 47. 264-270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 後藤 直正: "緑膿菌の多剤耐性化に寄与する薬剤排出システム"日本化学療法学雑誌. 47. 319-328 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 後藤 直正: "キノロン耐性-複数の機構の総和"医学のあゆみ. 191. 1031-1035

    • Related Report
      1999 Annual Research Report
  • [Publications] Ramakrishnan Srikumar: "Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-Oprj in Escherichia coli." Antimicrob.Agents Chemother.42. 65-71 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 川畑敏枝: "NalB型排出蛋白質をもつ緑膿菌に対するFOMとβ-ラクタム薬の併用効果" 日本化学療法学雑誌. 46. 17-21 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroshi Yoneyama: "Subunit swapping in the Mex-extrusion pumps in Pseudomonas aeruginosa" Biochem.Biophys.Res.Commun.244. 898-902 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naomasa Gotoh: "Characterization of the MexC-MexD-OprJ multidrug efflux system in ΔmexA-mexB-oprM mutants of Pseudomonas aeruginosa." Antimicrob.Agents Chemother.42. 1938-1943 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naomasa Gotoh: "Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa." FEMS Microbiol.Lett.165. 21-27 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuhisa Masuda: "Interplay between Chromosomal b-lactamase and the MexAB-OprM Efflux System in Intrinsic Resistance to blactans in Pseudomonas aeruginosa." Antimicrob.Agents Chemother.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyomi Okamoto: "Molecular cloning of oprO encoding to the outer membrane protein OprE3 of Pseudomonas aeruginosa." Microbiol.Immunol.(in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuda,M: "Use of a transposon-encoded site-specific resolution system for construction of large and defined deletion mutations in bacterial chromosome." Gene. 207. 33-41 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi,H: "Nucleotide sequence and characterization of cdrA,a cell division-retated gene of Helicobacter pylori." J.Bacteriol.180. 5263-5268 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 津田雅孝: "トランスポゾン" 動生協会々報. 31. 54-55 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizote,T.: "Cloning and characterization of the thiDIJ gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme,hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase." Microbiology. 145. 495-501 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuda,M: "Mobile catabolic genes in basteria." J.Biosci.Bioeng.(in press).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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