| Project/Area Number |
10470120
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | The University of Tokyo (1999)
Yamaguchi University (1998) |
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Principal Investigator |
YOSHIDA Ken-ichi University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (40166947)
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| Co-Investigator(Kenkyū-buntansha) |
AKI Toshihiko Yamaguchi University, School of Medicine, Assistant professor, 医学部, 助手 (60304474)
原田 一樹 山口大学, 医学部, 助手 (00253146)
水上 洋一 山口大学, 医学部, 講師 (80274158)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | ischemia / reperfusion / stress / myocardial infarction / MAP kinase / PKC / early immediate genes / apoptosis / プロテインキナーゼC / 虚血再灌流 / MAP キナーゼ / 心筋障害 / 細胞内情報伝達 |
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| Research Abstract |
1) We found that nitric oxide protects myocardium against reperfusion injury through activation of PKC isoforms in the per fused rat heart, We also found that PKC-δ or ε isoform is involved in the protection conferred by ischemic preconditioning (that mimics the protective effect of angina against subsequent infarction) against myocardial injury by ischemia- reperfusion. We showed that hypoxia-reoxygenation activates PKC-ζ-MAP kinase- C-fos cascade that suppresses cell death due to apoptosis in myogenic cells. Two small heat shock proteins, HSP27 and MKBP translocates to the myofibrils during myocardial ischemia, while they are upregulated transiently after the birth. We also found that the genes of small HSP are under the control of a protease system proteasome and identified the transcription factor.
2) Emotional stress evoked in the rat by immobilization actuvates MAP kinase- c-fos cascade.
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